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Query: UNIPROT:P43026 (lipopolysaccharide)
 62,215 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effect of Pseudomonas aeruginosa infection on contact sensitivity to 2-phenyl-4-ethoximethylene-oxazolone (oxazolone) and on antibody response to sheep erythrocytes, horse erythrocytes, and Escherichia coli 0111:B4 lipopolysacharide was investigated in outbred C57BL/6 mice. Injection of 0.5 and 0.2 median lethal doses significantly depressed contact sensitivity to oxazolone, whereas injection of 0.5 median lethal dose of heat-killed microorganisms did not. The filtrate of a 24-h broth culture did not affect contact sensitivity as well. Antibody production against sheep erythrocytes, horse erythrocytes, and lipopolysaccharide (evaluated as plaque-forming cells and circulating hemagglutinin and hemolysin titers) was found to be significantly enhanced both in animals injected with living bacteria and in those which received heat-killed microorganisms. The simultaneous occurrence of depression of cell-mediated immunity and potentiation of humoral response suggests that P. aeruginosa might interfere at different levels of the host immunological responsiveness.
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PMID:Depression of contact sensitivity and enhancement of antibody response in Pseudomonas aeruginosa-infected mice. 81 24

Rough mutants of two strains of Pseudomonas aeruginosa have been isolated by selection for resistance to virulent lipopolysaccharide-specific phages. The rough mutants fell into two classes on the basis of colonial morphology and agglutination by acriflavine and NaCl. The pigment and exoenzyme-synthesizing properties of these derivatives were identical with those of the parental cultures. The rough strains were 8- to 62-fold less pathogenic for the larvae of the wax moth (Galleria mellonella) than the smooth wild-type cells.
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PMID:The pathogenicity of rough strains of Pseudomonas aeruginosa for Galleria mellonella. 81 78

In the present study Pseudomonas aeruginosa lipopolysaccharide (LPS) exhibited the following endotoxin properties: (1) toxicity for mice; (2) gelation of the Limulus lysate; (3) induction of a localized Shwartzman reaction in the skin of rabbits, and (4) anticomplementary activity. Differences in LPS toxicity as measured with the rat liver mitochondrial assay system were found to be related to the nature of the bacterial growth media, the functional integrity of mitochondria, and the time and temperature of mitochondrial assay. The significance of these findings to P. aeruginosa infections is discussed, and it is concluded that LPS is a factor of importance.
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PMID:Pseudomonas aeruginosa lipopolysaccharide: factors influencing toxicity for isolated mitochondria and endotoxin properties. 81 17

Specific passive immunity against Pseudomonas aeruginosa sepsis was assessed in granulocytopenic dogs. Dogs were infused with either normal or antipseudomonas immune plasma 24 h before pseudomonas challenge. They were challenged intravenously with 10(7) serotype 6 P. aeruginosa during granulocytopenia. Treatment was evaluated by observation of survival periods, febrile responses, type 6 pseudomonas antibody titers, and quantitative cultures of blood and tissues. The results demonstrated that passively immunized dogs did not survive infection. Both normal-plasma and immune-plasma recipients had bacteremia at death, with median values of 980 and 470 pseudomonas per ml of blood, respectively. All dogs had marked febrile responses 24 h after pseudomonas challenge and had high concentrations of pseudomonas in their lung tissue at death, with median values of 10(8) pseudomonas per g of wet tissue weight. After plasma infusion, immune-plasma recipients had high concentrations of anti-pseudomonas antibody, with total antibody titers ranging from 256 to 1,024 and a median value of 1,024. These titers were comparable to titers attained in a previous study from our laboratory using active immunization with pseudomonas lipopolysaccharide vaccine, where the median total anti-pseudomonas antibody titer was 2,048. Actively immunized animals, however, were significantly protected against pseudomonas sepsis and had prolonged survival periods and prevention of bacteremia. The present study demonstrates that circulating type-specific antibody is not solely responsible for the protection afforded to granulocytopenic dogs actively immunized against pseudomonas.
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PMID:Passive immunity against pseudomonas sepsis during granulocytopenia. 82 5

The isolation and some properties of a lipopolysaccharide (LPS)-specific phage isolated against Pseudomonas aeruginosa is reported. The phage, designtaed omega PLS-I, is a Bradley A type phage with a head diameter of 70 nm and a contractile tail 120 nm long. The average adsorption rate constant for omegaPLS-I is 4-48 X 10(-9) ml/min. omegaPLS-I is inactivated by purified LPS from P. aeruginosa strain 1-1A, showing a PhI50 value of 1-25 mug/ml.
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PMID:The isolation and characterization of a lipopolysaccharide-specific Pseudomonas aeruginosa bacteriophage. 82 3

