Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P51532 (transcriptional activator)
 6,546 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We constructed an in vivo reporter system to measure the activity of CooA as the transcriptional activator and showed that the recombinant CooA was active as the transcriptional activator in the presence of CO even in E. coli cells. A dominant positive mutant of CooA, in which Met131 was replaced by Leu, was isolated by a random mutagenesis with this reporter system. The electronic absorption spectra of M131L mutant were identical to those of wild type CooA in oxidized (Fe3+), reduced (Fe2+), and CO-bound (CO-Fe2+) state, indicating that the coordination structure and environment of the heme were not changed by this mutation. Methionine at position 131 was the carboxyl-terminal end of the heme-binding domain of CooA, which would be adjacent to the hinge region connecting the heme-binding domain and the DNA-binding domain.
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PMID:Single transduction in the transcriptional activator CooA containing a heme-based CO sensor: isolation of a dominant positive mutant which is active as the transcriptional activator even in the absence of CO. 939 45

Redox modification of functional or regulatory proteins has emerged as an important mechanism of post-translational modification. However, methionine sulfoxide reductase (Msr)-mediated redox modification of transcription factor in regulating physiological processes in plants, especially fruit ripening, remains unclear. Here, we identified that MaNAC42, a transcriptional activator, is involved in the regulation of fruit ripening in banana under oxidative stress. Integrated analysis of ChIP-qPCR and EMSA data showed that MaNAC42 directly binds to promoters of oxidative stress- and ripening-related genes. Ectopic over-expression of MaNAC42 in Arabidopsis delays the dark-induced senescence in leaves, indicating that MaNAC42 plays a negative role in senescence. Furthermore, we found that MaNAC42 is a target of MaMsrB2, a methionine sulfoxide reductase B. Methionine oxidation in MaNAC42, i.e. sulfoxidation, or mimicking sulfoxidation by mutating methionine to glutamine, leads to decreased DNA-binding capacity and transcriptional activity. On the other hand, MaMsrB2 can partially repair oxidized-MaNAC42 and restore its DNA-binding capacity. In conclusion, our study proposes a novel regulatory mechanism of fruit ripening in banana involving in MaMsrB2-mediated redox regulation of ripening-related transcription factor MaNAC42.
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PMID:Sulfoxidation regulation of MaNAC42 transcription factor influences its functions in relation to stress-induced fruit ripening in banana. 3307 Jan 85