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Query: UNIPROT:P50583 (asymmetrical)
 12,197 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Cytological, histochemical and neurohistological methods were used to study the human retina (27 corpses of people from 60 to 80 years of age). Two retinas from people of 32 and 27 years served as controls. The activity of alkaline phosphatase in neurons was established to increases with age. There were also enlargement of cysts and an increase of the square surface of their distribution in the retina, numerous aneurysmatic swelling along the course of the retinal capillaries. The nerve elements of the retina degenerated more often with age and partly died. Varicosities and excessively growing enlarged terminals were found along the course of dendritic and axonic processes. In the macular zone there were hypertrophicsynapses and circularly running sinuous fine fibres. Solitary ganglionic cells in this region had dendrites directed toward on side-they ere asymmetrical. The astrocytigglia was not changed. The fovea bottom contained may Muller's cells. Possible interpretation of the mechanism of appearance of vaicosities and excrescences in the nerve fibres with age age is presented.
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PMID:[Age characteristics of the structure of the human retina]. 109 21

Serotonin-, substance P-, and thyrotropin-releasing hormone-immunoreactive profiles were studied in the intermediolateral cell column at the thoracic level of the rat spinal cord with light- and electron-microscopic immunocytochemistry. For each transmitter, a dense immunoreactive deposit was observed with the light microscope. At ultrastructural level, morphologically identified synapses amounted to 47% of all serotonergic varicosities, to 49% for substance P and 50% for thyrotropin-releasing hormone. Synapses appeared both symmetrical and asymmetrical. In each case, these synapses were mainly axodendritic (98%). These synaptic connections could mediate the physiological influence of these 3 substances in the spinal cord on the cardiovascular system.
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PMID:5-Hydroxytryptamine, substance P and thyrotropin-releasing hormone synapses in the intermediolateral cell column of the rat thoracic spinal cord. 137 52

Our previous studies have demonstrated the presence of a considerable number of substance P-, calcitonin gene-related peptide (CGRP)-, and galanin-like immunoreactive (LI) nerve fibers in the anterior pituitary in several mammalian species. The present study investigated the ultrastructure of the CGRP-LI innervation of this gland in the dog. The CGRP-LI nerve fibers were unmyelinated, with a wealth of varicosities containing both small clear synaptic vesicles and large dense-cored vesicles. They were found to be in direct contact with every cell type of the anterior pituitary. However, only on corticotropes and somatotropes were CGRP-LI synaptic contacts identified. Most of them were asymmetrical in type. Occasional symmetrical synaptic contacts were also found. It is considered likely that direct neural factors may play a role in the regulation of the anterior pituitary.
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PMID:An electron microscopical study of calcitonin gene-related peptide-like immunoreactive innervation of the anterior pituitary in the dog. 147 63

The distribution and synaptic connections of dopamine axons were studied by light and electron microscopy in human cerebral cortex. For this purpose, dopamine immunoreactivity was characterized in apparently normal anteriolateral temporal cortex, which was removed to gain access to the medial temporal lobe during tumor excision or treatment of epilepsy. Nissl sections showed this to be granular neocortex. Dopamine fibers were distributed throughout this cortex, although there were relatively more fibers in layers I-II and in layers V-VIa, compared to layers III-IV and VIb, resulting in a bilaminar pattern of labeling. In all layers, fibers were seen to form numerous varicosities, and to vary in size from thick to very fine. Fibers were relatively straight, sparsely branched and were oriented in various planes within the cortex. However, in layer I, they often ran parallel to the pial surface. In order to analyze the functional interactions of dopamine fibers, individual cortical layers were surveyed for dopamine synapses. These were usually symmetrical (Gray's type II), although 13% of them were asymmetrical. Approximately 60% of dopamine synapses were made with dendritic spines, and 40% with dendritic shafts, and this ratio was similar in all layers. On both spines and shafts, it was common to see dopamine synapses closely apposed to an unlabeled asymmetric input, suggesting a dopamine modulation of excitatory input. Some postsynaptic dendritic shafts had features of pyramidal cells, including formation of spines. Since pyramidal cells are the major type of cortical spiny neuron, they probably represent the main target of dopamine synapses in this cortex. There were also dopamine profiles apposed to membrane densities on unlabeled axon terminals, suggesting another type of synaptic interaction. These findings provide the first documentation of dopamine synapses in the human cortex, and show that they form classical synaptic junctions. The location of these synapses on spines and distal dendrites, and their proximity to asymmetric synapses, suggest a modulatory role on excitatory input to pyramidal cells.
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PMID:Light and electron microscopic characterization of dopamine-immunoreactive axons in human cerebral cortex. 150 72

