Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P56851 (epididymal)
 11,273 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

To examine the precise localization of lysosomal cysteine proteinases, cathepsins B, H, and L in rat epididymal epithelial cells, immunohistochemistry and enzyme assay were applied to the epididymal tissue. Granular immunodeposits for cathepsins B and H were detected in epididymal epithelial cells, whereas faint or no immunoreactivity for cathepsin L was found. Moreover, immunoreactivity for cathepsin B appeared mainly in principal cells and was more intense in the head of the epididymis than in the tail, whereas that for cathepsin H appeared in both principal and clear cells and was more intense in the tail than the head. By enzyme assay, activities of cathepsins B and H showed a similar distribution to that of the immunoreactivity. The cathepsin L-specific activity was distributed evenly in each part of the epididymis and was also detected in epididymal fluids obtained from the body and tail parts. By immunoblotting, proforms of cathepsins B, H, and L were present in the seminal fluid. The results suggest that cathepsins B and H are involved in the intracellular degradation system of epididymal epithelial cells, and proforms of cathepsins B, H, and L may be secreted into the epididymal duct lumen.
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PMID:Lysosomal cysteine proteinases in rat epididymis. 830 58

The expression of cathepsin H (CH) in differentiating rat spermatids was studied by an immunoelectron microscopic technique. Cathepsin H was detected in the acrosome throughout differentiation steps but cathepsins B, D, and L and lysosomal membrane protein (LGP107) were not. Early in the formation of the acrosome, CH signals were observed in Golgi vesicles but not in acrosomal vesicles. At steps 3-4, CH signals were associated with a fibrous material attached to the inner surface of the vesicle membrane on the Golgi side. At steps 5-6, this fibrous material accumulated to form an electron-dense sheet to which CH signals were confined. The rest of the acrosome was negative for the enzyme. At steps 11-12, the CH-positive fibrous sheet expanded from the apical to the ventral side of the sperm head. After step 16, the surface of outer dense fibers in the flagellar axoneme and reticulated bodies were stained for CH. In epididymal sperm, CH signals were detected in the acrosome as well as on the surface of the outer dense fibers running from the middle to the principal piece. By immunofluorescence staining, CH was found to be localized to the acrosome, middle piece, and principal piece.
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PMID:Expression of cathepsin H in differentiating rat spermatids: immunoelectron microscopic study. 1283 26