Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P51532 (transcriptional activator)
 6,546 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Using a modified TAIL-PCR technique, the 5'-flanking regions of the phenylalanine ammonia lyase (Pal) genes of a yam species, Dioscorea bulbifera, and the phosphoglucose isomerase (Pgi) gene of D. tokoro were successfully isolated. Two novel modifications of the TAIL-PCR procedure introduced here, namely (1) the use of a battery of random 10-mers (RAPD primers) as short arbitrary primers, and (2) the use of a total of five nested, gene-specific primers, allow the rapid isolation of the 5'-flanking region of any gene from organisms with large genomes. Isolated 5'-flanking regions were fused to the gus gene, and tested for transient expression in tobacco BY2 cells. All the isolated 5'-flanking regions were shown to drive reporter gene expression. Three Pal promoters responded to salicylic acid, presumably as a result of the binding of a MYB transcriptional activator to the multiple MREs (Myb Recognition Elements) present in these regions.
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PMID:Rapid isolation of promoter sequences by TAIL-PCR: the 5'-flanking regions of Pal and Pgi genes from yams (Dioscorea). 1082 Nov 91

Global transcription studies have identified a large number of redox-responsive genes, although the biological relevance of this regulation has not been experimentally tested. In particular, several genes coding for enzymes involved in glucose metabolism have been identified as redox-responsive in Escherichia coli. However, only zwf, which codes for glucose-6-phosphate dehydrogenase, has been shown experimentally to affect the cellular resistance to oxidative stress. We addressed the question of whether ptsG, coding for the membrane component of the glucose-specific transporter system, and pgi, coding for phosphoglucose isomerase, two additional genes identified in whole-genome functional screens, are indeed relevant in antioxidant defense. PTS assays showed that glucose transport was induced under oxidative stress elicited by the superoxide-producing agent paraquat (PQ). This induction of glucose transport under oxidative stress was dependent on the soxRS genes, coding for a sensor- transcriptional activator system, and ptsG. The binding of purified SoxS to the ptsG promoter region was shown by gel mobility-shift assay, and the activation of the ptsG promoter P1 was demonstrated by primer extension assays. Finally, a ptsG mutant strain was hypersensitive to PQ when grown in rich medium plus glucose, but not in rich medium without glucose. The pgi gene showed the same pattern of regulation by oxidative stress under the control of the SoxRS system, and a strain carrying a pgi deletion was hypersensitive to PQ.
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PMID:Activation of glucose transport under oxidative stress in Escherichia coli. 1836 88