Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P43146 (
tumour suppressor
)
5,935
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
COM1
(candidate of metastasis 1) has been recently shown to influence the metastatic ability of cancer cells and disease progression of certain solid tumours. The role of
COM1
in bladder cancer remains unknown. In the present study, we examined the expression of the
COM1
protein in human bladder tissues, and also its effect on growth, adhesion, migration and invasion of human bladder cancer cells, in vitro. The expression of
COM1
in human bladder tissues and bladder cancer cell lines was assessed at both the mRNA and protein levels using RT-PCR and immunohistochemistry, respectively.
COM1
staining was compared with tumour staging. Mammalian
COM1
expression construct and anti-
COM1
ribozyme transgenes were used to generate sublines of human bladder cancer cells with differential expression of
COM1
. The effect of
COM1
on cellular functions was examined in bladder cancer cells with which
COM1
was overexpressed or knocked down using a variety of in vitro assays. In normal bladder tissues, stronger staining of
COM1
was seen in the cytoplasm of normal urothelial cells. In contrast, the staining was notably weak or absent in cancer cells of tumour tissues and invasive tumours had significantly low levels of staining compared with non-invasive tumours (p=0.012). Knockdown of
COM1
in bladder cancer cell lines resulted in an increase in cellular growth and invasion, while overexpression of
COM1
suppressed invasiveness and growth of these cells. Further investigation revealed an increased apoptosis and upregulated p21 in bladder cancer cells when
COM1
was overexpressed.
COM1
is expressed at low levels in human bladder cancer and in particular in invasive bladder tumours.
COM1
levels are inversely correlated with the invasiveness and growth of bladder cancer cells in vitro. Induced apoptosis and upregulation of p21 are indicated in the mechanism of
COM1
inhibiting bladder cancer cell growth. It suggests that
COM1
is a potential
tumour suppressor
in human bladder cancer.
...
PMID:Candidate of metastasis 1 regulates in vitro growth and invasion of bladder cancer cells. 2344 4
