Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P43026 (lipopolysaccharide)
 62,215 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We have labeled exposed surface glycoproteins of mouse lymphoid cells by the galactose oxidase-tritated sodium borohydride technique. The labeled glyco-proteins were separated by polyacrylamide slab gel electrophoresis and visualized by autoradiography (fluorography). The major thymocyte surface proteins have molecular weights of 170,000 and 125,000. Thymocytes from TL antigen-positive mouse strains showed an additional band with a molecular weight of 27,000. Highly purified T lymphocytes contain two major surface glycoproteins with molecular weights of 180,000 and 125,000. Purified B lymphocytes have one major surface glycoprotein with a molecular weight of 210,000. When T lymphocytes are stimulated in vitro by concanavalin A or phytohemag-glutinin, the major proteins characteristic of T cells are relatively weakly labeled, but new components of lower molecular weights appear on the cell surface. A similar change is seen in B lymphocytes stimulated by Escherichia coli lipopolysaccharide. T lymphoblasts isolated from mixed lymphocyte cultures show a slightly different surface glycoprotein pattern. A polypeptide with a molecular weight of 57,000, which was labeled without enzymatic treatment by tritiated sodium borohydride alone, is strongly labeled in proliferating cells.
 ...
PMID:Characterization of surface glycoproteins of mouse lymphoid cells. 19 30

Comparative chemical analysis (methylation, gas chromatography/mass spectrometry, periodate oxidation, etc.) of the lipopolysaccharides and degraded polysaccharides derived from Shigella sonnei phase I, phase II and galactose-deficient R mutants revealed a structure as shown: (formula: see text) 3-Deoxy-D-manno-octulosonic acid (dOclA) as an immunodeterminant was observed in the passive hemolysis inhibition test by (a) selective inhibition of the phase II system by dOclA; (b) the kinetics of the change of serological activity during mild acid treatment: 1% acetic acid abolished serological activity; (c) a lack of activity in galactose-less R mutants and reactivity with Re mutants including Salmonella minnesota Re. An enhanced sensitivity of phase II lipopolysaccharide to galactose oxidase after prolonged treatment with 1% acetic acid suggests that dOclA is linked to C-6 of the terminal or subterminal galactose. dOclA as immunodeterminant could explain some different polysaccharide structures described for Escherichia coli R1 core.
 ...
PMID:Structure of the hexose region of Shigella sonnei phase II lipopolysaccharide with 3-deoxy-D-manno-octulosonic acid as possible immunodeterminant and its relation to Escherichia coli R1 core. 35 97

Neutral glycolipids, gangliosides, and phospholipids present on membranes of unstimulated or lipopolysaccharide (LPS)-stimulated B cells were analyzed in LPS-responsive C3H/HePAS and LPS-nonresponsive C3H/HeJ mice. In the set of neutral glycolipids, asialo GM1 reacted preferentially with galactose oxidase but was not detectable with monospecific antibodies during immunocytofluorescence analysis. Another, more polar, neutral glycolipid appeared exclusively after stimulation of responsive B cells. Among the membrane gangliosides 1 to 5 that were able to react with galactose oxidase on B cells, ganglioside 3 was not detected in the mutant strain, and its absence was counterbalanced by the presence of a larger amount of ganglioside 1. The biosynthesis of total membrane phospholipids and the balance between phosphatidylethanolamine and phosphatidylcholine were significantly different in the two mouse strains examined and were quantitatively and qualitatively modified during the mitogenic response to LPS.
 ...
PMID:Membrane glycolipid and phospholipid composition of lipopolysaccharide-responsive and -nonresponsive murine B lymphocytes. 348 29

Extraction of whole promastigotes of Leishmania tropica major and L. donovani with a mixture of hexane and isopropanol (3:2) yielded three fractions containing immunological activity: lipids, where the activity was determined by radioimmunoassay; a lipopolysaccharide-like (LPS-like), water-soluble precipitate, where activity was determined both by radioimmunoassay and double gel diffusion, and the phenol: water extract of the lipid-free promastigotes, where activity was followed by double gel diffusion. The use of a solid state, lipid-based radioimmunoassay for detection of leishmanial antigens provided a sensitive measure of their activity with a considerable degree of species and serotype specificity. We found antibodies to leishmanial lipids in sera from immunized rabbits, convalescent mice, and human patients with confirmed cases of cutaneous leishmaniasis or kala azar. There was very little activity in normal human or animal sera. Analysis by SDS-polyacrylamide gel electrophoresis of fractions from promastigotes surface-labeled with galactose oxidase and sodium borotritiate and preliminary immunochemical characterization of the LPS-like antigen showed that it contained galactose, but otherwise differed immunologically and chemically from excreted factor (EF), the best characterized leishmanial antigen.
 ...
PMID:Lipid and lipopolysaccharide-like antigens of Leishmania promastigotes. 400 12

Lymphocytes from 33 out of 63 patients with B-cell chronic lymphocytic leukaemia (B-CLL) were successfully stimulated for cytogenetic analysis by means of two B-cell mitogens: pokeweed mitogen and lipopolysaccharide-B, used after pretreatment of the cells with neuraminidase and galactose oxidase. All patients had abnormal clones in 30-100% of the cells analysed. Chromosomes more frequently involved were Nos. 1, 3, 6, 11, 12, 13 and 14. The most common abnormality was a marker 14q+ (breakpoint 14q32) seen in 17 cases; trisomy 12 was observed in seven cases. A clinical scoring system was used to investigate the correlation of chromosome abnormalities with prognosis. The group with 14q+ was often associated with features of progressive disease, namely; prolymphocytoid or Richter transformation, refractoriness to therapy, high WBC and advanced staging. A significant difference in survival was observed between patients with 14q+ and the rest: median survival from diagnosis being 45 months and over 64 months, respectively (P less than 0.05); when survival was calculated from the time of chromosome analysis the values were 8 months and more than 41 months, respectively (P less than 0.01). It is suggested that 14q+ is acquired during the evolution of CLL and that this development may be a key event in the clinical progression of B-CLL. Other abnormalities, including trisomy 12, were not found to be associated with a worse prognosis.
 ...
PMID:Prognostic significance of chromosome abnormalities in chronic lymphocytic leukaemia. 633 48

Treatment of intact cells of Salmonella typhimurium with galactose oxidase (EC 1.1.3.9) resulted in an extensive oxidation of the susceptible galactose residues in the lipopolysaccharides. Comparison with the extent of oxidation in isolated lipopolysaccharides indicated that most lipopolysaccharide molecules were located in the outer leaflet of the outer membrane in intact cells.
 ...
PMID:Asymmetric localization of lipopolysaccharides on the outer membrane of Salmonella typhimurium. 698 17