Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P56851 (epididymal)
 11,273 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The alterations of the epididymal F-zone caused by apical mesotestis bilateral cutting after scrotal exposition were studied in adult albino rats. The epididymis were removed at 2, 4, 7, 14, 21 and 28 days after surgery. With a semi-automatic program, from a IBAS 2000 image analyzer, areas, perimeters and maximum and minimum diameters were measured from both the tubular and the lumen sections of the caput epididymal F-zone. The animals with cutting of the apical mesotestis showed a significative increase with respect to controls for all the considered variables, indicating a tubular dilatation as a consequence of the surgical intervention. The mast cells present in the connective tissue of the F-zone of the control animals presented a typical fluorescent colour with Acridine Orange, while this was not observed in the animals with cutting of the apical mesotestis.
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PMID:[Morphometric studies of the epididymal duct in the F-zone after apical mesotestis cutting]. 324 89

Inasmuch as caput epididymal and even testicular spermatozoa are now being used to generate pregnancies by direct injection into the oocyte, differences in the chromatin of spermatozoa from proximal and distal locations in the epididymis were studied. Acridine Orange staining was used to investigate chromatin structure in human spermatozoa which had left the testis and were undergoing maturation in the epididymis. Measurement of green and red fluorescence intensities of human spermatozoa by flow cytometry demonstrated a decrease in binding of Acridine Orange to DNA as the spermatozoa traversed the epididymis. Using spermatozoa from the cauda epididymis as the standard, the percentages of spermatozoa from the efferent duct, proximal corpus epididymis, midcorpus epididymis, distal corpus epididymis, proximal cauda epididymis and distal cauda epididymis that had matured with regard to chromatin condensation were 28 +/- 5, 39 +/- 3, 49 +/- 5, 64 +/- 5, 69 +/- 6 and 74 +/- 4% respectively. It may be concluded that eggs fertilized by ejaculated spermatozoa receive a more highly condensed form of chromatin than that received by eggs inseminated with proximal epididymal or testicular spermatozoa.
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PMID:Changes in chromatin condensation of human spermatozoa during epididymal transit as determined by flow cytometry. 867 86

The quality of sperm chromatin is an important factor in fertilization and is especially critical where one spermatozoon is artificially selected for fertilizing an egg (as in intracytoplasmic sperm injection). In this study, flow cytometry after staining of human spermatozoa with Acridine Orange was used to study chromatin structure. A method is described for estimating the percentage of cells in a human sperm sample that have completed epididymal maturation in regard to chromatin condensation. Of the 121 samples of the semen that were examined, nine contained a higher percentage of hypocondensed spermatozoa and six samples contained elevated amounts of hypercondensed spermatozoa. In addition to aberrancies in chromatin condensation other defects showed up as satellite populations of spermatozoa with higher than normal ratios of red/green fluorescence after Acridine Orange staining. Such defects were found in 15 semen samples. The use of swim-up and Percoll gradient centrifugation methods was shown to improve the percentage of spermatozoa with normal chromatin structure in some samples with poor initial quality.
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PMID:Evaluation of chromatin condensation in human spermatozoa: a flow cytometric assay using acridine orange staining. 923 7