Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P47989 (xanthine oxidase)
 8,633 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Oren-Gedoku-To (TJ-15) inhibited enzymatically (NADPH or CumOOH) and non-enzymatically (Fe-ascorbate) induced lipid peroxidation in rat liver microsomes as assessed by TBA-reactive product accumulation. Toki-Shakuyaku-San (TJ-23) had little effect on either system. The protective effect of TJ-15 against lipid peroxidation was not dependent upon the presence of microsomal drug-metabolizing activity and could not be fully accounted for by its action on microsomal electron transfer, as evaluated by studying the kinetics of cytochrome c reduction. Both TJ-15 and TJ-23 reduced the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), indicating their free radical scavenging ability. The reactivity of TJ-15 was comparable with that of standard ascorbate, while the activity of TJ-23 was approximately 10 times lower. In a free solution TJ-15 and TJ-23 effectively scavenged OH* radicals, as indicated by the inhibition of ethylene production from 2-keto-4-methiolbutyric acid (KMBA), and O2*- anion radicals, as assessed by the inhibitory effect on the rate of nitro blue tetrazolium (NBT) reduction by the O2*- generating system xanthine/xanthine oxidase.
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PMID:In vitro studies on the activity of japanese kampo herbal medicines Oren-Gedoku-To (TJ-15) and Toki-Shakuyaku-San (TJ-23) as scavengers of free radicals. 1236 92

Dang-Gui-Shao-Yao-San (DGSYS) is a mixture of medicinal herbs, which has long been used in traditional Chinese medicine for treating anemia and ovulary disorders. Its preparation comprises Angelicae sinensis (Oliv.) Diels, Ligustucum chuanxiong Hort, Paeonia lactiflora pall, Poria cocos (Schw.) Wolf, Atractylodis macrocephala Koidz and Alisma orientalis (Sam.) Juzep. The present study examined the anti-superoxide formation, free radical scavenging and anti-lipid peroxidation activities of DGSYS by xanthine oxidase inhibition, cytochrome C system with superoxide anion released by the fMLP or PMA activating pathway in human neutrophils, and FeCl2 ascorbic acid-induced lipid peroxidation effects on lipids in rat liver homogenate, respectively. DGSYS showed anti-superoxide formation and free radical scavenging activity in a concentration-dependent manner. It also inhibited PMA- but not fMLP-induced superoxide anion released from human neutrophils. These antioxidant actions of DGSYS showed beneficial cytoprotective effects against lipid peroxidation in rat liver homogenate, human platelet aggregation induced by arachidonic acid (AA) and adenosine diphosphate (ADP) and mitomycin C-mediated hemolytic in human erythrocytes.
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PMID:Antioxidant and antiplatelet effects of dang-gui-shao-yao-san on human blood cells. 1626 87