Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P61278 (somatostatin)
 22,083 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The localization and distribution of nitric oxide synthase in the hypothalamus have been studied with an immunohistochemical technique using antibodies to neuronal rat nitric oxide synthase. Subsequent double-labeling experiments examined the colocalization patterns of nitric oxide synthase and several peptides. Our results demonstrate a widespread occurrence of nitric oxide synthase-immunoreactive nerve cell bodies and processes throughout the hypothalamus, especially in various parts of the preoptic region, in the supraoptic and paraventricular nuclei, the lateral hypothalamic area, the ventromedial and dorsomedial nuclei, the arcuate nucleus and various parts of the mammillary region. Double labeling experiments showed that nitric oxide synthase-like immunoreactivity coexists with substance P-like immunoreactivity in the medial preoptic area, with oxytocin-, cholecystokinin-and galanin message-associated peptide-like immunoreactivity in the supraoptic nucleus, with enkephalin, oxytocin- and corticotropin releasing factor-like immunoreactivity in the paraventricular nucleus and with enkephalin-like immunoreactivity in the arcuate nucleus. Furthermore, in the ventromedial nucleus, nitric oxide synthase-like immunoreactivity coexisted with enkephalin-, substance P-, and somatostatin-like immunoreactivity, and in the dorsomedial nucleus with enkephalin-, galanin message-associated peptide-and substance P-like immunoreactivity. In the mammillary region nitric oxide synthase-like immunoreactivity coexisted with enkephalin-, cholecystokinin-, and substance P-like immunoreactivity. Among these neuropeptides, enkephalin and substance P were most frequently found in nitric oxide synthase-immunoreactive neurons. We conclude that nitric oxide synthase-immunoreactive neurons contain neuropeptides in various parts of the hypothalamus, and that nitric oxide in the hypothalamus may be involved in a variety of neuroendocrine and autonomic functions.
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PMID:Immunohistochemical mapping of nitric oxide synthase in the rat hypothalamus and colocalization with neuropeptides. 881 20

Double-label immunocytochemistry was used to investigate the co-localisation of neuropeptides and the enzyme nitric oxide synthase (NOS) with tyrosine hydroxylase (TH) in autonomic ganglia of the human postnatal male pelvic plexus. Postmortem specimens were obtained from six male infants and children ranging in age from 2 to 12 months who had died as a result of cot death or accidental trauma. On average, ganglia lying adjacent to the neck of the urinary bladder contained 45% of neurons which were TH-immunoreactive (-IR) while ganglia situated adjacent to the posterior and lateral aspects of the prostate gland contained 67% of neurons which were TH-IR. All the TH-IR neurons also contained dopamine beta-hydroxylase and were considered to be noradrenergic in type. On average, 61% of TH-IR neurons in bladder ganglia contained NOS, compared with 77% of non-TH-IR neurons (based on counts of over 1,000 cells in each case), while the percentages of TH- and non-TH-IR neurons containing neuropeptides were: calcitonin gene-related peptide (CGRP) (30%; 11%), neuropeptide Y (NPY) (66%; 92%), somatostatin (SOM) (70%; 29%), substance P (SP) (64%; 46%), vasoactive intestinal polypeptide (VIP) (64%; 83%). The equivalent values for TH- and non-TH-IR neurons in prostatic ganglia were NOS (38%; 59%), CGRP (55%; 18%), NPY (62%, 65%), SOM (14%, 20%), SP (13%, 8%) and VIP (42%; 82%). Varicose nerve fibers within the ganglia were seen forming pericellular arborizations around many of the ganglion cells, the most numerous containing TH-, CGRP-, NPY-, SOM- or VIP-immunoreactivity. Less common were pericellular varicosities containing SP-immunoreactivity while terminals containing NOS were not observed. No correlation could be detected between the peptide contents of the ganglion cells and of the associated pericellular terminals. However, the peptide content of the ganglion cells found in association with the urinary bladder and prostate gland correlates well with the previously documented coexistence of enzymes and neuropeptides in the intrinsic nerve fibers supplying these two regions of the human postnatal male genitourinary system.
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PMID:Co-localisation of tyrosine hydroxylase, nitric oxide synthase and neuropeptides in neurons of the human postnatal male pelvic ganglia. 881 64

