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Query: UNIPROT:P56851 (epididymal)
 11,273 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A study of the lipid composition of ram testicular and ejaculated spermatozoa was made in an attempt to resolve conflicting results in the literature. Testicular spermatozoa were found to contain more than double the amount of phospholipid present in ejaculated spermatozoa. Most phohpholipid components, including choline plasmalogen, decrease substantially in concentration during migration of the spermatozoa through the male reproductive tract. Phosphatidylserine, ethanolamine phosphoglycerides and cardiolipin components accounted for the greatest relative decreases in concentration, the former decreasing by approximately nine tenths. Of the phospholipid-bound fatty acids the most pronounced change occurs in palmitic during migration of spermatozoa through the reproductive tract. There is a net loss of approx. 500 mug of palmitic acid for every 10-9 spermatozoa. The loss of arachidonic acid was particularly interesting, and prompted a study of the prostaglandin content of testicular and epididymal fluids, since arachidonic acid can act as a precursor of prostaglandin. The concentration of prostaglandin F2alpha found in the testicular and epididymal fluid is considerably in excess of that found in venous plasma of the ram.
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PMID:Changes in phospholipids of ram spermatozoa during migration through the epididymis and possible origin of prostaglandin F2alpha in testicular and epididymal fluid. 112 97

The question of whether diplasmalogens [1,2-di(O-1'-alkenyl) phosphatidyl derivatives] make up part of the plasmalogen component of cell phospholipids was examined using rabbit epididymal spermatozoa. These cells are readily obtained as a highly homogeneous suspension and long have been known to have high plasmalogen content. Phospholipids were determined by thin layer chromatography (TLC) with CuSO4 staining. Plasmalogens were determined by hydrolysis of the phospholipids with TCA/HCl, followed by TLC and CuSO4 staining. Ethanolamine derivatives were determined by ninhydrin. The phosphatidylethanolamine (PE) content of these cells was 29 +/- 2 micrograms/10(8) cells, 90% of which was assayed as diplasmalogen and 10% as diacyl PE. No monoplasmalogen could be detected. The presence of diplasmalogen as the major component of PE was given further support from infrared and proton nuclear magnetic resonance (1H-NMR) spectroscopy, which showed the presence of O-1'-alkenyl substituents but near absence of O-acyl substituents. The phosphatidylcholine (PC) content of the cells was 104 +/- 5 mu/10(8) cells, of which 50% was monoplasmalogen with the 1'-alkenyl group on the 2 position of the glycerol moiety. No diplasmalogen was found in PC. The other phospholipids in rabbit sperm were phosphatidylglycerol (PG), cardiolipin (CL), sphingomyelin (SP) and lysophosphatidylcholine (LPC). Phosphatidylserine (PS) and phosphatidylinositol (PI) were present at the limits of detectability of the TLC method. None of these phospholipids contained plasmalogen. The PE component of rabbit sperm phospholipids appears to differ from that of the other cells in having the previously unreported diplasmalogen as its major constituent.
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PMID:Evidence for diplasmalogen as the major component of rabbit sperm phosphatidylethanolamine. 409 18