Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P50583 (asymmetrical)
 12,197 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A double immunocytochemical method combining the preembedding avidin biotin peroxidase complex technique and the postembedding immunogold technique was used to examine synaptic interactions between GABAergic and nitric oxide synthase containing neurons in the same tissue sections of the dorsal raphe nucleus of the Wistar white rat. Although a large number of immunogold stained GABAergic axon terminals were found to be presynaptic to dendrites containing nitric oxide synthase-like immunoreaction product, synapses between GABA-like immunoreactive axon terminals and nitric oxide synthase-like immunoreactive perikarya were rare. The labeled boutons were found to make symmetrical and asymmetrical synapses. No axo-axonic synapse was found. These results suggest that GABAergic neurons could modulate nitric oxide producing neurons in the dorsal raphe nucleus through direct synaptic relations.
 ...
PMID:Electron microscopic study of GABAergic synaptic innervation of nitric oxide synthase immunoreactive neurons in the dorsal raphe nucleus in the rat. 898 44

We characterized presubicular neurons giving rise to bilateral projections to the medial entorhinal cortex (MEA) of the rat. Retrograde labeling of presubiculo-entorhinal projections with horseradish peroxidase and subsequent GABA immunocytochemistry revealed that 20-30% of the ipsilaterally projecting neurons are GABAergic. No GABAergic projections to the contralateral MEA were observed. GABAergic projection neurons were observed only in the dorsal part of the presubiculum, which, when taking into account the topography of presubicular projections to MEA, indicates that only the dorsal part of MEA receives GABAergic input. The GABAergic projection neurons constitute approximately 30-40% of all GABAergic neurons present in the superficial layers of the dorsal presubiculum. Using double-label fluorescent retrograde tracing, we found that the ipsilateral and contralateral presubiculo-entorhinal projections originate from different populations of neurons. Anterograde labeling of presubiculo-entorhinal projections and electron microscopical analysis of labeled terminals substantiated the presence of a restricted GABAergic presubiculo-entorhinal projection. A small fraction of afferents to only ipsilateral dorsal MEA formed symmetrical synapses with dendritic shafts. No symmetrical synapses on spines were noted. Most afferents to the dorsal part of ipsilateral MEA, as well as all afferents to the remaining ipsilateral and contralateral MEA, formed asymmetrical synapses with both spines and dendritic shafts in an almost equal ratio. Thus, we conclude that the majority of the presubiculo-entorhinal projections exert an excitatory effect on both principal neurons and interneurons. The projections from the dorsal part of the presubiculum comprise a small inhibitory component that originates from GABAergic neurons and targets entorhinal interneurons.
 ...
PMID:GABAergic presubicular projections to the medial entorhinal cortex of the rat. 898 7

A light and electron microscopic study, combining HRP axonal tracing or degeneration and GABA immunocytochemistry, was performed in the lamprey Lampetra fluviatilis in order to analyze retinal and non-retinal inputs upon the retinopetal neurons localized in the reticular mesencephalic area (RMA). The iontophoretic deposit of HRP onto the central stump of the cut optic nerve produced a dense anterograde labeling in the retino-recipient strata marginale and cellular externum of the optic tectum as well as the retrograde labeling of retinopetal neurons in the mesencephalic tegmentum. The large ascending proximal dendrites of the retinopetal neurons constituted a distinct bundle coursing first dorso-laterally in the dorsal mesencephalic tegmentum, and then dorso-medially in the strata fibrosum centrale and cellulare et fibrosum internum of the optic tectum before their distal portions penetrated the retino-recipient tectal layers. The distribution of GABA immunoreactivity was also investigated in the tectal layers and dorsal mesencephalic tegmentum with both pre- and post-embedding methods. The retinal terminals, identified either following HRP iontophoresis in the optic nerve or in early phases of degeneration after short-term survivals following retinal lesion, contained rounded-shaped synaptic vesicles and were always GABA immunonegative. They established asymmetrical synaptic contacts on the distal dendrites of RMA neurons and represented 11.4% of all terminals contacting such neurons (15% of these neurons were GABA immunopositive). The dense extra-retinal input upon the retinopetal RMA neurons was composed of five types of axon terminal profiles, either GABA-immunopositive or -immunonegative. Considering the different cytochemical types of axon terminals contacting RMA neurons, as well as the characteristics of the retinal targets of these neurons, we suggest that, globally, the effects of RMA neurons upon the retina are mainly inhibitory.
 ...
PMID:Retinal and non-retinal inputs upon retinopetal RMA neurons in the lamprey: a light and electron microscopic study combining HRP axonal tracing and GABA immunocytochemistry. 900 48