The authors elaborated a method of obtaining a highly active lipopolysaccharide preparation from the cell membranes of the Pseudomonas aeruginosa whose sensitizing activity exceeded that of the polysaccharide isolated from the intact microbial cells almost 20 times. The optimal sensitizing dose of the preparation constituted 15 micron/ml. An erythrocytic diagnostic agent permitting to determine the antibodies in the sera of patients with the purulent-in flammatory diseases caused by Ps. aeruginosa was prepared on the basis of the purified lipo polysaccharide. A definite dependence of the hemagglutinin titres on the severity of the affection and the degree of the purulent-inflammatory process was revealed.
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PMID:[Immunologic study of the cellular components of pseudomonas pyocyaneus. II. Extraction of purified lipopolysaccharide, its characteristics and elaboration of an erythrocyte O-diagnosticum]. 82 24

3-O-Methyl-L-xylose was isolated from whole cells of Pseudomonas maltophilia N.C.T.C. 10257. The sugar is a component of lipopolysaccharide from which a polysaccharide also containing L-rhamnose and L-xylose was released by mild acid hydrolysis. 3-O-Methyl-L-xylose was absent from five other strains of Ps. maltophilia and one strain of Pseudomonas geniculata.
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PMID:Presence of 3-O-methyl-l-xylose in the lipopolysaccharide of Pseudomonas maltophilia N.C.T.C. 10257. 86 17

Sublethal doses of vincristine (VNC) and bacterial lipopolysaccharide (LPS) administered simultaneously to adult male mice resulted in markedly enhanced mortality. All of 10 strains of Pseudomonas aeruginosa tested, 4 of 7 strains of Bacteroides, and 6 of 10 strains of Listeria monocytogenes were able to substitute for purified LPS in enhancing mortality in VNC-treated mice. Inoculation of mice with each of 10 strains of Pseudomonas, each of 7 strains of Bacteroides, and about half of the 10 strains of Listeria tested elicited increased resistance to the lethal action of purified LPS. The patterns of responses of mice receiving a lethal combination of 2 mg of LPS/kg and 1 mg of VNC/kg resembled those of mice receiving a lethal dose of 10 mg of VNC/kg alone or 15 mg of LPS/kg alone with respect to (i) serum glutamic pyruvate transaminase activity, (ii) hematocrit values, and (iii) thrombocytopenia. The patterns of responses of mice receiving a lethal combination of LPS and VNC resembled those of mice receiving a lethal dose of LPS alone with respect to (i) hypothermia, (ii) retention of sulfobromophthalein, (iii) fibrinogen level, (iv) prothrombin activity, (v) blood urea nitrogen levels, and (vi) time of death. These data are consistent with the proposition that the combination of VNC and LPS produces a fatal renal failure. Histological studies confirmed that there was extensive renal damage in mice treated with lethal doses of LPS alone or a lethal combination of LPS and VNC.
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PMID:Enhanced toxicity for mice of combinations of bacterial lipopolysaccharide and vincristine. 94 80

The influence of pentosanepolysulfate (SP 54) and a combination of pentosanepolysulfate, metamizol and paracetamol (Probaphen) on the course of an experimentally induced erythema caused by intracutaneous injection of a lipopolysaccharide from Pseudomonas has been studied in man. The intramuscular injection of Probaphen as well as SP 54, separately given, showed a significant antiinflammatory effect. A similar effect was also achieved after administration of Probaphen suppositories. Probaphen shortened the inflammatory reaction more effectively than did SP 54 alone. The oral application of Probaphen, however, did not influence the course of inflammation.
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PMID:[Studies on the influence of pentosanepolysulfate and a combination of pentosanepolysulfate, metamizol and paracetamol on inflammatory liability (author's transl)]. 110 8

As a step toward developing recombinant oral vaccines, we have explored the feasibility of expression of O polysaccharide antigens from Pseudomonas aeruginosa by Escherichia coli. We cloned in E. coli HB101 a 26.2-kilobase DNA fragment from P. aeruginosa strain PA103 that specifies the production of the O polysaccharide of Fisher immunotype 2 (IT-2) strains. The recombinant organism incorporated the P. aeruginosa IT-2 O polysaccharide onto the core of the E. coli lipopolysaccharide (LPS). Transfer of the recombinant plasmid to three LPS-rough strains of P. aeruginosa resulted in synthesis of IT-2 O antigen, and two of these transconjugant strains also synthesized a second O polysaccharide, presumably representing expression of a repressed, or an incomplete, set of genes for an endogenous O polysaccharide. Rabbits injected with the purified recombinant LPS made antibody specific for P. aeruginosa IT-2 O side chains, as did mice fed the recombinant E. coli strain. Expression of P. aeruginosa O antigens by enteric bacteria makes it possible to study these recombinant strains as oral vaccines to prevent P. aeruginosa infections.
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PMID:Cloning and surface expression of Pseudomonas aeruginosa O antigen in Escherichia coli. 127 92


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