Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P56851 (epididymal)
 11,273 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

It was demonstrated that Ledakrin, an antitumour drug, causes mobilization of free fatty acids and glycerol from the epididymal adipose tissue of rat in vitro. The disproportion observed in the release of glycerol and free fatty acids following Ledakrin administration suggested a biphasic effect of this drug, with initial stimulation and later inhibition of the processes of lipolysis and lipogenesis. Blockade of adrenergic membrane receptors (with propranolol or regitine) abolished the lipolytic effect of Ledakrin.
Acta Physiol Pol
PMID:Ledakrin effect on free fatty acid and glycerol mobilization from epididymal adipose tissue of rat in vitro. 53 74

The studies of the morphology and development of the testicular artery in cattle have been carried out on 95 preparations--85 male fetuses from the 8-40th week of pregnancy and 10 neonates at the age of 1 to 14 days of life. The age of the fetuses has been determined by Kantorova method supplemented with the methods of other authors. The observations have been made using rubber latex introduced into the examined arteries by means of the automatic device of our own design. On the basis of the performed examinations the following generalizations, conclusions and nomenclature suggestions can be made: Testicular arteries in cattle, in most cases, develop from the last pair of mesonephric lumbar arteries (aa. mesonephridicae lumbales) in embryo and through a number of evolutionary changes in the course of development transform from the simple form characteristic of the youngest fetuses (8-10 weeks) into the form approximate in the new born animals to the final form typical of the adult cattle. The examined arteries were formed in the process of quantitative and qualitative transformations which occurred, both, in the main trunk of the testicular artery, and its permanent and temporary secondary branches. Transformations of this kind are typical of developmental changes which either become more prominent or disappear with age. The formation of the characteristic of sex and species shape of the testicular artery results from these transformations and is determined by the physiological and hemodynamic needs of the testicle and epididymis, which are supplied by that artery only. Taking into account the shape and topography of the testicular artery it is possible to distinguish the following three sections in its finally formed main trunk: 1) straight part (pars recta), 2) convoluted part (pars convoluta) and 3) marginal part (pars marginalis). Temporary branches such as uretral branch (ramus uretericus) and renal capsule branch (ramus capsularis renis) are derivatives of the straight part of the studied artery. From the convoluted part of the testicular artery there arise as permanent vessels--branch of the ductus deferens (ramus ductus deferentis) and epididymal branch (ramus epididymalis) and, additionally, as a temporary vessel--I order accessory testicular branch (ramus testicularis I ordinis accessorius). The marginal part of the testicular artery divides into two final branches of the examined vessel. i.e., medial and lateral testicular branches of I order. Further order derivatives of them enter parenchyma of the testicle and form within that organ its own blood circulation. (ABSTRACT TRUNCATED AT 400 WORDS)
Pol Arch Weter 1990
PMID:[Morphology and development of the bovine testicular artery during fetal and neonatal periods]. 213 48

The effect of oral hypoglycaemic drugs, SPC-703 [n-(p-toluenesulphonyl)-5-methyl-2-pirazoline-1-carbonami de] and tolbutamide on insulin binding by rat adipocytes from epididymal fat pads were studied. SPC-703 and tolbutamide in concentration of 1 mM added in vitro to the suspension of adipocytes had no effect on insulin binding and kinetic parameters of insulin receptors. Daily administration of 300 mg/kg body weight of SPC-703 or tolbutamide for 10 days resulted in 48% and 34% increase of specific binding of insulin by adipocytes, respectively. From the Scatchard plot it appears that the increase of binding resulted from increased affinity of insulin receptors. These results may explain extrapancreatic action of sulphonylurea derivatives.
Acta Biochim Pol 1984
PMID:Effect of sulphonylurea derivatives, SPC-703 and tolbutamide, on insulin binding by isolated rat adipocytes. 638 86

The aqueous extract of rat salivary submaxillary gland was found to contain three protein fractions activating the release of free fatty acids (FFA) and glycerol from rat epididymal adipose tissue in vitro. Physico-chemical investigations of these proteins demonstrated certain common features: all three fractions were albumins having a common isoelectric point, and their aqueous solutions absorbed light at the same wavelength. The use of lipolysis activators and inhibitors (theophylline, propranolol, insulin) for investigating their effects on FFA and glycerol release produced by these protein fractions explained the mechanism of the lipolytic action of the protein fractions from rat submaxillary glands.
Acta Physiol Pol
PMID:Certain characteristic features of the lipolytic factor from rat submaxillary salivary glands. 638 45

