Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P43026 (lipopolysaccharide)
 62,215 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The present study was conducted to establish baseline profiles of various macrophage functions in four commercial broiler genetic lines designated as Lines 1, 2, 3, and 4. All experiments were carried out between 2 to 3 wk of age. Total numbers of peritoneal exudate cells per bird collected 42 h after a single i.p. injection of Sephadex-G50 were comparable among the lines. Line 1 produced fewer macrophages along with reduced phagocytosis of opsonized SRBC, whereas Line 4 macrophages were depressed in unopsonized SRBC phagocytosis. Macrophages from Lines 2 and 4 killed internalized Escherichia coli earlier than macrophages from Lines 1 and 3. Supernatants from lipopolysaccharide-treated macrophages from Lines 1 and 2 exhibited significantly higher cytolytic activity against LSCC-RP9 tumor cells when compared with supernatants from Line 3 and 4 macrophages. The current study demonstrates genetic variation among the four broiler lines for mononuclear phagocytic system functions.
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PMID:Comparison of macrophage function in several commercial broiler genetic lines. 195 55

The present study was conducted to determine the tumoricidal potential of chicken macrophages. Sephadex-elicited peritoneal macrophages from 6-wk-old Cornell K-strain White Leghorn females (B15B15) were used as effector cells against three different 51Cr-labeled tumor cell targets: LSCC-RP9 (B15B2), MDCC-CU14 (B19, C2), and MDCC-CU25 (B17B17). Quantification of tumoricidal activity was done in a 16-h, Cr-release assay. Macrophages collected at 12, 24, and 42 h post-Sephadex stimulation failed to kill any of the tumor cell targets. However, macrophages treated with concanavalin A stimulated splenic cell supernatants (lymphokines, LK) and lipopolysaccharide (LPS) were able to kill all three tumor cell targets in coculture experiments. Cell-free supernatants collected from LPS and LK alone or combination-treated macrophages demonstrated cytolytic activity for both RP9 and CU25 tumor cell targets. The results of the study, therefore, suggest that chicken macrophages acquire tumoricidal competence if treated with macrophage activation signals such as LK, or LPS or both.
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PMID:Signal requirements for the acquisition of tumoricidal competence by chicken peritoneal macrophages. 204 46

In an analysis of nitric oxide (.NO) production and toxicity, chicken macrophage-generated .NO inhibited mitochondrial activity in both .NO-producing macrophages themselves and lymphoid tumor targets. However, differences in targeting of mitochondrial toxicity were observed among these cells. Two chicken macrophage cell lines, HD11 and MQ-NCSU, produced .NO (measured as nitrite) dependent upon concentrations of L-arginine and bacterial endotoxin (lipopolysaccharide). Mitochondrial activity was negatively correlated with the amount of .NO produced. Using a modified MTT assay, .NO induced suppression in two mitochondrial complexes. Mitochondrial activity was significantly suppressed among HD11 cells receiving LPS alone (complex I, 63.0 +/- 5.5% suppression; complex II, 27.9 +/- 5.2%). In contrast, mitochondrial activities in samples receiving LPS plus inhibitor, NG-nitro-L-arginine methyl ester (NAME; 5 mM) or 2,4-diamino-6-hydroxypyrimidine (DAHP; 5 mM), were not significantly different from control values. When HD11 macrophages were cocultured with lymphoblastoid tumor targets, RECC-CU60 (T cell) or LSCC-RP9 (B cell), adding LPS (1 microgram/ml), tumor cell mitochondrial activity was significantly suppressed. In the generator macrophages, complex I was more suppressed than complex II, whereas in lymphoid targets no such difference was observed. These results indicate that .NO inhibits complex I and II mitochondrial activity but that differential targeting can occur among chicken leukocyte populations.
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PMID:Nitric oxide (.NO)-induced mitochondrial injury among chicken .NO-generating and target leukocytes. 802 70