Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P47989 (xanthine oxidase)
 8,633 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The XOR activity in human plasma was measured by quantifying the XOR-derived uric acid (UA) in plasma using the high-performance liquid chromatography (HPLC) equipped with a UV detector. Chromatographic separation consisted of the mobile phase (a mixture of 0.1% trifluoroacetic acid in Milli-Q water and 0.085% trifluoroacetic acid in acetonitrile in a mix ratio of 99:1) running through a Zorbax StableBond SB-C(18) column at a flow-rate of 1 ml/min. Deproteinization with heat-treatment of plasma samples after the reaction was used in the assay to avoid splitting of the UA and xanthine peaks caused by acid deproteinization that could interfere the accurate determination of human plasma XOR activity in our case. Based on the examination of the dependence of XOR activity on added amounts of xanthine and reaction times, the amount of xanthine and reaction time for XOR activity assay were determined to prevent the errors caused by the limiting effect of substrates and plateau phase of the reaction. Using this method, human plasma XOR activities of 25 healthy people were measured. The average human plasma XOR activity was 2.1+/-0.8 (x10(-3) U/ml).
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PMID:Improved method for measurement of human plasma xanthine oxidoreductase activity. 1253 43

A type of tunable femtosecond soliton logic gate based on fiber Raman Self-Frequency Shift (SFS) is studied in this paper. The Raman SFSs of femtosecond solitons governed by the Newton's cradle mechanism in logic gate are analyzed with an Improved Split-Step Fast Fourier Transform (ISSFFT) algorithm. The impact factors of the solitonic pulse frequency shift and temporal time shift, which are included the Third-Order Dispersion (TOD) effect, are investigated. The existing theoretical equation of SFS is modified into a new expression for this type of soliton logic gate. A lower switching power and the small size of the soliton logic gate device is designed to realize the logic functions of AND, NOT, and XOR. The results demonstrate that the logic gate based on SFS is belonged to the asynchronous system and can be achieved with Milli-Watt switching power and good extinction ratio. ISSFFT is effective and accurately to analyze higher-order dispersive and nonlinear effects in the logic gates.
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PMID:The analysis of all-optical logic gates based with tunable femtosecond soliton self-frequency shift. 2471 9