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 12,197 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Current voltage (I-V) relations were measured from the calcium release channel (CRC) of the sarcoplasmic reticulum of cardiac muscle in 12 KCl solutions, symmetrical and asymmetrical, from 25 mM to 2 M. I-V curves are nearly linear, in the voltage range +/- 150 mV approximately 12kT/e, even in asymmetrical solutions, e.g., 2 M // 100 mM. It is awkward to describe straight lines as sums of exponentials in a wide range of solutions and potentials, and so traditional barrier models have difficulty fitting this data. Diffusion theories with constant fields predict curvilinear I-V relations, and so they are also unsatisfactory. The Poisson and Nernst-Planck equations (PNP) form a diffusion theory with variable fields. They fit the data by using adjustable parameters for the diffusion constant of each ion and for the effective density of fixed (i.e., permanent) charge P(x) along the channel's "filter" (7-A diameter, 10 A long). If P(x) is described by just one parameter, independent of x (i.e., P(x) = P0 = -4.2 M), the fits are satisfactory (RMS error/RMS current = 6.4/67), and the estimates of diffusion coefficients are reasonable D(K) = 1.3 x 10(-6) cm2/s, D(Cl) = 3.9 x 10(-6) cm2/s. The CRC seems to have a small selectivity filter with a very high density of permanent charge. This may be a design principle of channels specialized for large flux. The Appendix derives barrier models, and their prefactor, from diffusion theories (with variable fields) and argues that barrier models are poor descriptions of CRCs in particular and open channels in general.
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PMID:Permeation through the calcium release channel of cardiac muscle. 928 2

An experimental device consisting of a plane source of charged particles and of a magnetic spectrometer equipped with horizontal and vertical diaphragms is considered. An analysis intended to achieve an exact analytical expression to connect the observed particle flux to the source emission and spectrometer transmission characteristics is developed. An analytical expression for the average value of the source brightness that is actually obtained from corpuscular flux measurements is also given. The effectiveness of the diaphragms in defining the domains of the initial conditions of the particles is also emphasized and general definitions of these domains are introduced. These definitions are stated for exact optical transformations, i.e., accounting for geometric and chromatic aberrations, and for general positions of the diaphragms along the optical axis as well as for symmetrical or asymmetrical apertures of the same. The results obtained and the introduced definitions will be used in Part II of this work. The important case of a thick source is considered in Appendix A.
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PMID:Momentum transmission of a diaphragmed magnetic spectrometer: I. Analysis and definitions. 1869 49