The distribution of somatostatin (SOM), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), substance P (SP), tyrosine hydroxylase (a marker of noradrenergic neurons, NA) and nitric oxide synthase-immunoreactivity (NOS-IR) was examined in the inferior mesenteric ganglion of guinea pigs with double- and triple-labelling immunohistochemistry. About 75% of neurons identified were NA/SOM, almost 20% were NA/NPY and the remainder consisted of small groups of NA/- (1-5%), NA/NPY/SOM (2-5%) and VIP (1-2%) neurons. VIP neurons contained NPY-IR, usually contained SOM-IR and were surrounded by dense pericellular baskets of SP fibres. NOS-IR was found in a small proportion of neurons colocalized with VIP but both NOS- and VIP-IR were also found alone in some neurons. Some NOS reactive varicose fibres throughout the ganglia also contained VIP-IR but much of the NOS- and VIP-IR appeared to be localized in discrete varicosities. SOM-IR was also detectable in TH fibres within myenteric ganglia of the distal colon. We conclude that the subtypes of neurons in the inferior mesenteric ganglion share some properties with other sympathetic and abdominal ganglia but they exist in distinct proportions and may make dissimilar projections along the length of the gut.
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PMID:Immunohistochemically-defined subtypes of neurons in the inferior mesenteric ganglion of the guinea-pig. 883 20

The chemical coding and projections of neurons containing neuropeptide Y (NPY) have been investigated in the myenteric plexus of the guinea-pig small intestine. Chemical coding was determined by investigating the colocalization of NPY immunoreactivity with the immunoreactivities for bombesin (BN), 5-HT, nitric oxide synthase (NOS), and somatostatin. Projections were determined by studying the consequences of nerve lesions created by myectomy and myotomy operations. NPY immunoreactivity was localized in four classes of myenteric neuron, anally projecting interneurons, neurons that projected anally and to the circular muscle, neurons projecting to the longitudinal muscle and in a small population of secretomotor neurons that projected to the mucosa. The interneurons and muscle motor neurons both had Dogiel type I morphology, whereas the secretomotor neurons had fine branching processes. Of the NPY-immunoreactive Dogiel type I neurons, 98% were also immunoreactive for NOS; conversely, 82% of NOS-immunoreactive neurons were immunoreactive for NPY. BN was also colocalized with NPY and NOS; 30% of the NPY-immunoreactive neurons were BN/NOS/NPY-immunoreactive. No nerve cells had BN and NPY immunoreactivity without NOS immunoreactivity. The presence of NPY immunoreactivity was investigated in three classes of descending interneurons that are distinguished by their reactivities for somatostatin, 5-HT and NOS. NPY immunoreactivity was never colocalized with 5-HT or somatostatin, but most NPY-immunoreactive descending interneurons whose terminals formed pericellular baskets were also reactive for BN and NOS. The average projection lengths of the NPY interneurons was 2-3 mm, in the anal direction. Evaluation of immunoreactivity for BN, NOS and NPY revealed three major populations of anally directed circular muscle motor neurons, with BN/NOS/NPY, BN/NOS and NOS/NPY immunoreactivities. Examination of simultaneous labeling after nerve lesions showed that NOS/NPY neurons had short anal projections, averaging about 2-3 mm, and neurons with BN immunoreactivity were longer, having average projections of about 5-8 mm.
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PMID:Colocalization of neuropeptide Y with other neurochemical markers in the guinea-pig small intestine. 884 34