To characterize glutamate/aspartate uptake activity in various cellular and subcellular elements in the striatum, rat striatal slices were exposed to 10 and 50 mu M exogenous (D)-aspartate. After fixation with glutaraldehyde/formaldehyde the distribution of (D)-aspartate was analysed by postembedding immunocytochemistry and the ultrastructural distribution was compared with the distributions of endogenous glutamate and GABA. Light microscopically, (D)-aspartate-like immunoreactivity was localized in conspicuous dots along very weakly labelled dendritic profiles and neuron cell bodies. At the electron microscope level gold particles signalling (D)-aspartate occurred at highest density in nerve terminals making asymmetrical contacts with postsynaptic spines (i.e. resembling synapses of cortical afferents). Astrocytic processes also contained gold particles, but at a lower density than nerve endings. In contrast, dendritic spines were only weakly (D)-aspartate-positive. The difference in labelling at 10 and 50 mu M (D)-aspartate was consistent with 'high-affinity' uptake. Neighbouring sections processed with other antibodies showed that the D-aspartate labelling. Occurred in nerve terminals strongly immunoreactive for glutamate, rather than in terminals very weakly glutamate-immunopositive or in nerve endings immunoreactive for GABA. Glutamate labelling of perfusion-fixed striatum confirmed that terminals forming asymmetrical synaptic contacts with spines were enriched with gold particles, suggesting that these terminals use glutamate as a transmitter. This study demonstrates that high-affinity uptake sites for excitatory amino acids in the striatum are most strongly expressed on presumed glutamatergic nerve terminals and on astrocytes.
 ...
PMID:Selective excitatory amino acid uptake in glutamatergic nerve terminals and in glia in the rat striatum: quantitative electron microscopic immunocytochemistry of exogenous (D)-aspartate and endogenous glutamate and GABA. 908 27

The lobus parolfactorius (LPO) has been implicated in memory formation associated with passive avoidance training of young posthatch domestic chicks. The anatomical circuitry underlying memory formation in the chick is likely to involve the intermediate medial hyperstriatum ventrale-archistriatum-LPO arc. In the present work, we attempted to combine an ultrastructural characterisation of archistriatal afferent terminals in LPO with a description of the synaptic structure of LPO, in particular those elements that are immunoreactive to glutamate and GABA. Ventral archistriatal regions of 7-day-old domestic chicks were iontophoretically injected with Phaseolus vulgaris leucoagglutinin and the anterograde transport of the tracer was detected in the LPO. Selected samples from these birds, and also from other day-old chicks, were resin-embedded and reacted for L-glutamate or GABA, using the postembedding immunocytochemical method. Glutamate was abundant in the neuropil of LPO and typically seen in axodendritic or axospinous terminals with asymmetrical junctions, often multiple or perforated postsynaptic appositions. Conversely, GABA was often present in aspinous dendrites, probably representing GABAergic local circuit neurons or (putative striatonigral) projection neurons. Archistriatal efferents terminating in LPO formed small en passant or terminal varicosities, with infrequent asymmetrical axospinous synapses. Glutamate was not detected in these boutons. The findings imply that the functional state of LPO, based on powerful glutamatergic excitation, may be modified by a non-glutamatergic archistriatal input.
 ...
PMID:Synaptic terminals immunolabelled against glutamate in the lobus parolfactorius of domestic chicks (Gallus domesticus) in relation to afferents from the archistriatum. 909 42

