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Query: UNIPROT:P50502 (Hip)
 7,003 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The time course of dipeptidase activity and the effect of p-mercuribenzoate (PCMB) on the subestimation of the fluorometric determination of angiotensin-converting enzyme (ACE, EC 3.4.15.1) during development was studied. ACE and dipeptidase activities were measured fluorometrically in homogenates of the developing chick retina using Hip-His-Leu and His-Leu as substrates, respectively, both either in the presence or in the absence of 1 mM PCMB. ACE activity was inhibited by captopril (IC50 1.7 nM), MK 422 (IC50 4.8 nM), BPP9a (IC50 0.25 microM) and BPP5a (IC50 1.2 microM), thus suggesting that avian retinal ACE catalytically resembles the mammalian enzyme. Dipeptidase activity varied 3.4-fold throughout development, leading to a large and variable (28-83%) subestimation of ACE activity during chick retina ontogenesis. PCMB (1 mM) inhibited 67-94% dipeptidase activity during development, thus greatly reducing any subestimation of ACE activity determination during the development of the chick retina.
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PMID:Effect of p-mercuribenzoate on the subestimation of angiotensin-converting enzyme measurement during chick retina development. 215 60

The most sensitive nonradiometric routine assay for angiotensin-converting enzyme (ACE) activity uses fluorometry to detect His-Leu released from Hip-His-Leu. Our results indicate that, in contrast to human serum, rat serum and plasma contain large and variable amounts of dipeptidase activity that lead to a subestimation of the ACE activity measured in 0.1 M potassium phosphate buffer, pH 8.3, containing 0.3 M NaCl, the most commonly used assay for human serum and tissue ACE. We describe and validate an assay for 1 to 10 microL rat and human serum or plasma using 5 mM Hip-His-Leu in 500 microL of 0.4 M sodium borate buffer, pH 8.3, containing 0.9 M NaC1 at 37 degrees C that reduced the subestimation error to less than or equal to 3% (rat serum) and less than or equal to 0.1% (human serum) and increased the ACE activity twofold to threefold. The Km and Vmax are reported for rat serum ACE (Hip-His-Leu) and dipeptidase (His-Leu) in borate buffer and phosphate buffer. Rat serum ACE hydrolysis of Hip-His-Leu measured by fluorometry correlated (r = 0.99, p less than 0.05) with the hydrolysis of angiotensin I measured by high-performance liquid chromatography. A direct method based on amino acid analysis is described for evaluating the dipeptidase error of complex mixtures such as tissue extracts and other physiological fluids. We have found that the assay can be used to measure ACE activity in 25 samples (in duplicate) in 2 hours with small intraassay (2.2%) and interassay (3.9%) coefficients of variation.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:An improved fluorometric assay of rat serum and plasma converting enzyme. 298 18

In eight undisplaced intracapsular fractures of the femoral neck, an intracapsular hematoma was diagnosed by computed tomography. 99mTc-MDP scintimetry revealed markedly reduced or absent blood supply to the head of femur. The intracapsular pressure was 23 (2.7-43) kPa with the hip in neutral position. Following aspiration of 12 (0.5-36) ml of blood, pressure was reduced to zero, and postaspiration scintimetry revealed restitution of blood supply to the femoral head. Hip joint tamponade in these patients has caused femoral head ischemia, reversible by aspiration.
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PMID:Hemarthrosis in undisplaced cervical fractures. Tamponade may cause reversible femoral head ischemia. 378 90

In 84 patients with an intracapsular hip fracture, early technetium-99m methylene diphosphonate (99mTc-MDP) scintimetry could be carried out preoperatively of in the case of conservative treatments within 72 h after injury. Fifty-four patients were available for a 3-year follow-up. The corrected uptake ratio showed a very high accuracy in predicting secondary dislocation, non-union (NU), delayed union (DU) or femoral head necrosis (FHN) and was far more reliable than the uncorrected uptake ratio. The predictive value of scintimetric follow-up at a 2- or 12-week interval could not reach the same accuracy of early scintimetry in predicting poor outcome. A comparison of follow-up scintimetry after conservative treatment and osteosynthesis showed that the major vascular injury to the femoral head is caused at the moment of the initial trauma and that no additional damage occurs perioperatively with closed reduction and fixation with a Dynamic Hip Screw device. Scintimetry is a useful tool in predicting poor outcome due to FHN and/or NU in the treatment of intracapsular hip fractures.
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PMID:Predictive value of early scintimetry in intracapsular hip fractures. A prospective study with regard to femoral head necrosis, delayed union and non-union. 811 8

In this prospective study the authors analysed the prognostic value of monitoring patency of the feedings vessels of the fibular graft, implanted into the femoral head to treat aseptic necrosis. In 26 patients (29 hips) we applied a three phase 99Tc-MDP Bone Scanning in the seventh postoperative day. One week later; 18 patients had a digital subtraction angiography of the lateral femoral circumflex artery and its branches. The patients were followed for average time of 22 months. The average Harris Hip Score of the affected hips improved clinically from 69.5 to 93.5. The vessels were patent prior to the wound closure. Phase I of the bone scan served as "radionuclide angiogram" and demonstrated patent vessels in all about three hips. The digital arteriograms demonstrated patent anastomotic sites in 15 out of 18 hips. Only in four of the fifteen arteriograms the vessels were visualized throughout the length of the graft. Two of the three hips with negative arteriograms have also had negative bone scans, both in patients with haemoglobinopathies. None of the patients required further surgery because of failure of the procedure. This study demonstrated that the phase I of the bone scan at the end of the first week postoperatively is very sensitive means for monitoring the viability of the graft. Digital arteriograms although more specific, were less sensitive. Early postoperative monitoring of the graft viability maintained high predictive value for the outcome of the treatment in the medium-term.
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PMID:Three phase bone scanning and digital arteriograms for monitoring vascularized fibular grafts in femoral head necrosis. 891 54

We present the clinical and radiological results of a prospective study of a consecutive series of patients treated with the Birmingham Hip Resurfacing (BHR) prosthesis between 1999 and 2006 and followed for six to 12 years. Patients were reviewed preoperatively and six, 12, 26 and 52 weeks postoperatively and annually thereafter. They were assessed clinically and radiographically and the Merle d'Aubigne Postel and Oxford Hip scores were calculated at each visit. A SF-36 form assessed general health. Patient satisfaction was assessed by a Visual Analogue Score. Kaplan Meier survival analysis was performed.One hundred and seven patients (109 hips) were included (45 males, 62 females). Median age was 44 years. Mean follow-up was 97.4 months. Median preoperative Merle d'Aubigne Postel and Oxford hip scores were 10 (3-14) and 42 (26-55) respectively. Median SF36 score was 29 (0-65) and patients rated their level of pain as 7.7 on a scale of 0-10 (10 being the worst score). At final follow-up, median MDP and OHS scores were 17 (16-18) and 25 (12-46). Median SF36 score was 72 (27-97). Seven revisions were performed in six females and one male patient. Survival was 96.2% with revision for any reason as the end point. No revisions were performed in the group with hip dysplasia.The BHR prosthesis provides pain relief, high levels of patient satisfaction and improvement of function and well being in the medium-term. Failures occurred primarily in the female group.
Hip Int
PMID:Patient satisfaction and clinical results at a mean eight years following BHR arthroplasty: results from a district general hospital. 2481 95