Short axon (SA) cells in the olfactory bulb are subdivided into six types after Golgi impregnation, although their functional significance is not fully elucidated. In the present study, we examined the golden hamster olfactory bulb by immunohistochemistry to localize neurotransmitters, neuron-specific marker, and nitric oxide synthase (NOS) in the SA cells. Enzyme histochemical staining was also performed to detect the activity of nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase, which is identified with NOS. In the main olfactory bulb (MOB), neuropeptide Y (NPY)-, NOS-, and NADPH-diaphorase-positive SA cells were detected in the glomerular layer (GL), vasoactive intestinal polypeptide (VIP)-positive SA cells in the external plexiform layer (EPL), and NPY-, somatostatin (SOM)-, protein gene product 9.5 (PGP 9.5)-, NOS-, and NADPH-diaphorase-positive SA cells in the granule cell layer (GCL). In the accessory olfactory bulb (AOB), VIP- and PGP 9.5-positive SA cells were detected in the mitral/tufted cell layer (MTL), and NPY-, SOM-, NOS-, and NADPH-diaphorase-positive SA cells in the GCL. The common presence of NPY- SOM-, VIP-, PGP 9.5-, NOS-, and NADPH-diaphorase-positive SA cells in both the MOB and the AOB may suggest that respective types of cells with the same immunoreactivity play the same role no matter where these cells are located in the MOB or the AOB.
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PMID:Immunohistochemical and enzyme histochemical characteristics of short axon cells in the olfactory bulb of the golden hamster. 889 91

Nitric oxide synthase is co-localized with somatostatin and neuropeptide Y in a subpopulation of striatal interneurons that stain selectively for NADPH-diaphorase. We studied the ontogeny of diaphorase-positive neurons in striatal serum-free cultures derived from 15-16-day-old CD1 mice. NADPH-diaphorase staining was detected as early as embryological day 18 in vivo and day 5 in vitro. Over the next seven days the number of neurons staining for NADPH-diaphorase increased rapidly and then levelled off at about 0.5-1% of the total neuronal population both in vivo and in vitro. The cultured diaphorase neurons were also similar to their in vivo counterparts in terms of morphology and dendritic branching. Striatal neurons expressing NADPH-diaphorase exhibit similar ontogeny, morphology and neurochemical characteristics in vivo and in serum-free primary neuronal cultures. The culture system may represent a useful model for studying this important subgroup of striatal neurons.
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PMID:The ontogeny of NADPH-diaphorase neurons in serum-free striatal cultures parallels in vivo development. 914 14

Double-label immunocytochemistry was used to investigate the colocalisation of various neuropeptides and the enzymes nitric oxide synthase (NOS) and tyrosine hydroxylase (TH) in intramural ganglia of the human male urinary bladder neck and trigone. Postmortem specimens were obtained from 7 male infants and children ranging in age from 2 mo to 3 y who had died as a result of cot death or accidental trauma. On average 60% of the intramural neurons were non-TH-immunoreactive (-IR) (i.e. presumptive cholinergic) and 40% were TH- and D beta H-IR (i.e. noradrenergic). Within the non-TH-IR population, calcitonin gene-related peptide (CGRP) was found in 65% of cells, neuropeptide Y (NPY) in 90%, nitric oxide synthase (NOS) in 45%, somatostatin (SOM) in 90%, and vasoactive intestinal polypeptide (VIP) in 40%. The corresponding values for the TH-IR neurons were CGRP (54%), NPY (70%), NOS (58%), SOM (73%) and VIP (40%). All the observed bombesin (BOM)-immunoreactivity was colocalised with TH while 90% of VIP and almost all the CGRP was colocalised with NPY. Less than 5% of neurons were immunoreactive for substance P (SP) or met-enkephalin (m-ENK) and some of these also contained TH. Varicose nerve fibres were seen in close proximity to some of the intramural neurons, the majority of such varicosities showing immunoreactivity to CGRP, VIP or TH. Less common were pericellular varicosities immunoreactive to NPY, SOM or SP. These results demonstrate the neurochemical heterogeneity of intramural neurons in the human bladder neck and provide indirect evidence for the complexity of the peripheral innervation of the human urinary bladder.
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PMID:A double-label immunohistochemical study of intramural ganglia from the human male urinary bladder neck. 903 88

