Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P51812 (mitogen-activated protein)
 10,636 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

To investigate the molecular mechanisms of signaling transduction by which Pseudomonas pyocyanin induces IL-8 expression in human airway epithelial cells, A549 and SPC-A-1 cells were challenged with P. aeruginosa conditioned medium or pyocyanin. Chemokine interleukin-8 (IL-8) release from the challenged cells was measured by ELISA, and Western blot was performed to analyze the degradation of IkappaB-alpha and the phosphorylation of MAPKs (mitogen-activated protein kinases) in the extracts from cells stimulated with pyocyanin. Both of P. aeruginosa conditioned medium and pyocyanin remarkably increased IL-8 expression by human airway epithelial cells. Degradation of IkappaB-alpha was found shortly after A549 cells were stimulated with pyocyanin. Western hybridization analysis also demonstrated that pyocyanin caused phosphorylation of MAPKs including ERK1/2, p38 and JNK in A549 cells. Pretreatment of A549 cells with U0126 (10 micromol/L), a selective inhibitor of MEK1/2 (ERK1/2 kinase) or with SB203580 (10 micromol/L), a specific inhibitor of p38 MAPK, diminished the pyocyanin-induced IL-8 production. These findings suggest that Pseudomonas pyocyanin can increase IL-8 expression by human airway epithelial cells through MAPKs signaling pathways and the activation of NF-kappaB is also involved in this process.
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PMID:[Involvement of MAPKs and NF-kappaB pathways in Pseudomonas pyocyanin-induced interleukin-8 expression by human airway epithelial cells]. 1584 70

Tea polyphenols (-)-epigallocatechin-3-gallate (EGCG) and theaflavin-3-3'-digallate (TF3) are two prospective compounds in cancer prevention and treatment. Ascorbic acid (Vc) is essential to a healthy diet as well as being a highly effective antioxidant. In this work, the effects of the combination of EGCG or TF3 with Vc on the apoptosis and caspases-3/9 activities in human lung adenocarcinoma SPC-A-1 cells and esophageal carcinoma Eca-109 cells were determined. Furthermore, the role of mitogen-activated protein kinases (MAPK) pathways in the apoptosis induced by TF3 or EGCG together with Vc were studied using three MAPK inhibitors (ERK inhibitor PD98059, JNK inhibitor SP600125 and p38 inhibitor SB203580). Our results showed that Vc could enhance the EGCG and TF3 induced apoptosis in SPC-A-1 and Eca-109 cells, and this effect involved the activation of caspase-3 and 9. EGCG, TF3 and Vc could activate MAPK pathways respectively, and each compound activated different MAPK subfamilies in different cells. This may explain the enhancement of EGCG and TF3 induced apoptosis by Vc in SPC-A-1 and Eca-109 cells, and will ultimately aid the design of more effective anti-cancer treatments.
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PMID:Enhancement of (-)-epigallocatechin-3-gallate and theaflavin-3-3'-digallate induced apoptosis by ascorbic acid in human lung adenocarcinoma SPC-A-1 cells and esophageal carcinoma Eca-109 cells via MAPK pathways. 2389 41