Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P43026 (lipopolysaccharide)
 62,215 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Two long-chain fatty acids, 27-oxo-octacosanoic acid (28:0(27-oxo)) and heptacosane-1,27-dioic acid (27:0-dioic) were identified for the first time in phenol-chloroform-petroleum ether extracts of Legionella pneumophila, indicating that they are constituents of lipopolysaccharide. The fatty acids were characterised by combined gas-liquid chromatography/mass spectrometry and proton nuclear magnetic resonance spectroscopy. Moreover, minor amounts of 29-oxo-triacontanoic (30:0(29-oxo)) acid and nonacosane-1,29-dioic acid (29:0-dioic) as well as 27-hydroxy-octacosanoic acid (28:0(27-OH)) were present in the phenol-chloroform-petroleum ether extract.
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PMID:Identification of 27-oxo-octacosanoic acid and heptacosane-1,27-dioic acid in Legionella pneumophila. 142 93

The chemical composition of lipopolysaccharides from Legionella erythra and Legionella oakridgensis was analysed. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed both lipopolysaccharides to have a smooth-type character. The polysaccharide part of both lipopolysaccharides contained D-mannose, D-glucose, D-glycero-D-mannoheptose, L-glycero-D-manno-heptose, 2-keto-3-deoxyoctonic acid, L-fucosamine, D-glucosamine, and glucosamine phosphate. In addition, L-rhamnose, glycerol phosphate, and glucose phosphate were identified in the polysaccharide part of L. erythra lipopolysaccharide. The main sugar identified in the lipid A part of both lipopolysaccharides, 2,3-diamino-2,3-dideoxy-D-glucose, was found to be substituted with a complex fatty acid composition including at least 16 different amide-linked 3-hydroxy fatty acids. Both lipopolysaccharides contained nonhydroxy fatty acids and the uncommon 27-oxo-octacosanoic acid, 29-oxotriacontanoic acid, and 27-hydroxyoctacosanoic acid. The lipopolysaccharide of L. oakridgensis also contained 29-hydroxytriacontanoic acid. The dioic long-chain acids heptacosane-1,27-dioic acid and nonacosane-1,29-dioic acid were only present in the lipopolysaccharide of L. erythra.
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PMID:Lipopolysaccharides of Legionella erythra and Legionella oakridgensis. 792 88

Main attention in the paper is paid to the study of lipid A, a component possessing endotoxic activity. Lipids A containing glucosamine disaccharide (representatives of Enterobacteriaceae family), and variants of lipid A differing from the toxic one either in the structure of carbohydrate core or in the spectrum of fatty acids are considered. They are either phototrophic, nodulating (Bradyrhyzobium species) or soil species (Nitrobacter and Thiobacillus) bacteria. Lipid A from lipopolysaccharides of over 25 species of bacteria (Rhodopseudomonas viridans, R. palustris, Pseudomonas diminuta, Phenylobacterium immobile, Brucella melitensis, B. abortus, Thiobacillus ferrooxidans, etc.) contains 2.3-diamino-2.3-dideoxyglucose (lipid ADAG); glucosaminouronic acid was found in Rhizobium trifolii and galacturonic acid in R. leguminosarum bvs. phaseoli, trifolii and viceae. Mixed lipids (lipid A and lipid ADAG) were found in Campylobacter jejuni. Considerable variations were registered in the nature of fatty acids. Thus, 27-oxy-octacosanoic acid (27-OH-28 : 0) was found in lipid A of the studied species of Rhizobiaceae except for Azorhizobium caulinodans. No correlations between the composition of the carbohydrate core and presence of this acid were established. Implementation of the synthesis of a complete as well as of partial lipid A structures has confirmed authenticity of the described structures. Five different epitopes identified by antibodies are present in the hydrophilic part of lipid A. The structure and biological role of the outer and inner cores are considered separately, main attention being paid to identification of the role of the KDO-containing zone. Since O-specific polysaccharide is the most known lipopolysaccharide component from the viewpoint of the structure and biological activity, this material is given in a general form.
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PMID:[The endotoxins of gram-negative bacteria: their structure and biological role]. 795 30

Four long-chain fatty acids, 2-hydroxy-27-oxo-octacosanoic acid (n28:0(2-OH,27-oxo)), 2-hydroxy-29-oxo-triacontanoic acid (n30:0(2-OH,29-oxo)), 2-hydroxy-heptacosane-1,27-dioic acid (27:0(2-OH)-dioic) and 2-hydroxy-nonacosane-1,29-dioic acid (29:0(2-OH)-dioic) were identified by GLC-MS analysis in the phenol-chloroform-petroleum ether (PCP) extracts of Legionella jordanis, L. maceachernii and L. micdadei indicating that they are constituents of lipopolysaccharide. Moreover, five long-chain fatty acids (28:0(27-OH), 28:0(27-oxo), 30:0(29-oxo), 27:0-dioic and 29:0-dioic) previously identified in L. pneumophila (Moll, H. et al., FEMS Microbiol. Lett., 97 (1992), 1-6) were also found in these species. This is to our knowledge the first report on the existence of long chain 2-hydroxylated (omega-1)-oxo fatty acids and 2-hydroxylated 1,omega-dioic fatty acids.
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PMID:Long-chain alpha-hydroxy-(omega-1)-oxo fatty acids and alpha-hydroxy-1,omega-dioic fatty acids are cell wall constituents of Legionella (L. jordanis, L. maceachernii and L. micdadei). 845 96