This paper deals with the distribution of thyrotropin-releasing hormone-like immunoreactivity in the spinal cord of the rat, and particularly in the sympathetic nuclei, at light and electron microscopic levels. In the dorsal horn, the inner part of laminae II and III displayed thin thyrotropin-releasing hormone immunoreactive profiles. Electron microscopy revealed small immunoreactive varicosities which made synaptic contact with small dendrites or dendritic spines. Dense thyrotropin-releasing hormone-like immunoreactivity was observed in all sympathetic nuclei (nucleus intermediolateralis pars fascicularis and principalis, nucleus intercalatus and dorsal commissural nucleus) except the nucleus intercalatus pars ependymalis. Electron microscopy showed many immunoreactive varicosities which were often in synaptic contact with dendrites (proximal or distal), rarely with perikarya and never with axons. Sometimes, the same immunoreactive varicosity made axodendritic contacts with two dendrites and, conversely one dendrite was sometimes synaptically contacted by two or more immunoreactive varicosities. The ventral horn displayed a diffuse thyrotropin-releasing hormone-like immunoreactivity except for the cremaster nucleus (at lumbar level) which was densely outlined by immunoreactive profiles. Occasionally a large cell body in lamina IX (a putative motoneuron) was outlined by immunoreactive profiles but ultrastructural studies revealed very few immunoreactive axosomatic synapses, while immunoreactive symmetrical or asymmetrical axodendritic synapses were observed. The present study clearly confirms the existence of thyrotropin-releasing hormone immunoreactive synapses, thus substantiating the physiological role of this hormone in the spinal cord.
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PMID:Distribution of thyrotropin-releasing hormone in the rat spinal cord with special reference to sympathetic nuclei: a light- and electron-microscopic immunocytochemical study. 156 Feb 52

Serotonin (5-HT) immunocytochemistry was used at the electron microscopic level to examine 5-HT neurons reinnervating and hyperinnervating the hippocampus of adult rat, three to four months after a total 5-HT denervation and subsequent graft of embryonic raphe cells. The study focused on immunostained nerve cell bodies, dendrites and axon terminals (varicosities) in the core of grafts, and on a large single section sampling of axon terminals from a CA3 and a dentate gyrus sector of the outgrowth, which were systematically compared to the endogenous 5-HT innervation of the same regions described in a companion paper. The shape, size and synaptic investment of the grafted 5-HT somata and their dendrites resembled those of in situ 5-HT neurons. Clusters of small, clear vesicles were sometimes seen along these 5-HT dendrites. 5-HT axonal varicosities were fairly numerous in the core. A few were directly apposed to, or made asymmetrical synaptic contact with the immunostained dendrites and perikarya, but the vast majority showed no indication of junctional specialization (synaptic incidence of 19%, as stereologically extrapolated for whole varicosities). Occasional myelinated 5-HT axons were also present in the core of grafts. In the two outgrowth sectors, the graft-borne 5-HT varicosities were similar in size, content, frequency of synaptic contact and identity of junctional and appositional elements, irrespective of their laminar location. Moreover, none of these parameters were significantly different from those of the endogenous innervation. Notably, in spite of their excessive number, the synaptic incidence of the outgrowth 5-HT varicosities remained inferior to 20%. The similarity between the respective microenvironments of the supernumerary, graft-borne 5-HT terminals and of their normal counterparts could only be explained by a random intratissular distribution of these varicosities in both the normal and the grafted hippocampus. Thus, in spite of their transplantation and growth into an abnormal milieu, and the fact that they hyperinnervated the host tissue, the grafted embryonic 5-HT neurons appeared committed to express a particular set of intrinsic and relational morphological features corresponding to their normal adult characteristics.
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PMID:Ultrastructural features of serotonin neurons grafted to adult rat hippocampus: an immunocytochemical analysis of their cell bodies and axon terminals. 195 16

