Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P47989 (xanthine oxidase)
 8,633 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The inhibition of the proliferation rate of the immortalized human cell line ECV 304 after oxidant damage by oxygen radicals generated in a hypoxanthin-xanthine oxidase system and the protection provided by various propolis extracts was determined. Best inhibition was demonstrated by 60-80% ethanolic extracts (IC50 of approximately 2 microg dry weight/ml) and by the ethyl acetate extract (IC50 of 6.9 microg dry weight/ml). The beneficial effect of polar extracts was quite weak. Human neutrophil elastase activity was inhibited distinctively by ethanolic (60 to 96%) and ethyl acetate extracts (IC50 of approximately 2 microg dry weight/ml). Both activities seem to be responsible for the anti-inflammatory effect of the extract.
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PMID:Effects of propolis on hypoxanthine-xanthine oxidase-induced toxicity in cultivated human cells and on neutrophil elastase activity. 1599 94

The aim of this study was to investigate the mechanism for the increase in endothelial permeability induced by human neutrophil elastase (HNE). Pretreatment of bovine pulmonary artery endothelial cells (BPAEC) with HNE(0-30 mug/ml) for 1 h produced a concentration dependent increase in (125)I-albumin clearance. The effect was reversible and was not due to cytolysis. Pretreatment of BPAEC with sodium tungstate, which depletes xanthine oxidase, or with oxypurinol, did not prevent HNE induced increased permeability. Heparin, which neutralizes the cationic charge of HNE, also had no protective effect. Pretreatment with heat inactivated HNE, which still had positive charge sites, did not result in increased endothelial permeability. Also, ONO-5046, a novel specific inhibitor of HNE, did prevent increased permeability. These results suggest that elastase increases endothelial permeability mainly through its proteolytic effects.
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PMID:Mechanism for the increased permeability in endothelial monolayers induced by elastase. 1847 17


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