Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P50583 (asymmetrical)
 12,197 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Recent studies in Jurkat T cells and in rat basophilic leukemia cells revealed an Mg(2+)-inhibited cation (MIC) channel that has electrophysiological properties similar to TRPM7 Eyring rate model expressed exogenously in mammalian cells. Here we compare the characteristics of several polyvalent cations and Mg(2+) to block monovalent MIC current from the outside. Putrescine, spermidine, spermine, PhTX-343 (a derivative of the naturally occurring polyamine toxin philanthotoxin), and Mg(2+) each blocked in a dose- and voltage-dependent manner, indicating a blocking site within the electric field of the ion channel. Spermine and the relatively bulky PhTX-343 exhibited voltage dependence steeper than that expected for the number of charges on the molecule. Polyamines and Mg(2+) are permeant blockers, as judged by relief of block at strongly negative membrane potentials. Intracellular dialysis with spermine (300 microM) had no effect, indicating an asymmetrical pore. At the single-channel level, spermine and Mg(2+) induced flickery block of 40-pS single channels. I/V characteristics and polyamine block are similar in expressed TRPM7 and in native MIC currents, consistent with the conclusion that native MIC channels are composed of TRPM7 subunits. An Eyring rate model is developed to account for I/V characteristics and block of MIC channels by polyvalent cations from the outside.
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PMID:Polyvalent cations as permeant probes of MIC and TRPM7 pores. 1266 38

In order to evaluate the impact of semi-intensive shrimp farming, comparisons between Control and Impact areas were made based on multiple lines of evidence using an asymmetrical design. Water and sediment samples were collected in four shrimp farms located in Todos os Santos Bay, Bahia, Brazil. Nutrients, trace elements and macrobenthic assemblages were evaluated using uni- and multivariate analyzes. Significant differences were observed between Impact and Control areas for the water column dataset (i.e., ancillary variables, SPM, dissolved nutrients and major and trace elements in SPM), whereas no significant differences were observed for the chemistry of sediments. Macrobenthic assemblages were negatively affected by shrimp farm activities. Impacted sites presented the lowest abundance, richness and different structure of macrofaunal benthic assemblages. Farms clearly produced negative impacts in the Todos os Santos Bay. This conclusion was only possible to be reached through the use of multiple lines of evidence. Chemistry and benthic assemblages data combined produced a better description of the quality and impacts of the evaluated environments. Different conclusions would have been reached if chemistry and ecology results were studied separately vs. together.
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PMID:Detection of environmental impacts of shrimp farming through multiple lines of evidence. 2737 96