Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P56851 (epididymal)
 11,273 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

After castration, there was a marked decrease in serum androgen concentration at 6 h, and a dramatic inhibition of ornithine decarboxylase (ODC) at 12 h. Administration of testosterone propionate to castrated rats at a dose of 0.05 mg/animal restored ODC activity to the normal value. However, no change was observed when intact rats were treated with testosterone even at a 40-fold higher dose, indicating that endogenous androgens present in intact rats are far in excess for maintenance of maximal levels of activity. Administration of the antiandrogen flutamide to intact rats caused a moderate decrease in epididymal weight, whereas this effect was more pronounced in castrated, androgen-treated rats. In the latter, the effect of flutamide was significant at the lowest dose used (0.5 mg/day). ODC activity was significantly decreased by flutamide treatment of intact rats, but even at the highest dose used (10 mg/day) only a 39% inhibition was observed. In flutamide-treated rats, LH concentrations were markedly increased, as were serum and epididymal androgens. In androgen-treated castrated rats, flutamide caused epididymal ODC to fall to undetectable values. These results show that: (1) androgens are essential for the maintenance of ODC activity in the epididymis; (2) epididymal ODC activity is maximally stimulated by endogenous androgens, at least in the pubertal rat; (3) the apparent potency of flutamide is substantially lowered by an increase in epididymal androgens. We suggest that ODC is a sensitive marker of the action of androgens and antiandrogens in the epididymis.
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PMID:Ornithine decarboxylase activity as a marker of androgen and antiandrogen action in the rat epididymis. 339 35

Ornithine decarboxylase (ODC) activity was measured in epididymides of 45-day-old rats. Higher ODC activity was detected in the corpus and cauda than in the caput epididymidis. Bilateral castration for 7 days caused epididymal ODC to fall to undetectable values, whereas testosterone restored activity to normal values. The effect of the androgen was significantly inhibited by cyproterone acetate. The caput was more sensitive to the action of testosterone than were the corpus and caudal segments. Unilateral castration for 4 or 8 days did not affect ODC on the control or castrated side, but the activity fell in epididymides of both sides after removal of the remaining testis. These results show that epididymal ODC activity is androgen-dependent.
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PMID:Androgen-dependence of ornithine decarboxylase in the rat epididymis. 382 Jan 86

We have employed a monospecific, polyclonal antibody to ornithine decarboxylase (ODC) for the immunocytochemical localization of ODC in freshly isolated testicular cells, epididymal spermatozoa, and cultured Sertoli cells. Antigenically detectable material was present in the cytoplasm of all cell types tested and was highly concentrated in the acrosomal vesicle of round spermatids and in the acrosome region of epididymal spermatozoa. The specific enzymatic activity of ODC, as measured biochemically, was much higher in the interstitial cells than in the other testicular cell types, and no ODC activity was detected in the epididymal spermatozoa or in the Sertoli cells after 5 days in culture. These studies showed that, while all testicular cell types studied contained ODC-like immunoreactive molecules, only testicular germ cells and interstitial cells exhibited detectable ODC activity.
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PMID:Localization of ornithine decarboxylase in rat testicular cells and epididymal spermatozoa. 390 26

Regional differences in the total activities of the epididymal secretory parameter, alpha-glucosidase, were demonstrated in the 20,000 g supernatants of human epididymal homogenates. A comparison of the enzymic activities in the supernatants and the washings from 12 one centimetre segments of human epididymides indicated an activity peak in segments 3-4 which appeared to be largely intracellular and which presumably reflects the acidic isoenzyme. A second peak in the caudal region, the segmental localization of which was more variable and differed in post-mortem and operation specimens, appeared to be primarily intraluminal. The activities of ornithine decarboxylase (ODC), the rate-limiting enzyme in the polyamine pathway, also exhibited regional differences with higher activities in relatively short segments in both caput and caudal portions. Corresponding alterations were also found in the tissue concentrations of the enzyme products, spermidine and spermine. The increased intracellular activities of ODC and alpha-glucosidase in the distal caput segments presumably reflect the transition of epithelium from the efferent to the epididymal ducts.
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PMID:Segmental distribution of alpha-glucosidase, ornithine decarboxylase and polyamines in the human epididymis. 850 29

The antiandrogens flutamide and casodex have been administered subcutaneously (vehicle, 1, 5 or 10 mg per day) to prepubertal male rats for 10 days. A significant change of epididymal weight has been observed after both treatments, from the lowest dose used. Epididymal dihydrotestosterone concentrations were significantly increased in flutamide- or casodex-treated rats, while epididymal 3 alpha-androstanediol concentrations were affected only after flutamide administration, suggesting a differential effect on androgen metabolism between both antiandrogens. Ornithine decarboxylase (ODC) activity was significantly decreased by flutamide, and to a lesser extent by casodex. Antiandrogen administration resulted in a significant decrease in epididymal content of the polyamines putrescine, spermidine, and spermine. A slight but significant decrease in putrescine and spermidine concentrations, but not in spermine, was observed after flutamide treatment. However, casodex had no effects on polyamines levels. A decrease in putrescine concentration was detected only when ODC activity fell to rather low levels. Interference of the antiandrogens with the biological action of androgens on ODC activity was clearly seen in immature male rats. Therefore, both epididymal growth and differentiation, in correlation to ODC activity, would be severely affected at an early period of sexual development, such as prepuberty.
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PMID:Ornithine decarboxylase activity and polyamine levels in the epididymis of prepubertal rat after antiandrogen administration. 904 38