Gene/Protein Disease Symptom Drug Enzyme Compound
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 12,197 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We describe the synthesis and characterization of degradable nanogels that display bulk erosion under physiologic conditions (pH = 7.4, 37 degrees C). Erodible poly(N-isopropylmethacrylamide) nanogels were synthesized by copolymerization with N,O-(dimethacryloyl) hydroxylamine, a cross-linker previously used in the preparation of nontoxic and biodegradable bulk hydrogels. To monitor particle degradation, we employed multiangle light scattering and differential refractometry detection following asymmetrical flow field-flow fractionation. This approach allowed the detection of changes in nanogel molar mass and topology as a function of both temperature and pH. Particle erosion was evident from both an increase in nanogel swelling and a decrease in scattering intensity as a function of time. Following these analyses, the samples were recovered for subsequent characterization by direct particle tracking, which yields hydrodynamic size measurements and enables number density determination. Additionally, we confirmed the conservation of nanogel stimuli-responsivity through turbidity measurements. Thus, we have demonstrated the synthesis of degradable nanogels that erode under conditions and on time scales that are relevant for many drug delivery applications. The combined separation and light scattering detection method is demonstrated to be a versatile means to monitor erosion and should also find applicability in the characterization of other degradable particle constructs.
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PMID:Monitoring the erosion of hydrolytically-degradable nanogels via multiangle light scattering coupled to asymmetrical flow field-flow fractionation. 2000 Jun 62

Biological importance of antiseptic drug; O-Benzyl hydroxylamine was explored using QSAR studies for ultimate usage for treating fungal infections. In this research work, the molecular spectroscopic tool and theoretical calculation method of analysis. The data acquired from both tools were evaluated and compared to validate structural and vibrational characteristics. Mulliken charge displacement around molecular site in order for exploring electronic properties to find out the cause of inducement of drug potential. The Lipinski rule of five was evaluated for the measurement of biological importance of the drug compound. The lipophilicity and topological surface area of the drug was monitored for determining biological process activity. The partial involvement of compositional bonds of the molecule was appraised for influential vibrational characteristics. The chemical environment for making chemical property was monitored from the uniform and asymmetrical chemical shift of core and allied carbons. The resultant oscillating potential orientation in the molecular site was identified and the residing zones were recognized to find out the origin of drug potential. The occurrence of CT complex process was studied and the CTC was found to be CC and C-N for generating drug activeness. The enhancement of hyper active polarization was measured in first and second order from which the charge level pulling on different entities were observed for ensuring the biological affinity of the compound. The enantiomer characteristics were thoroughly studied to measure the level of toxicity.
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PMID:Analysis on biological importance of antiseptic drug, O-Benzyl hydroxylamine, by the application of spectroscopic and theoretical tools. 3168 52