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Query: UNIPROT:P56851 (epididymal)
 11,273 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The present study was undertaken to compare plasma lipoprotein lipid composition, as well as white adipose tissue lipoprotein lipase activity, in rats fed purified diets high in either sucrose or corn oil. The experimental diets (65% of calories as sucrose or corn oil, 15% as the opposite nutrient, and 20% as casein) were given ad libitum for 4 weeks. An additional group was fed a nonpurified diet as a reference diet. Both sucrose and oil diets were spontaneously consumed in isocaloric amounts by the animals. Despite energy intakes that were 35% lower than that of the reference group, the sucrose and oil groups exhibited final body weights that were only 6 and 9% lower, respectively, than that of the reference group, and accumulated more fat in the epididymal depots. Postprandial as well as fasting total cholesterol levels were similar in the sucrose and oil groups, while the high-density lipoprotein to total cholesterol ratio was highest in the animals fed corn oil. In both the fasted and fed states, plasma total triglyceride levels were 73% higher in the sucrose group than in the corn oil group. The largest triglyceride differences due to diet were observed in the chylomicron + very-low-density lipoprotein fraction. The oil-fed rats accumulated large amounts of triglycerides in their livers. Postprandial lipoprotein lipase activity in epididymal adipose tissue was almost twice as high in the sucrose group as in the oil group.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Plasma lipoprotein cholesterol and triglycerides and lipoprotein lipase activity in epididymal white adipose tissue of rats fed high sucrose or high corn oil diets. 353 41

Composition of gain was measured in 54 rats fed purified diets that contained either 10 or 20% casein. Rats were weighed three times weekly and their intakes adjusted so that five or six rats within each diet gained maximally or at 2, 3, 4 or 5 g/d. Live weight for each rat was 80 g at the start and 205 g when the experiment ended. Ten additional rats were killed at about 80 g to estimate initial composition. The gain contained less (P less than 0.005) water and protein and more (P less than 0.005) lipid as rate of gain increased. Gain of gastrointestinal tract was increased (P less than 0.005) with increasing rate of gain. Rats that gained faster had shorter (P less than 0.05) tibias. More (P less than 0.005) water and protein and less (P less than 0.005) lipid were in the gain of rats fed the 20% casein diet than of those fed the 10% casein diet. Rats fed the 10% casein diet had longer bodies, and those fed the 20% casein diet had longer tibias. Water and protein gain declined and tibia length was less while liver gain increased with increasing rate of gain, but all rates of change for these measurements were higher for rats fed the 10% casein diet. Dissected epididymal fat pad gain was unaffected by casein level or rate of gain. These results demonstrate that chemical composition of the total gain can be altered when rate of growth is controlled and that the epididymal fat pad is not representative of chemical composition.
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PMID:Composition of gain of rats fed low or high protein diets and grown at controlled rates from 80 to 205 grams. 369 91

Free fatty acid (FFA) mobilization during fasting was investigated in rats fed a high-protein, carbohydrate-free (HP) diet (70% casein, 8% fat, wt/wt) or a balanced diet (66% carbohydrate, 17% casein, 8% fat) for 30 to 40 days. In vivo, rats on the HP diet showed reduced rates of plasma FFA increase during fasting. Their blood sugar remained unchanged and was higher than that of control rats 24 hours after removal of food. In the fed state, serum insulin levels were smaller in HP-fed rats but did not differ significantly in the two experimental groups during fasting. In vitro, the rates of glycerol and FFA release by epididymal fat pads obtained from fasted rats were similar in rats consuming the HP diet. Fat cells isolated from rats on the HP diet also had reduced rates of basal lipolysis. Furthermore, they showed a significant increase in responsiveness to the lipolytic action of noradrenaline and an increase in both sensitivity and responsiveness to the inhibitory effect of insulin on noradrenaline-stimulated lipolysis. Adipocytes from HP-fed and control rats had mean diameters of 51 and 60 mu, respectively, and estimated average volumes of 90 and 142 pL. On the basis of existing data on the correlation between size and lipolytic activity of fat cells, the smaller size of the adipocytes from HP-fed rats might account for the lower rate of basal lipolysis but not for the increased response to the hormones. The increased sensitivity of fat cells to the antilipolytic action of insulin may have been an important factor in the reduced lipomobilization during fasting in rats under the high-protein regimen.
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PMID:Lipolysis and the antilipolytic effect of insulin in adipocytes from rats adapted to a high-protein diet. 388 Aug 58

