Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P51532 (transcriptional activator)
 6,546 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Plasmid pP51 of Pseudomonas sp. strain P51 contains two gene clusters encoding the degradation of chlorinated benzenes, tcbAB and tcbCDEF. A regulatory gene, tcbR, was located upstream and divergently transcribed from the chlorocatechol oxidative gene cluster tcbCDEF. The tcbR gene was characterized by DNA sequencing and expression studies with Escherichia coli and pET8c and appeared to encode a 32-kDa protein. The activity of the tcbR gene product was analyzed in Pseudomonas putida KT2442, in which it appeared to function as a positive regulator of tcbC expression. Protein extracts of both E. coli overproducing TcbR and Pseudomonas sp. strain P51 showed specific DNA binding to the 150-bp region that is located between the tcbR and tcbC genes. Primer extension mapping demonstrated that the transcription start sites of tcbR and tcbC are located in this region and that the divergent promoter sequences of both genes overlap. Amino acid sequence comparisons indicated that TcbR is a member of the LysR family of transcriptional activator proteins and shares a high degree of homology with other activator proteins involved in regulating the metabolism of aromatic compounds.
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PMID:Characterization of the Pseudomonas sp. strain P51 gene tcbR, a LysR-type transcriptional activator of the tcbCDEF chlorocatechol oxidative operon, and analysis of the regulatory region. 205 Jun 30

The TcbR transcriptional activator protein, which is encoded by the tcbR gene of Pseudomonas sp. strain P51 (J. R. van der Meer, A. C. J. Frijters, J. H. J. Leveau, R. I. L. Eggen, A. J. B. Zehnder, and W. M. de Vos, J. Bacteriol. 173:3700-3708, 1991), was purified from overproducing Escherichia coli cells by using a two-step chromatographic procedure. Subsequent use of TcbR in gel mobility shift assays with progressively shortened portions of a DNA fragment containing the divergent promoter sequences of the tcbR gene and the tcbCDEF operon showed that the direct binding site of TcbR is located between positions -85 to -40 relative to the tcbCDEF transcriptional start site, containing a LysR-type recognition sequence motif (T-N11-A). DNase I footprinting experiments revealed that TcbR protected an area on both strands of the intercistronic region which was actually larger than this binding site (from positions -74 to -24). This stretch of protected DNA was interrupted by a region (positions -52 to -37) which became strongly hypersensitive to DNase I digestion upon addition of TcbR, suggesting that TcbR induces a bend in the DNA at this site.
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PMID:Analysis of the binding site of the LysR-type transcriptional activator TcbR on the tcbR and tcbC divergent promoter sequences. 814 50