Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P56851 (epididymal)
 11,273 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Dietary fish oils, enriched with omega-3 fatty acids (e.g., MaxEPA fish oil), inhibit lipogenesis and have a marked hypotriglyceridemic effect in man and experimental animals. Dietary omega-3 fatty acids also reduce adipose tissue trophic growth in rats. To understand the metabolic basis for this, we measured the effect of fish oil feeding upon rat plasma triglyceride concentration, fat pad mass, fat cell size, fat cell lipolysis, as well as lipoprotein binding to adipocyte plasma membranes. In adolescent (250 g) male Wistar rats fed 20% (w/w) fish oil supplemented diets for 3 weeks, plasma triglyceride levels and epididymal and perirenal fat pad mass were significantly (P less than 0.005) reduced compared to pair-fed controls given 20% lard diets. These differences in fat pad mass between the diets were greater than differences in whole animal mass or in the mass of livers, testes, kidneys, spleens, or hearts. Isoproterenol-stimulated lipolysis was significantly (P less than 0.005) higher in fish oil fed rats than in pair-fed controls. In young (100 g) rats plasma triglyceride levels were 10 times lower in the fish oil fed group after 5 weeks as compared to the lard-fed controls. This was accompanied by a reduction in epididymal and perirenal fat pad mass as well as a 2-3-fold decrease in adipocyte volumes; there was no significant difference between the two groups in fat cell number in each region. Plasma membranes of epididymal adipocytes from fish oil fed rats bound significantly (P less than 0.001) less HDL1 than the lard-fed rats, possibly as a result of a reduction in fat cell size and/or alteration of plasma membrane structure. Thus in both young and old rats, the reduction in plasma triglyceride concentration in conjunction with increased hormone-stimulated lipolysis may explain in part the selective reduction in adipose tissue trophic growth accompanying fish oil consumption.
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PMID:Dietary fish oils modify adipocyte structure and function. 165 18

Compared with diets high in saturated and monounsaturated fatty acids (20% lard by weight), diets high in polyunsaturated fatty acids (20% sunflower oil) alter the fatty acid composition of rat adipocyte plasma membranes and enhance HDL2 binding. We examined the effect of these two diets on HDL1 and HDL2 apolipoprotein and cholesterol uptake by adipocytes isolated from perirenal and epididymal adipose tissue of male Wistar rats. Consistent with selective cellular uptake. HDL esterified cholesterol uptake was 3-10-fold higher than predicted from HDL apolipoproteins associated with adipocytes. Dietary polyunsaturated fatty acids significantly enhanced apolipoprotein and esterified-cholesterol uptakes from HDL2 by perirenal adipocytes. This effect of dietary fat composition was adipose-region (perirenal greater than epididymal) and HDL-subfraction (HDL2 greater than HDL1) specific. Thus, diet-induced changes known to alter membrane phospholipid composition and increase HDL2 binding are also associated with enhanced HDL2-esterified-cholesterol uptake by adipocytes.
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PMID:Dietary polyunsaturated fatty acids enhance the uptake of high-density lipoprotein cholesterol ester by rat adipocytes. 237 95

The interaction of high-density lipoproteins (HDL) with adipocytes is important in the regulation of cellular cholesterol flux. To study the mechanisms of HDL binding and cellular processing, we incubated adipocytes isolated from epididymal and perirenal adipose tissue of male Wistar rats (300 g) with HDL1 (1.07-1.10 g/mL) and HDL2 (1.10-1.14 g/mL) fractions separated from rat plasma by gradient ultracentrifugation. Freshly isolated adipocytes were incubated with 125I-labeled HDL for 2 h at 37 degrees C to determine cell-associated uptake and degradation. Adipocytes from both fat regions showed significant cell-associated HDL1 and HDL2 uptake and very high medium degradation (2- to 6-fold higher than uptake). To assess 125I-labeled HDL binding independent of cellular metabolism, we purified adipocyte plasma membranes from isolated adipocytes and used them in binding assays. Binding of HDL1 and HDL2 in the membrane system was 85-95% specific, sensitive to high NaCl concentrations, and abolished by pronase treatment. In contrast to HDL2 binding, the maximum HDL1 binding to perirenal plasma membranes was significantly higher than its binding to epididymal membranes (7.2 +/- 1.3 vs. 4.4 +/- 0.2 micrograms/mg, n = 6, p less than 0.05). This increment in HDL1 binding to perirenal membranes represented an EDTA- sensitive, calcium-dependent component. These results indicate that HDL binding to adipocyte plasma membranes depends on both adipose tissue region and HDL subtype. The membrane binding characteristics, taken together with the cellular uptake results, suggest that adipocytes bind and metabolize HDL and that this interaction may involve a protein receptor.
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PMID:Characterization of high-density lipoprotein binding to rat adipocytes and adipocyte plasma membranes. 314 99

Effects of early over- and undernutrition on lipoprotein profiles of adult Swiss male mice reared in litters of different sizes were investigated. Lipoproteins were isolated by density gradient ultracentrifugation and defined by chemical composition. Protein moieties were defined by their changes. The lipoprotein lipase (LPL) activity in epididymal adipose tissue, heart and diaphragm was measured. Early feeding patterns induced permanent body weight differences in adult mice. Serum phospholipid content was significantly higher in obese than in control mice. Overfeeding led to significantly higher activity of LPL in adipose tissue; inversely, undernutrition induced a lower LPL activity. There was a trend toward variations of lipoprotein concentrations in relation to litter size, with significant differences being observed only between obese and undernourished mice for LDL-HDL1 (low density lipoprotein--high density lipoprotein) and HDL2 concentrations. Compared with normally fed mice the most notable alterations in plasma lipoprotein composition were, in LDL-HDL1, greater cholesteryl ester in obese and less phospholipid in undernourished mice. In contrast, tetramethylurea-soluble apolipoprotein distribution was unaffected by litter size. Although moderate differences were observed in lipoprotein compositions and levels in over- or undernourished mice, further investigations of lipoprotein metabolism and metabolic abnormalities in this animal model are required.
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PMID:Serum lipoprotein profiles in mice: effects of early over- and undernutrition. 318 66