Two protein kinases (I and II: EC 2.7.1.37) that show a high degree of substrate specificity for protamine rather than histones, phosvitin and casein were partly purified from rat epididymal tissue. The enzymes were present in the cytosol because greater than 80% of the enzymic activity was recovered in the soluble fraction. The kinases required Mg2+ for activity although Co2+ and Mn2+ were partial substitutes. Zn2+ (1 mM) inhibited nearly completely the activity of the enzymes. Both the kinases showed high affinity for activation with cyclic AMP compared to other cyclic nucleotides. Amino acid analysis of 32P-labelled protamine product revealed that the kinases transfer the terminal phosphate of ATP to serine residues of the protein. The isoenzymes I and II showed certain differences in relation to their hydroxyapatite-chromatography profiles, pH activation profiles, heat sensitivity and Km for ATP and cyclic AMP.
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PMID:Purification and properties of cyclic AMP-dependent protein kinase from rat epididymis. 628 28

Synthesis of fatty acids was measured in the liver and in epididymal adipose tissue of sand rats and albino rats. In chow-fed sand rats the rate of hepatic lipogenesis, as measured by the incorporation of 3H2O into fatty acids, was four- to sevenfold higher than in albino rats and in sand rats on a low-calorie saltbush diet. The contribution of [14C]glucose to lipogenesis in sand rat liver was lower than in albino rats. In fed sand rats lipogenesis incorporating 3H2O was stimulated by casein but not by glucose. In adipose tissue, lipogenesis measured 1 h after administration of 3H2O was much lower in sand rats than in albino rats. In vitro incorporation of [14C]glucose or acetate into adipose tissue fatty acids was negligible. In adipose tissue, uptake of very-low-density lipoproteins (VLDL) and lipoprotein lipase activity were sevenfold higher than in albino rats. Activities of NADP-malate dehydrogenase, acetyl CoA carboxylase, and fatty acid synthetase were considerably higher in the liver of chow-fed sand rats than in albino rats. It was concluded that obesity in sand rats originates from hepatic lipogenesis without a significant contribution of local fatty acid synthesis in adipose tissue.
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PMID:Lipogenesis in the sand rat (Psammomys obesus). 634 15

Rates of fatty acid synthesis were assessed in carcass, liver, and adipose tissue from rats fed for 30 to 40 days a balanced diet (66% wt/wt carbohydrate, 17% casein, 8% fat) or a high-protein carbohydrate-free diet (70% casein, 8% fat). Despite similar body weight increases, carcass fatty acid content of rats on the high-protein (HP) diet was 13% less, and the weight of their epididymal fat pads was reduced by 29% in relation to the controls. In vivo incorporation of 3H2O into carcass fatty acids (FA) and into liver triacylglycerol (TAG) was significantly reduced in HP-fed rats. FA synthesis from 14C-acetate, glucose, or leucine and from 3H2O was also markedly decreased in liver slices from HP rats. The amount of 3H-TAG that accumulated in plasma of rats injected with triton WR 1339 to block peripheral utilization of lipoprotein corresponded in HP and control rats to only 4.1% and 5.0%, respectively, of 3H-FA recovered in carcasses from animals not treated with triton, indicating that almost all of the carcass 3H-TAG was synthesized in situ. However, on a long term basis, the reduced hepatic lipogenesis and the resulting decreased transport of TAG might affect lipid accumulation in HP rats. In vivo lipogenesis from 3H2O and in vitro FA synthesis from 3H2O and from 14C-precursors did not differ significantly in retroperitoneal and epididymal adipose tissue from HP and control rats. In both groups of animals, in vivo rates of lipogenesis were higher in retroperitoneal than in epididymal adipose tissue but still did not account for rates of FA synthesis by carcasses, suggesting the existence of other sites with higher lipogenic activity.
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PMID:Reduced lipogenesis in rats fed a high-protein carbohydrate-free diet. 669 61

