Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P50583 (asymmetrical)
 12,197 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Mice are commonly used for the study of human disease processes. However, techniques for measuring systemic and hepatic blood flow in mice have not been developed. We attempted the conventional technique of radiolabeled microsphere injection into the left ventricle, but difficulties were encountered, including unsuspected ventricular perforation and outflow obstruction in 68% of the animals. We therefore evaluated whether an injection into the carotid artery close to the aortic arch can fulfill the criteria (approximately or greater than 300 microspheres in femoral blood or tissues, adequate mixing of microspheres with blood, and no significant alteration of blood pressure during microsphere injection) required for accurate measurement of systemic and regional hemodynamics. Carotid artery injection resulted in adequate mixing and number of microspheres in tissues in 78 and 91% of the animals, respectively. Portal venous inflow was 1.8 +/- 0.3 ml.min-1.g liver tissue-1 and renal blood flow was 5.1 +/- 0.75 ml.min-1.g tissue-1. Compared per unit weight, these values are quite similar to those reported in rats. Cardiac output was 12.1 +/- 1.2 ml/min and cardiac index was 462 +/- 47 ml.min-1.kg body wt-1. The reliability of cardiac output determination is improved if whole body radioactivity is taken into account. Five of 22 animals had to be excluded because of either a low number of microspheres in tissues or inadequate mixing of microspheres with blood (shown by asymmetrical distribution of microspheres between left and right kidney). With modifications, the radioactive microsphere technique can be adapted to the hemodynamic study of mice.
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PMID:Splanchnic and systemic hemodynamics in mice using a radioactive microsphere technique. 231 51

Previous studies have shown that Siamese fighting fish, Betta splendens, preferentially use right-eye during the aggressive displays. However, administration of antidepressant drugs may disrupt eye-use preference in association with a reduction in aggression; a phenomena that has not been explored in fish. The objective of the current study was to examine the effects of exposure to the antidepressant drug, fluoxetine, on lateralization in eye-use during aggressive displays in male Siamese fighting fish. Baseline aggression and lateralization in eye use of thirty fish were assessed toward live conspecifics, following which experimental subjects (n=15) were then exposed to fluoxetine (540ng/L) in a static renewal water system. Behavior was quantified again after 9 days of exposure. All of the subjects preferentially used the right-eye during aggressive responses before the exposure experiments. Fluoxetine exposed subjects showed a reduction in the time spent gill flaring as has previously been reported, indicative of a reduction in the level of aggression. Fluoxetine also had a significant effect on the lateralization in preferred eye-use while looking at their opponent. Fish exposed to fluoxetine switched from a preferential use of the right-eye during aggressive encounters prior to exposure to using their left-eye after exposure to fluoxetine. The results are discussed with regard to asymmetrical distribution of serotonin between the two brain hemispheres.
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PMID:Prozac impacts lateralization of aggression in male Siamese fighting fish. 2823 86