The distribution of glycine immunoreactivity in the lamprey (Lampetra fluviatilis and Ichthyomyzon unicuspis) spinal cord was studied at the light and electron microscopic levels by use of postembedding techniques and antibodies against glutaraldehyde-conjugated glycine. To determine if glycine may be co-stored with other amino acid transmitters, the levels of glycine immunolabeling in identified GABAergic and glutamatergic synapses were examined. The most intense glycine labeling occurred in axon profiles of different diameter distributed throughout the ventral and lateral columns, with the highest density in the areas bordering the lateral cell column. Intermediate levels of glycine labeling were present in certain interneurons in the lateral cell column and in stretch receptors (edge cells) at the lateral margin of the spinal cord. Most other cell bodies, including glutamatergic dorsal cells, were virtually unlabeled. Examination of adjacent sections incubated with GABA antiserum revealed that many of the glycine-containing cells and fibers also contained high levels of GABA. At the ultrastructural level, the glycine immunolabeling was accumulated in two morphologically distinct types of terminal, one of which co-contained GABA. The terminals which exhibited glycine, but not GABA immunoreactivity, contained flattened synaptic vesicles and formed symmetrical synaptic specializations. The terminals that exhibited both GABA and glycine labeling contained pleomorphic synaptic vesicles and had either symmetrical or asymmetrical synaptic specializations. In both cases the glycine labeling was accumulated over the synaptic vesicles. Examination of identified glutamatergic axons in glycine-labeled sections did not provide any evidence for an accumulation of glycine in the synaptic vesicles or other structures of these exons. The present study provide the first morphological description of the localization of glycine in the lamprey spinal cord. The results confirm previous physiological and pharmacological studies, which have implicated glycine as a major fast inhibitory transmitter in the interneuronal network for locomotion, and in a proportion of stretch receptor neurons. The data also show that a significant proportion of the GABAergic synapses, but not the glutamatergic synapses, may utilize glycine as co-transmitter.
 ...
PMID:Immunocytochemical localization of glycine in the lamprey spinal cord with reference to GABAergic and glutamatergic synapses: a light and electron microscopic study. 912 8

Previous immunocytochemical studies in the cerebral cortex of various species have shown that the calcium-binding protein calretinin (CR) labels specific subpopulations of nonspiny nonpyramidal cells (interneurons). The present study attempts to characterize morphologically and chemically the microcircuitry of CR-immunoreactive (CR-ir) neurons in the human temporal neocortex. Postembedding immunocytochemistry for CR and GABA and combination immunocytochemistry for CR and nonphosphorylated neurofilament protein (NPNFP) or for CR and the calcium-binding proteins parvalbumin (PV) and calbindin (CB) showed CR multiterminal endings frequently innervating the distal apical dendrite or the cell body and proximal dendrites of NPNFP-ir or CB-ir pyramidal cells, respectively. Cell bodies of interneurons immunoreactive for CB or PV were innervated only occasionally by CR multiterminal endings, whereas certain GABA neurons were surrounded by them. Furthermore, CR-ir axon terminals formed either symmetrical (the majority) or asymmetrical synapses with a variety of postsynaptic elements. These results indicate that different subpopulations of CR interneurons exist that are specialized for selective innervation of somatic or dendritic regions of certain pyramidal and nonpyramidal neurons.
 ...
PMID:Synaptic connections of calretinin-immunoreactive neurons in the human neocortex. 918 52

The arborization pattern and postsynaptic targets of corticofugal axons in basal forebrain areas have been studied by the combination of anatomical tract-tracing and pre- and postembedding immunocytochemistry. The anterograde neuronal tracer Phaseolus vulgaris leucoagglutinin was iontophoretically delivered into different neocortical (frontal, parietal, occipital), allocortical (piriform) and mesocortical (insular, prefrontal) areas in rats. To identify the transmitter phenotype in pre- or postsynaptic elements, the tracer staining was combined with immunolabeling for either glutamate or GABA, or with immunolabeling for choline acetyltransferase or parvalbumin. Tracer injections into medial and ventral prefrontal areas gave rise to dense terminal arborizations in extended basal forebrain areas, particularly in the horizontal limb of the diagonal band and the region ventral to it. Terminals were also found to a lesser extent in the ventral part of the substantia innominata and in ventral pallidal areas adjoining ventral striatal territories. Similarly, labeled fibers from the piriform and insular cortices were found to reach lateral and ventral parts of the substantia innominata, where terminal varicosities were evident. In contrast, descending fibers from neocortical areas were smooth, devoid of terminal varicosities, and restricted to the myelinated fascicles of the internal capsule en route to more caudal targets. Ultrastructural studies obtained indicated that corticofugal axon terminals in the basal forebrain areas form synaptic contact primarily with dendritic spines or small dendritic branches (89%); the remaining axon terminals established synapses with dendritic shafts. All tracer labeled axon terminals were immunonegative for GABA, and in the cases investigated, were found to contain glutamate immunoreactivity. In material stained for the anterograde tracer and choline acetyltransferase, a total of 63 Phaseolus vulgaris leucoagglutinin varicosities closely associated with cholinergic profiles were selected for electron microscopic analysis. From this material, 37 varicosities were identified as establishing asymmetric synaptic contacts with neurons that were immunonegative for choline acetyltransferase, including spines and small dendrites (87%) or dendritic shafts (13%). Unequivocal evidence for synaptic interactions between tracer labeled terminals and cholinergic profiles could not be obtained in the remaining cases. From material stained for the anterograde tracer and parvalbumin, 40% of the labeled terminals investigated were found to establish synapses with parvalbumin-positive elements; these contacts were on dendritic shafts and were of the asymmetrical type. The present data suggest that corticofugal axons innervate forebrain neurons that are primarily inhibitory and non-cholinergic; local forebrain axonal arborizations of these cells may represent a mechanism by which prefrontal cortical areas control basal forebrain cholinergic neurons outside the traditional boundaries of pallidal areas.
 ...
PMID:Cortical input to the basal forebrain. 921 67

