Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P56851 (
epididymal
)
11,273
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Growth hormone's (GH) lipolytic activity in white adipose tissue (WAT) results in decreased body fat in giant GH transgenic mice and increased subcutaneous fat in dwarf growth hormone receptor/binding protein gene-disrupted mice (GHR -/-). We therefore hypothesized that GH action would affect expression of
CIDE-A
(cell-death-inducing DFF45-like effector-A), a protein found in white adipose tissue (WAT) and involved in lipid metabolism.
CIDE-A
RNA levels were determined in subcutaneous, retroperitoneal and
epididymal
adipose tissue isolated from wild-type and GHR -/- mice. The adipose tissue was also analyzed for adipocyte size. We determined that the lack of GH action has depot-specific effects on the levels of
CIDE-A
RNA and affected adipocyte cell size.
CIDE-A
expression is significantly reduced in GHR -/- subcutaneous fat compared to wild-type but is not altered in retroperitoneal or
epididymal
fat. Likewise, adipocytes are significantly enlarged in GHR -/- subcutaneous adipose tissue relative wild-type mice. A high-fat diet also influenced the level of
CIDE-A
RNA in mouse adipose tissue. The high-fat diet significantly reduced
CIDE-A
expression in wild-type subcutaneous fat but did not alter
CIDE-A
expression in subcutaneous fat of GHR -/- mice. The diet also reduced
CIDE-A
expression in wild-type retroperitoneal fat but the levels of
CIDE-A
in
epididymal
fat were unchanged. In contrast, the high-fat diet reduced
CIDE-A
expression in both retroperitoneal and
epididymal
fat of GHR -/- mice. These data demonstrate that
CIDE-A
levels are reduced in two different mouse models of obesity and this reduction may contribute to altered lipid metabolism.
...
PMID:CIDE-A gene expression is decreased in white adipose tissue of growth hormone receptor/binding protein gene disrupted mice and with high-fat feeding of normal mice. 1754 97
