Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P43026 (
lipopolysaccharide
)
62,215
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
It has been proposed that intracellular pathogens may interfere with expression or function of proteins that mediate vesicular traffic in order to survive inside cells. Brucella melitensis is an intracellular pathogen that evades phagosome-lysosome fusion, surviving in the so-called Brucella-containing vacuoles (BCV).
Vesicle-associated membrane protein 3
(
VAMP3
) is a v-SNARE protein that promotes the exocytosis of the proinflammatory cytokine TNF at the phagocytic cup when docking to its cognate t-SNARE proteins syntaxin-4 and SNAP-23 at the plasma membrane. We determined the expression level of
VAMP3
in J774.1 murine macrophages stimulated with B. melitensis
lipopolysaccharide
(
LPS
) and detected a transitory increase of
VAMP3
mRNA expression at 30 min. A similar result was obtained when cells were incubated in the presence of
LPS
from Salmonella enterica serovar Minnesota (SeM). This increase of
VAMP3
mRNA was also observed on infected cells with B. melitensis even after one hour. In contrast, infection with Salmonella enterica serovar Enteritidis (SeE) did not cause such increase, suggesting that membrane components other than
LPS
modulate
VAMP3
expression differently. To determine the effect of
VAMP3
inhibition on macrophages infection, the expression of
VAMP3
in J774.A1 cells was silenced and then infected with wild-type B. melitensis. Although a slight decrease in the rate of recovery of surviving bacteria was observed between 12 h and 36 h post-infection with B. melitensis, this was not significant indicating that
VAMP3
is not involved in Brucella survival.
...
PMID:Silencing of VAMP3 expression does not affect Brucella melitensis infection in mouse macrophages. 2307 44