A systematic search for neuroendocrine (NE) cells in the urogenital organs of the pig was carried out by means of Linder's argyrophil method and immunohistochemical techniques. The occurrence, distribution and immunohistochemical character of NE cells (paraneurons) were studied in the vaginal vestibulum, vagina, uterus, oviduct, ovary, urethra, urinary bladder and ureter. In the vestibular glands paraneurons were found to be the most numerous, while a moderate number of these cells occurred in the uterine horn and in the urethra. A distinctly smaller number of paraneurons was present in the oviduct and only occasional NE cells were observed in the urinary bladder. Immunohistochemistry was performed by using the peroxidase-antiperoxidase procedure. Different subpopulations of paraneurons were distinguishable. Chromogranin A-positive paraneurons were found in the vestibular glands, uterine horns, oviducts, urethra and urinary bladder. Somatostatin positivity was observed in NE cells of the vestibular gland, uterine horn, oviduct and urethra. The subpopulation of serotonin-positive paraneurons was present in the vestibular gland and urethra. Bombesin, vasoactive intestinal polypeptide, cholecystokinin, substance P, nitric oxide synthase, beta-endorphin, insulin, adrenocorticotropic hormone, oxytocin and thyroid-stimulating hormone antibodies gave negative reactions in the studied NE cells.
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PMID:Neuroendocrine cells in the female urogenital tract of the pig, and their immunohistochemical characterization. 909 38

In the present study we examined the distribution of chemically identified subpopulations of nonprincipal neurons in the rat hippocampus, focusing on the dorsoventral differences in their distributions. The subpopulations analyzed were those immunoreactive for parvalbumin, calretinin, nitric oxide synthase, somatostatin, calbindin D28K, vasoactive intestinal polypeptide and cholecystokinin. Using a confocal laser scanning light microscope, we could confirm that the penetration of each immunostaining, except that of calbindin D28K, was complete throughout 50 microns thick sections under our immunostaining conditions. We counted numbers of immunoreactive somata according to the 'dissector' principle, measured areas of hippocampal subdivisions and the thickness of sections, and estimated the approximate numerical densities of these subpopulations, especially for those neurons immunoreactive for nitric oxide synthase, calretinin, somatostatin and parvalbumin. Generally speaking, neurons immunoreactive for parvalbumin showed no significant dorsoventral differences in the numerical densities in any of the subdivisions of the hippocampus, whereas the numerical densities of somata immunoreactive for calretinin, nitric oxide synthase and somatostatin were significantly larger in ventral levels than at dorsal levels of the hippocampus. The numerical density of somatostatin neurons was significantly larger in ventral levels than in dorsal levels of the denate gyrus, and, although not prominent, of the CA1 region. That of nitric oxide synthase positive neurons was significantly larger in ventral levels than in dorsal levels of the CA3 region as well as of the DG but not of the CA1 region. The numerical density of calretinin positive neurons was larger in ventral levels than in dorsal levels of all hippocampal subdivisions. The present study also revealed that dorsal and ventral levels of the hippocampus differ from each other in the composition of their nonprincipal neurons.
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PMID:Distribution of nonprincipal neurons in the rat hippocampus, with special reference to their dorsoventral difference. 909 69

Certain types of cancer pain fail to respond well either to systemic drug therapy or to spinal opioids because of the occurrence of intolerable adverse effects. In addition to spinal opioids other drugs may produce an antinociceptive effect when administered by the spinal route, such as local anesthetics, NSAID, alpha 2-agonists, calcium-channel blockers, NMDA antagonists, cholinergic drugs, peptides such as somatostatin, octreotide or calcitonin, adenosine agonists, benzodiazepines, neurokinin and cholecystokinin antagonists, nitric oxide synthase inhibitors, corticosteroids, and enkephalinase inhibitors. All these drugs may be administered in combination between them, realising the so called balanced spinal analgesia. The aim of this study is to analyse: the available methods for the evaluation of pharmacological interactions, the types of interaction between different spinal antinociceptive drugs and the role of balanced spinal analgesia in the treatment of cancer pain. Analysis of the presented data shows that the spinal synergism between opioids-local anesthetics and opioids-alpha 2-agonists can be useful in the treatment of opioid refractory cancer pain. Furthermore, the use of cholinergic drugs combined with opioids and alpha 2-agonists may be promising. Finally, even if the synergism between NSAID or NMDA antagonists with opioids or alpha 2-agonists have been proved, at the moment their use in man by the spinal route is not advisable because of the absence of adequate studies on their neurotoxicity and adverse effects.
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PMID:[Balanced spinal analgesia in the treatment of oncologic pain. Review of the literature]. 910 86


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