Synaptic contacts between noradrenaline (NA) neurons and GABA (gamma-aminobutyric acid) afferents and/or substance P (SP) afferents in the locus coeruleus (LC) were examined by a combination of immunoelectron microscopic mirror method and double-immunostaining method. For visualization of NA and GABA, we used antibodies against NA and GABA synthesizing enzymes, i.e., tyrosine hydroxylase (TH) and glutamic acid decarboxylase (GAD). GAD-immunoreactive (IR) and SP-IR axon terminals often made synaptic contacts with NA neurons, respectively. Furthermore, we identified that a single NA neuron simultaneously receives synaptic inputs from GAD-IR and SP-IR afferents. These NA neurons made symmetrical synaptic contacts with GAD-IR axon terminals and asymmetrical contacts with SP-IR axon terminals. This suggests that central NA neuronal mechanisms are affected by GABA and SP neurons in a different manner.
 ...
PMID:GABA-ergic and substance P-ergic double-innervation to noradrenergic neurons in the rat locus coeruleus. 930 37

The present study determined the effects of intraventricularly administered glial cell line-derived neurotrophic factor on the behavioral and neurochemical sequelae of unilateral excitotoxic lesions of the striatum. Distinct asymmetrical rotational behavior in response to peripheral administration of amphetamine (5 mg/kg) was noted one and two weeks following injections of quinolinic acid (200 nmol) into two sites in the left striatum. In rats given a single intraventricular injection of glial cell line-derived neurotrophic factor (10-1000 micrograms) 30 min before the toxin, amphetamine-induced rotational behavior was significantly attenuated. Analysis of Nissl-stained coronal sections showed marked neuronal loss in the striatum ipsilateral to the quinolinic acid injections, which was at least partially prevented by glial cell line-derived neurotrophic factor D1 and D2 dopamine binding sites in the striatum, the majority of which are localized to subpopulations of GABAergic neurons, were decreased to a similar extent by quinolinic acid. Moreover, the reduction was attenuated by glial cell line-derived neurotrophic factor treatment to a similar degree, suggesting that the two subpopulations of GABAergic striatal output neurons are equally vulnerable to excitotoxic damage. Concomitant changes in neurotransmitter function as a result of the lesion were also observed: [3H]GABA uptake into striatal target tissues (globus pallidus and substantia nigra) was considerably reduced in the lesioned compared to the contralateral unlesioned tissues, as were [3H]choline and [3H]dopamine uptake into striatal synaptosomes. Similarly, striatal choline acetyltransferase activity was decreased by the lesion. Decrements in neuropeptide levels of similar magnitude were evident ipsilateral to the lesion; substance P, met-enkephalin and dynorphin A contents in the globus pallidus and substantia nigra were significantly reduced. Striatal somatostatin and neuropeptide Y levels were not altered. All of the neurochemical deficits induced by striatal quinolinic acid lesions were attenuated by intraventricular delivery of glial cell line-derived neurotrophic factor. Continuous intraventricular infusion of this trophic factor (10 micrograms/day) over a two-week period did not afford notable improvement compared to the single injection of 10 micrograms. In contrast, continuous infusion of brain-derived neurotrophic factor (10 micrograms/day) directly into the striatum did not affect any of the neurochemical parameters studied. However, neurotrophin-3 (10 micrograms/day) delivery into the striatum significantly increased [3H]GABA uptake, but only modestly affected [3H]choline uptake. The results indicate that glial cell line-derived neurotrophic factor counteracts neuronal damage induced by a striatal excitotoxic insult and support its potential use as a treatment for central nervous system disorders that may be a consequence of excitotoxic processes, such as Huntington's disease.
 ...
PMID:Glial cell line-derived neurotrophic factor attenuates the excitotoxin-induced behavioral and neurochemical deficits in a rodent model of Huntington's disease. 933 Mar 71


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>