Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P43026 (lipopolysaccharide)
 62,215 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The non-specific cell receptor protein (NCCRP-1) serves an important function in target cell recognition and activation of non-specific cytotoxic cells in teleosts. Atlantic cod NCCRP-1 was identified in a suppression-subtractive cDNA library and NCCRP-1 from Atlantic salmon, rainbow trout, Japanese medaka and fathead minnow was found deposited in the GenBank as EST sequences. The predicted amino acid sequences of these receptors contain the characteristic functional domains representing NCCRP-1, and phylogenetic analyses support the identification of five NCCRP-1 orthologues. Cod NCCRP-1 is shorter and has a different intron/exon organization from the common carp and channel catfish counterparts, but shows high extent of conservation in NCCRP-1 signature motives. Quantitative real-time PCR analyses showed that the gene expression of cod NCCRP-1 was higher in the lymphoid organs, head kidney (90-fold) and spleen (30-fold), compared to the organ with lowest expression. NCCRP-1 gene expression was not induced by in vitro treatment of head kidney cells with polyinosinic polycytidylic acid (poly I:C) or lipopolysaccharide (LPS), or by in vivo injections with poly I:C or formalin killed Vibrio anguillarum. These results show that the cod NCCRP-1 gene is differentially expressed in organs, and that gene expression is not induced by the tested treatments.
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PMID:The gene structure and expression of the non-specific cytotoxic cell receptor protein (NCCRP-1) in Atlantic cod (Gadus morhua L.). 1736 63

Solea senegalensis is a commercially relevant aquaculture species that remains largely unexplored at the genomic level. The aim of this study was to identify novel genomic responses to lipopolysaccharide and copper sulphate challenges using suppression subtractive hybridization (SSH) and real-time RT-PCR. Forward- and reverse-subtractive libraries were generated for the identification of genes whose transcription is altered in response to lipopolysaccharide (LPS) (immunomodulator) in head kidney (immunologically important organ) and to CuSO(4) (common algacide) in liver (central metabolic organ and important source of immune transcripts). A total of 156 genes involved in major physiological functions were identified by SSH, the identified sequences representing a significant increase in the number of sole ESTs in public databases. Fifteen genes represented in the subtracted libraries were selected for further tissue, temporal and inducible transcriptional profiling by real-time RT-PCR. A rigorous quantification of transcript copy numbers was performed for this purpose in both pooled and individual samples from two independent experiments. More than half of the investigated mRNAs encode proteins that deal with different aspects of the immune response, like NCCRP1 (non-specific cytotoxic cell receptor), C3 and C7 (complement components), and ferritin M, HP and TF (iron homeostasis), or play a crucial role in its regulation, like TRAF3. Other mRNAs studied encode proteins involved in metabolism, like TKT and NDUFA4, the response to stimulus, like CEBPB (transcription factor) and CIRBP (RNA-binding protein), and other cell processes. Highly abundant (>500 molecules/pg total RNA) and rare (< or =1 molecules/pg) mRNA species were quantified in each sole organ examined, and outstanding differences were also recorded in the comparison between the two organs, e.g. C3 and TF mRNAs were largely overexpressed in liver (>5000 molecules/pg) as compared to head kidney (<5 molecules/pg). Most investigated mRNAs displayed significant alterations in their steady-state copy number following LPS and/or CuSO(4) stimulation, i.e. they were (i) up-regulated in response to both treatments in at least one of the two organs (NCCRP1, CEBPB, SQSTM1, NDUFA4, C7 and HP), (ii) up-regulated (TF, CIRBP, TRFA, C3) or down-regulated (TKT) by LPS, their levels remaining essentially unchanged upon CuSO(4) challenge, or (iii) down-regulated by LPS, though up-regulated by CuSO(4) (ferritin M). Quantifications in individual fish were consistent with those in pooled samples with respect to both the direction and the absolute changes in transcript abundance.
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PMID:Solea senegalensis genes responding to lipopolysaccharide and copper sulphate challenges: large-scale identification by suppression subtractive hybridization and absolute quantification of transcriptional profiles by real-time RT-PCR. 1907 Mar 73