A combination of electron microscopic immunocytochemistry and autoradiography was employed to examine the synaptic organization of thyrotropin-releasing hormone (TRH) neurons in the periventricular subdivision of the paraventricular nucleus of the rat hypothalamus. TRH neurons were identified by immunocytochemistry. Selective uptake of tritiated serotonin (5-HT) was used to identify serotoninergic elements. TRH-immunoreactive axon terminals were found to be in synaptic contact with TRH-immunoreactive dendrites and with unlabeled dendritic branchlets. There were direct appositions between radiolabeled 5-HT terminals and TRH-immunoreactive dendrites, but differential synaptic contacts between 5-HT axonal elements and TRH neurons were not seen. TRH-immunopositive cell bodies and dendrites received a very intense innervation by unlabeled axon terminals or axonal varicosities showing morphologically defined synaptic junctions. These were mostly of the asymmetrical variety and different types could be distinguished. The findings substantiate the view that TRH neurons of the periventricular subvision of the paraventricular nucleus may be influenced by TRH axons, serotoninergic fibers and a large number of unidentified nerve terminals.
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PMID:Ultrastructural analysis of the innervation of TRH-immunoreactive neuronal elements located in the periventricular subdivision of the paraventricular nucleus of the rat hypothalamus. 212 84

The monoamines dopamine, noradrenaline, adrenaline, and serotonin as well as the diamine histamine have a widespread distribution in the central nervous system within synaptic terminals and nonsynaptic varicosities. In certain regions of the central nervous system the monoamines are contained in varicosities that have no synaptic specialization associated with them, suggesting a possible neuromodulatory role for some of the monoamines. The majority of monoamine labelled structures are synaptic terminals which are characterized by the presence of small, clear vesicles (40-60 nm) and large, granular vesicles (70-120 nm) within the terminal. A third population of vesicles--small, granular vesicles--which are visible only after histochemical staining, are probably the equivalent of the small, clear vesicles present after either autoradiographic or immunohistochemical labelling. Most monoamine containing terminals contact dendrites and dendritic spines and, less frequently, neuronal somata and other axons. Both asymmetrical and symmetrical membrane specializations are associated with monoaminergic terminals; however, asymmetrical contacts are the most frequent type found. These ultrastructural results indicate that monoamine containing terminals and varicosities in general share many common morphological features, but still have diverse functions.
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PMID:Monoamine synaptic structure and localization in the central nervous system. 218 68

Antisera raised against the monoamines serotonin (5-HT) and noradrenaline (NA) were employed in a study designed to provide a detailed description of the distribution, morphology, and synaptic organization of the serotoninergic and noradrenergic afferents in the lateral geniculate nucleus (LGN) of the rat. The distribution patterns of the two types of immunoreactive fibers were distinct and largely complementary to each other. NA axons were particularly concentrated in the dorsal lateral geniculate nucleus (LGd), with the ventral lateral geniculate nucleus (LGv) and the intergeniculate leaflet (IGL) receiving substantially fewer fibers. In contrast, 5-HT axons, although present throughout the LGN, were preferentially concentrated in the LGv and IGL. 5-HT and NA axon terminals and axonal varicosities, examined in single and serial ultrathin sections, formed conventional synapses in the extraglomerular neuropil. The types of synapses and the nature of the postsynaptic targets were different for the two monoamines. 5-HT afferents formed asymmetrical synapses on dendritic spines and shafts of both presumptive relay cells and interneurons but established symmetrical synapses on cell bodies. However, NA afferents formed almost exclusively symmetrical synapses on dendritic spines and shafts and made no contacts with cell bodies. The present findings suggest that the 5-HT and NA afferents of the rat LGN, which are likely to influence certain stages of visual processing, exhibit distinct organizational principles and act at restricted sites as do other classical neurotransmitter systems.
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PMID:Distribution and synaptic organization of serotoninergic and noradrenergic axons in the lateral geniculate nucleus of the rat. 234 14

In the present study, immunocytochemistry with an antiserum against dopamine (DA) revealed hitherto unknown terminal fields of DA axons in the lateral geniculate nucleus (LGN) of the rat. The innervation of all subdivisions of the LGN is achieved by a common set of afferent fibers that branch to form terminal fields of uneven density. The ventral lateral geniculate nucleus (LGv) receives slightly more DA axons than the dorsal lateral geniculate nucleus (LGd), whereas within the latter, DA afferents innervate the lateral part of the nucleus slightly more densely. Labeled axon terminals and varicosities, examined in single and serial ultrathin sections, were found in the extraglomerular neuropil in the LGd and in the neuropil of the LGv characterized by relatively simple synaptic relationships. They formed predominantly asymmetrical synaptic contacts with dendritic profiles. Occasionally, the postsynaptic elements were found to be presynaptic dendrites of presumptive interneurons. Some of the possible roles of this newly demonstrated DA afferent system in the physiology of the LGN and in the pathophysiology of diseases associated with impairment of dopaminergic activity are discussed.
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PMID:Distribution and synaptic organization of dopaminergic axons in the lateral geniculate nucleus of the rat. 234 15


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