In rats receiving intraperitoneally Ledacrine in toxic doses the free fatty acids (FFA) and glycerol serum levels and the amount of fatty acids and glycerol released from the epididymal tissue of the rats were determined during one-hour incubation in vitro. It was found that Ledacrine in toxic doses stimulated the process of lipolysis as evidenced by raised serum FFA and glycerol levels and increased amount of FFA and glycerol released from the epididymal adipose tissue in vitro in relation to the values in control rats. The results of investigations of acid-base equilibrium parameters indicated that Ledacrine in toxic doses caused in rats metabolic acidosis associated with respiratory acidosis.
Acta Physiol Pol
PMID:Ledacrine effect on free fatty acids and glycerol mobilization from rat adipose tissue in vivo. 730 98

Till the end of March 1998 first seven trials of MESA-ICSI (microsurgical epididymal sperm aspiration and intracytoplasmic sperm injection) in the Ist Chair and Clinics of Obstetrics and Gynecology of the Silesian Medical Academy in Bytom were performed in cooperation with an urologist from the IInd Chair and Clinics of Urology in Zabrze in couples suffering from sterility caused by obstructive azoospermia. In all the cases both contraindications and conditions of MESA-ICSI were respected. Fertilization rate of the MII oocytes was on average 62% and cleavage rate of the fertilized oocytes 76.9%. After embryo transfer at least two pregnancies occurred, from them one is ongoing well. The result of the last procedure is not known yet. The data obtained permit to conclude, that MESA-ICSI makes a real chance to possess their own children by couples with sterility caused by obstructive azoospermia.
Ginekol Pol 1998 Jun
PMID:[The usefulness of MESA-ICSI (microsurgical epididymal sperm aspiration and intracytoplasmic sperm injection) in cases of obstructive azoospermia]. 969 53

Medicine is going from success to success, but with the ever-increasing expectations of the society, the more it achieves, the less it satisfies. This is also true for reproductive medicine. Indeed, advances in basic research and the practical applications of the research that have been performed since the first successful in vitro fertilisation (IVF) in 1978, have given rise to hitherto unimaginable strategies for achieving human reproduction. Couples, that were considered sterile only a few years ago, can now acquire assistance conceiving. Twenty two years after Louise Brown was born, the reproductive medicine methods have spread all over the world and thousands of children have been born as a result of these techniques. ICSI applied to epididymal or testicular sperm in cases of azoospermia permits infertile men to become fathers. Even if the results of reproductive medicine have improved in terms of numbers of pregnancies, it is still striking that it is necessary to use stimulation which sometimes leads to hyperstimulation and multiple pregnancies, that embryo development in vitro is still limited, that implantation only occurs for 15-20% of embryos and this ratio have not changed in the last 20 years. The pregnancy rates currently obtained with IVF are at the most similar to those occurring in vivo. We still need to improve techniques to gain pregnancy rates approaching 50% per embryo. In order to fullfil this task, we need to introduce less aggressive and much simpler ovarian stimulation, routine culture of embryos to the blastocyst stage, pre-implantation genetic diagnosis, cryopreservation and in vitro maturation of oocytes and finally to improve the implantation rates by our ability to understand and control the dialog between blastocyst and receptive endometrium.
Ginekol Pol 2001 Jan
PMID:[Assisted reproductive medicine in the next age]. 1138 3

Maedi Visna Virus (MVV) is the etiological agent of a systemic disease of sheep, which causes lesions in lungs, the central nervous system, joints, and mammary glands. It has been speculated that the association with Brucella ovis may lead to the venereal shedding of the virus. In this work, samples of epididymis from ten rams positive for MVV and infected experimentally with Brucella ovis, were subjected to liquid-phase PCR, immunohistochemistry (IHC) and in situ PCR tests, aimed at identifying the pathogens in a tissue context. IHC was carried out using a monoclonal antibody raised against p28 MVV protein and a polyclonal antibody to B. ovis. Liquid phase- and in situ PCR were designed to amplify a portion of MVV proviral DNA Pol sequence. In the animals showing B. ovis-related histopathological changes, IHC clearly demonstrated a positivity for B. ovis and MVV in interstitial and epithelial ductal cells. In situ PCR assessed the presence of MVV proviral DNA in macrophages and elements inside the epithelium. The unaffected and reagent control samples constantly gave negative results. Taken together, these data demonstrate that MVV may affect ovine epididymis, apparently taking advantage of the concurrent infection by B. ovis. The tropism of MVV for the epididymal epithelial cells, may be responsible for its excretion with the semen.
 ...
PMID:Association of Maedi Visna virus with Brucella ovis infection in rams. 1277 12

