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Query: UNIPROT:P50502 (Hip)
 7,003 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. The aim of the present study was to determine the effect of nitric oxide (NO) on angiotensin-converting enzyme (ACE) activity. 2. A biochemical study was performed in order to analyse the effect of the NO-donors, SIN-1 and diethylamine/NO (DEA/NO), and of an aqueous solution of nitric oxide on the ACE activity in plasma from 3-month old male Sprague-Dawley rats and on ACE purified from rabbit lung. SIN-1 significantly inhibited the activity of both enzymes in a concentration-dependent way between 1 and 100 microM. DEA/NO inhibited the activity of purified ACE from 0.1 microM to 10 microM and plasma ACE, with a lower potency, between 1 and 100 microM. An aqueous solution of NO (100 and 150 microM) also inhibited significantly the activity of both enzymes. Lineweaver-Burk plots indicated an apparent competitive inhibition of Hip-His-Leu hydrolysis by NO-donors. 3. Modulation of ACE activity by NO was also assessed in the rat carotid artery by comparing contractions elicited by angiotensin I (AI) and AII. Concentration-response curves to both peptides were performed in arteries with endothelium in the presence of the guanylyl cyclase inhibitor, ODQ (10 microM), and the inhibitor of NO formation, L-NAME (0.1 mM). NO, which is still released from endothelium in the presence of 10 microM ODQ, elicited a significant inhibition of AI contractions at low concentrations (1 and 5 nM). In the absence of endothelium, 1 microM SIN-1 plus 10 microM ODQ, as well as 10 microM DEA/NO plus 10 microM ODQ induced a significant inhibition on AI-induced contractions at 1 and 5 nM and at 1-100 nM, respectively. 4. In conclusion, we demonstrated that (i) NO and NO-releasing compounds inhibit ACE activity in a concentration-dependent and competitive way and that (ii) NO release from endothelium physiologically reduces conversion of AI to AII.
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PMID:Modulation of angiotensin-converting enzyme by nitric oxide. 964 45

Current classification systems used for developmental dysplasia of the hip (DDH) in adult patients about to undergo total hip arthroplasty use a combined assessment of the acetabulum and femur which ignores femoral geometry and problems related to reconstruction of leg length. Currently accepted systems are those of Crowe and Hartofilakidis; it is our opinion that they do not predict surgical difficulties encountered at total hip arthroplasty. We describe a new classification system for adult DDH that divides the acetabulum and femur into separate components. The acetabular classification comprises: AI: Dysplastic acetabulum; AII: The acetabulum associated with a low femoral dislocation; AIII: The post-surgical acetabulum, with (AIIIa) or without retained metalwork (AIIIb). The femoral classification consists of: FI: Dysplastic femur but contained within true or low acetabulum; FII: The high femur; FIII: Post-surgical femur, again with or without metalwork (FIIIa and FIIIb). 50 pre-operative radiographs of hips with DDH about to undergo total hip replacement were assessed by orthopaedic consultants, registrars and medical students. They were classified using the new system, Crowe and Hartofilakidis systems. Interobserver and intraobserever reliability was assessed using Fleiss' kappa coefficient with combined (acetabulum and femur) kappa scores for the new system of 0.69 for interobserver and 0.67 for intraobserver reliability. This equated to 'substantial agreement' according to Landis and Koch and the new system showed at least comparable levels of reliability to the Hartofilakidis and Crowe systems. We have demonstrated reproducibility of our new classification system for DDH in the adult population and believe it could provide useful information when planning arthroplasty and be used to predict technical difficulties and outcome.
Hip Int
PMID:A new classification system for the adult dysplastic hip requiring total hip arthroplasty: a reliability study. 1946 64