Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P43026 (
lipopolysaccharide
)
62,215
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Host defense peptides (HDPs) are multifunctional immune molecules that respond to bacterial and viral pathogens. In the present study, bone marrow-derived cells (BMCs) and chicken embryonic fibroblasts (CEFs) were cultured from a Leghorn line (Ghs6) and Fayoumi line (M15.2), which are inbred chicken lines relatively susceptible and resistant to various diseases, respectively. The cells were treated by
lipopolysaccharide
(
LPS
) or polyinosinic-polycytidylic acid (poly(I:C)) and, subsequently, mRNA expression of 20 chicken HDPs was analyzed before and after the stimulation. At homeostasis, many genes differed between the chicken lines, with the Fayoumi line having significantly higher expression (
p
< 0.05) than the Leghorn line:
AvBD1
,
2
,
3
, 4,
6
, and
7
in BMCs;
CATH1
,
CATH3
, and
GNLY
in CEFs; and
AvDB5
,
8
,
9
,
10
,
11
,
12
,
13
in both BMCs and CEFs. After
LPS
treatment, the expression of
AvBD1
,
2
,
3
,
4
,
5
,
9
,
12
,
CATH1
, and
CATHB1
was significantly upregulated in BMCs, but no genes changed expression in CEFs. After poly(I:C) treatment,
AvBD2
,
11
,
12
,
13
,
CATHB1
and
LEAP2
increased in both cell types;
CATH2
only increased in BMCs; and
AvBD3
,
6
,
9
,
14
,
CATH1
,
CATH3
, and
GNLY
only increased in CEFs. In addition,
AvBD7
,
AvBD14
,
CATH1
,
CATH2
,
GNLY
, and
LEAP2
showed line-specific expression dependent upon cell type (BMC and CEF) and stimulant (
LPS
and poly(I:C)). The characterization of mRNA expression patterns of chicken HDPs in the present study suggests that their functions may be associated with multiple types of disease resistance in chickens.
...
PMID:Induction of Chicken Host Defense Peptides within Disease-Resistant and -Susceptible Lines. 3306 61
