Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P50583 (asymmetrical)
 12,197 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

I(H) pacemaker channels carry a mixed monovalent cation current that, under physiological ion gradients, reverses at approximately -34 mV, reflecting a 4:1 selectivity for K over Na. However, I(H) channels display anomalous behavior with respect to permeant ions such that (a) open channels do not exhibit the outward rectification anticipated assuming independence; (b) gating and selectivity are sensitive to the identity and concentrations of externally presented permeant ions; (c) the channels' ability to carry an inward Na current requires the presence of external K even though K is a minor charge carrier at negative voltages. Here we show that open HCN channels (the hyperpolarization-activated, cyclic nucleotide sensitive pore forming subunits of I(H)) undergo a fast, voltage-dependent block by intracellular Mg in a manner that suggests the ion binds close to, or within, the selectivity filter. Eliminating internal divalent ion block reveals that (a) the K dependence of conduction is mediated via K occupancy of site(s) within the pore and that asymmetrical occupancy and/or coupling of these sites to flux further shapes ion flow, and (b) the kinetics of equilibration between K-vacant and K-occupied states of the pore (10-20 micros or faster) is close to the ion transit time when the pore is occupied by K alone ( approximately 0.5-3 micros), a finding that indicates that either ion:ion repulsion involving Na is adequate to support flux (albeit at a rate below our detection threshold) and/or the pore undergoes rapid, permeant ion-sensitive equilibration between nonconducting and conducting configurations. Biophysically, further exploration of the Mg site and of interactions of Na and K within the pore will tell us much about the architecture and operation of this unusual pore. Physiologically, these results suggest ways in which "slow" pacemaker channels may contribute dynamically to the shaping of fast processes such as Na-K or Ca action potentials.
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PMID:Ion binding in the open HCN pacemaker channel pore: fast mechanisms to shape "slow" channels. 1827 Jan 71

The plane-wave theory for the transmittance and absorbtance of a perfectly aligned Michelson coupler with a dielectric slab beam splitter is presented. It is shown that the transmittance and absorbtance vary sinusoidally and in quadrature. As a result of this quadrature relationship, the maximum transmittance occurs at a setting of the translatable coupler mirror at which the absorbtance is not at an extremum, and so the curve of output power as a function of coupler setting is asymmetrical with respect to the setting yielding maximum transmittance. Experimental measurements of the output power of a far-infrared HCN laser as a function of the coupler setting confirm this asymmetry, which seems to have been overlooked or ignored in previous studies.
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PMID:Plane-wave theory of a Michelson laser coupler with a dielectric slab beam splitter. 2108 49