Lipid disorders are one of the known metabolic changes associated with chronic renal failure (CRF) [1, 2]. They are present as: hypertriglyceridemia--existed in 60% of CRF patients and hypercholesterolemia observed in 20-30% of people with this syndrome. These disorders, what was shown also in our own studies, are existing in different intensity in patients treated with maintenance haemodialysis [3], peritoneal dialysis [4] and after renal transplantation as well [5]. Mechanism of hypertriglyceridemia, despite over thirty years of studies, is still not finally elucidated. The opinion that it is a result of impaired triglyceride removal (due to decreased activities of both lipoprotein and hepatic lipases) is well documented, however the role of lipogenesis in its development is obscure [6, 7]. The reports concerning this problem contain contradictory data. In our studies performed several years ago we have shown that lipogenesis rate in white adipose tissue of uremic rats is significantly augmented [8, 9, 10] due to activation of free fatty acid synthase. Therefore, recently we paid once again our attention on the activity of this lipogenesis rate limiting enzyme responsible for the long term regulation. We measured its activity, protein abundance and mRNA level in liver and epididymal white adipose tissue of rats with surgically induced renal failure (two-stage subtotal nephrectomy). The results support the thesis that lipogenesis takes a part in a hypertriglyceridemia found in renal failure. There have been observed a significant increase in plasma triglyceride and VLDL concentrations in uremic animals and it was associated with the increase of FAS activity, FAS protein abundance and FAS mRNA. The results were similar in both studied tissues. Moreover, there have been also observed the increased activities of malic enzyme, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. All these enzymes participate in NADPH production, which is a necessary substrate for fatty acid biosynthesis [11, 12, 13]. Concluding, it appears that the rise in plasma triglyceride and VLDL concentrations observed in CRF rats is not only the result of increased liver and white adipose tissue lipogenesis rate. One has to remember, that these date are strictly original and enabling to elucidation further pathogenesis of hyperlipidemia in CRF. In the second set of experiments performed also in rats with experimentally induced CRF we have found that hypercholesterolemia observed in those animals is dependent on the significant activation of cholesterol synthase, induced by increased production of this enzyme (increment of protein abundance and synthase mRNA [14, 15]. Simultaneously, we have performed original studies on the diurnal rhythm of cholesterologenesis, showing that activity of this process is significantly augmented during whole twenty four hours [15]. Summarizing, one have to underline that our observations have important impact to the elucidation of lipid disturbances pathomechanism. Nevertheless further studies are necessary to establish how experimental data are corresponding with human pathology.
Pol Merkur Lekarski 2003 Oct
PMID:[Pathomechanism of hyperlipoproteinemia in chronic renal failure]. 1497 58

The present investigation was an attempt to evaluate the effect of aflatoxin on biochemical and histopathological changes in the epididymis of mice and its possible amelioration on pre-treatment with vitamin E. Adult male albino mice were orally administered with 25 and 50 mg of aflatoxin/animal/day (750 and 1500 mg/kg body weight) for 45 days. Epididymis was isolated and processed for biochemical analysis. As compared with the control, absolute and relative epididymal weights were significantly reduced in aflatoxin-treated mice. Aflatoxin treatment caused significant, dose-dependent reduction in protein and sialic acid contents in caput and cauda epididymis than that of vehicle control. While activities of succinic dehydrogenase and adenosine triphosphatase were significantly reduced, acid phosphatase activity was significantly higher in caput and cauda epididymis of aflatoxin-treated mice than that of vehicle control. Pyknosis of epithelial cell nuclei, disorganization of epithelium, clumping of stereocilia and lumen devoid of sperms in caput and cauda epididymis were observed. Thus, pre-treatment with vitamin E (2 mg/0.2 mL olive oil/ animal/day) significantly ameliorated aflatoxin-induced changes, measured by biochemical and histopathological parameters.
Acta Pol Pharm
PMID:Vitamin E ameliorates aflatoxin-induced alterations in the epididymis of mice. 1864 52


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