Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P43026 (
lipopolysaccharide
)
62,215
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The aim of this study was to elucidate whether a novel mitochondrial antioxidant protein,
SP-22
, is localized in placenta and whether its expression is induced in placenta of
lipopolysaccharide
(
LPS
)-exposed mouse. Western blot analysis of normal human placenta indicated that the
SP-22
protein was located in the mitochondrial fraction. Immunohistochemical analysis of
SP-22
in normal placenta showed that immunoreactive
SP-22
was distributed mostly in cytotrophoblastic cells but with almost no signal in syncytiotrophoblasts. The positive signals were also detected in the decidual cells and stromal cells in stem villi of normal placenta. We also examined
LPS
-mediated inflammatory placenta on day 13 of pregnancy at various time points after
LPS
injection (50 microg/kg, intraperitoneally). Western blot analysis indicated that
LPS
approximately quadrupled the expression of
SP-22
in placenta of
LPS
-exposed mouse. When the
SP-22
protein was localized immunohistochemically, the decidua and the diploid trophoblasts in the basal zone were intensively stained in placenta of
LPS
-exposed mouse compared to the control. The localization and inducible expression of
SP-22
protein in placenta suggest a possible role in antioxidant system in mitochondria of normal and inflammatory placentae.
...
PMID:Expression of mitochondrial thioredoxin-dependent antioxidant protein, SP-22, in normal human and inflammatory mouse placentae. 1109 35
MER5
(also called peroxiredoxin III, PrxIII) is a member of peroxiredoxin family that has antioxidant activity. The present study was performed to investigate its in vivo function using
MER5
knockout mice.
MER5
knockout mice were born in normal frequency and could grow to maturity, but we found that intracellular ROS levels are significantly higher in the macrophages of the knockout mice. We examined roles of
MER5
function for the oxidative stress responses by intratracheal inoculation of
lipopolysaccharide
(
LPS
) to the mice. Lung inflammation such as inflammatory cell infiltration and airway wall thickening was more severely detected in the knockout mice. At the same time, oxidative damage on DNA and proteins was more strongly detected in lung tissues of the knockout mice, including 8-hydroxy-2'-deoxyguanosine (8-OHdG) formation and protein carbonylation. The degrees of lung inflammation and oxidative damage were positively related with
LPS
doses. Our results indicate that
MER5
knockout mice accumulated higher intracellular ROS levels, which cause
LPS
-induced lung injury more severely, and thus, suggested that
MER5
acts as an important scavenger of reactive oxygen species (ROS) under oxidative stress.
...
PMID:Increased susceptibility of MER5 (peroxiredoxin III) knockout mice to LPS-induced oxidative stress. 1731 58
