Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P50583 (
asymmetrical
)
12,197
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Stem cell specification in multicellular organisms relies on the precise spatiotemporal control of RNA polymerase II (Pol II)-dependent gene transcription, in which the evolutionarily conserved Mediator coactivator complex plays an essential role. In
Arabidopsis thaliana
, SHORTROOT (SHR) and SCARECROW (SCR) orchestrate a transcriptional program that determines the fate and
asymmetrical
divisions of stem cells generating the root ground tissue. The mechanism by which SHR/SCR relays context-specific regulatory signals to the Pol II general transcription machinery is unknown. Here, we report the role of Mediator in controlling the spatiotemporal transcriptional output of SHR/SCR during
asymmetrical
division of stem cells and ground tissue patterning. The Mediator subunit
MED31
interacted with SCR but not SHR. Reduction of
MED31
disrupted the spatiotemporal activation of
CYCLIND6;1
(
CYCD6;1
), leading to defective
asymmetrical
division of stem cells generating ground tissue.
MED31
was recruited to the promoter of
CYCD6;1
in an SCR-dependent manner.
MED31
was involved in the formation of a dynamic
MED31
/SCR/SHR ternary complex through the interface protein SCR. We demonstrate that the relative protein abundance of
MED31
and SHR in different cell types regulates the dynamic formation of the ternary complex, which provides a tunable switch to strictly control the spatiotemporal transcriptional output. This study provides valuable clues to understand the mechanism by which master transcriptional regulators control organ patterning.
...
PMID:Mediator subunit MED31 is required for radial patterning of
Arabidopsis
roots. 2984 59
