Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P47989 (xanthine oxidase)
 8,633 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Atherogenic lipoproteins accumulate in the arterial wall and may potentially stimulate neighboring cells. In the glomerulus the vascular pole resembles afferent arteries in close vicinity to the juxtaglomerular apparatus. We examined the effects of native and oxidized LDL and lipoprotein(a) [Lp(a)] on renin release of juxtaglomerular cells (JG cells) prepared in primary culture from mouse kidneys. Renin activity of JG cells was measured in culture supernatants and cells between the 20th and 40th hour of culturing. Spontaneous renin release into the cell supernatant was 26 +/- 1% of total activity. Control stimulation of JG cells by melittin or forskolin dose-dependently increased renin release up to 90 +/- 2%. Incubation of JG cells with native LDL (50 and 300 micrograms/ml) or native Lp(a) (30 micrograms/ml) did not alter renin release. Oxidized LDL increased renin release to 34 +/- 1% and 43 +/- 1% at 50 and 300 micrograms/ml, while oxidized Lp(a) stimulated renin release to 33 +/- 1%, 42 +/- 1%, and 71 +/- 2% at 1, 10, and 30 micrograms/ml, respectively. Coincubation with superoxide dismutase and catalase, enzymes removing O2- and H2O2, completely eliminated oxidized LDL and Lp(a)-stimulated renin release. In the absence of lipoproteins, renin release was significantly stimulated by activation of O2- formation by the xanthine/xanthine oxidase reaction. These data indicate that oxidized LDL and Lp(a) stimulate renin release in JG cells by a mechanism involving oxygen-derived radicals. Thus, oxidatively modified atherogenic lipoproteins may contribute to renin-dependent hypertension in renoparenchymatous kidney disease.
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PMID:Oxidized LDL and lipoprotein(a) stimulate renin release of juxtaglomerular cells. 773 Nov 69

We assessed effects of reactive oxygen metabolites on renin release of juxtaglomerular cells (JG-cells) prepared in primary culture from mouse kidneys. Renin activity was measured in culture supernatants and cells. Basal renin release was increased by incubation of JG-cells with xanthine/xanthine oxidase from 26 +/- 1% up to 58 +/- 3% of total activity. This increase was slightly inhibited by superoxide dismutase, and was eliminated by addition of catalase, implicating H2O2 as an intermediate product in the stimulatory cascade. H2O2 applied exogenously dose-dependently stimulated renin release up to 55 +/- 2%; this effect was also prevented by catalase. We propose that reactive oxygen metabolites stimulate renin release in isolated JG-cells. This could have important implications in inflammatory kidney diseases.
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PMID:Oxygen-derived radicals stimulate renin release of isolated juxtaglomerular cells. 808 86