Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P51532 (transcriptional activator)
 6,546 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Phenazine methosulfate (PMS), a generator of superoxide, evoked the transcription of cas2 and cefF, ultimately resulting in the enhanced biosyntheses of clavulanic acid (CA) and cephamycin C (CMC) in Streptomyces clavuligerus. The transcriptional activation of cas2 and cefF was accompanied with that of ccaR, a regulatory gene for biosyntheses of CA and CMC. PMS or H2O2 in cell-free extract exerted a positive regulation on in vitro protein phosphorylation. The PMS-mediated activation of protein phosphorylation was significantly offset by butylated hydroxyanisole, a radical scavenger. Staurosporine, a protein kinase inhibitor, was shown to have a negative effect on PMS-promoted CA accumulation. Therefore, it is suggestive that PMS-activated transcription of cas2 and cefF is mediated by protein phosphorylation and the expression of a pathway-specific transcriptional activator as found in other streptomycetes. These experimental results present an example of the functional relationship between oxidative stimuli and secondary metabolite production in streptomycetes.
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PMID:Molecular basis for enhanced biosynthesis of clavulanic acid by a redox-cycling agent, phenazine methosulfate, in Streptomyces clavuligerus. 1064 26