To test the effects of trans unsaturated fatty acids (t-FA) on atherosclerosis, lipidemia and enzyme activities, rabbits were fed a semipurified, cholesterol-free diet containing 40% sucrose, 25% casein and 14% fat for 5 months. Two experimental diets provided either 6% (high) or 3.2% (low) t-FA. The control group was fed a fat of composition similar to the two experimental diets but free of t-FA. Serum cholesterol and triglycerides were elevated in the rabbits fed 6% t-FA. Liver glycerides were also elevated in this group. The fatty acids of plasma, erythrocytes, epididymal fat, liver microsomes and liver mitochondria reflected the dietary composition. Levels of aortic atherosclerosis were identical in the three groups. There were no significant differences in activity of five hepatic enzymes: glucose-6-phosphatase (microsomal), fatty acid synthetase (cytosolic), malate dehydrogenase, beta-hydroxybutyrate dehydrogenase and monoamine oxidase (mitochondrial).
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PMID:Influence of trans unsaturated fats on experimental atherosclerosis in rabbits. 683 52

Skeletal muscle growth, muscle nucleic acids, and muscle protein synthesis and breakdown were measured to evaluate the protein requirement of weanling rats. Male Sprague-Dawley rats were fed purified diets that differed in protein and energy contents. Diets ranged from 5 to 30% casein, 5 to 15% corn oil, and all were methionine fortified. All diets were provided for 14 days beginning at 24 days of age. At 38 days of age, animals were killed and the soleus, plantaris, gastrocnemius, extensor digitorum longus (EDL), and biceps brachii muscles and the inguinal, epididymal, and perirenal fat pads were removed and weighed. The soleus and EDL were used to determine protein synthesis and breakdown and nucleic acid contents. Body weight, muscle weight, protein synthesis, DNA and RNA/DNA increased as the casein content of the diet increased to 15%. Casein levels up to 30% failed to cause any additional increases in any of these parameters. These findings indicate that for growing rats maximum protein synthesis defines the minimum about of dietary protein necessary to achieve maximum growth.
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PMID:Responses of skeletal muscle protein synthesis and breakdown to levels of dietary protein and fat during growth in weaning rats. 705 63

The effect of experimental protein malnutrition and of subsequent refeeding at various ages on growth, adipose tissue development, body composition and serum triiodothyronine (T3) concentration were studied in weanling, male Sprague-Dawley rats fed isoenergetic diets containing 22% casein (controls) or 8% casein (PM-3) from 3 until 33 weeks of age. In addition, groups of control rats were switched to the 8% casein diet at 7 weeks of age (PM-7), and groups of PM-3 rats were refed the control diet from 7 (RF-7) or 11 (RF-11) weeks of age. At 33 weeks of age, PM-3 rats were 56% and PM-7 rats 82% the weight of controls, while RF-7 rats were 84% and RF-11 77% the weight of controls, respectively. Carcass fat and energy content corresponded to body weight in all groups. The weights of the epididymal fat pads of control rats did not increase after 24 weeks of age. The epididymal fat pad weights of all other groups remained smaller than those of controls, but continued to increase throughout the study. Epididymal adipocyte numbers did not change after 11 weeks of age in control rats or after 17 weeks of age in PM-7 and RF-7 rats, but continued to increase throughout the 33 weeks in the PM-3 and RF-11 rats. Adipocyte size increased with age in all groups but remained smaller throughout in PM-3 rats. Serum T3 concentrations were greatest in the PM-3 rats, intermediate in the PM-7 rats and became normalized in both RF groups by 33 weeks of age. These studies confirm that the postweaning protein deprivation impairs the growth of lean tissues and indicate that its primary effect on adipose tissue is a decrease in adipocyte proliferation and/or differentiation which may not recover with refeeding.
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PMID:Effect of prolonged experimental protein malnutrition and of refeeding on growth, adipose tissue development and body composition in rats. 725 98

Feeding of a glycerine rich diet with sufficient supply of essential amino acids (mixture of casein and gelatine) causes a significant reduction of the serum triglyceride level in rats. To study some aspects of this triglyceride lowering effect the in vivo incorporation of C14-1-acetate and C14-1-palmitate into the serum and liver lipids as well as the lipoprotein lipase and the hormone sensitive lipolytic activity of the epididymal adipose tissue in vitro were estimated. After feeding the gelatine containing diet the incorporation of labelled acetate and palmitate into the serum triglycerides was significantly decreased with no change of the incorporation into the liver triglycerides and without detectable accumulation of liver lipids. The decreased serum triglyceride level of the serum corresponded to a decreased lipoprotein lipase activity in the epididymal adipose tissue, whereas the basal and norepinephrine stimulated lipolysis of this tissue was not affected. From these results it is concluded that due to feeding of a glycine rich diet the triglyceride secretion of the liver is decreased leading to a lower serum triglyceride level.
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PMID:The hypolipaemic action of a glycine rich diet in rats. 742 87


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