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Query: UNIPROT:P43026 (lipopolysaccharide)
 62,215 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The isolation and some properties of a lipopolysaccharide (LPS)-specific phage isolated against Pseudomonas aeruginosa is reported. The phage, designtaed omega PLS-I, is a Bradley A type phage with a head diameter of 70 nm and a contractile tail 120 nm long. The average adsorption rate constant for omegaPLS-I is 4-48 X 10(-9) ml/min. omegaPLS-I is inactivated by purified LPS from P. aeruginosa strain 1-1A, showing a PhI50 value of 1-25 mug/ml.
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PMID:The isolation and characterization of a lipopolysaccharide-specific Pseudomonas aeruginosa bacteriophage. 82 3

Phage H22 was isolated from sewage using Pseudomonas aeruginosa NCTC 8505 (serotype 0:3) as the host. Although not O-specific, this phage was found to have lipopolysaccharide (LPS) as a receptor. The broad host-range and lack of O-specificity of the phage suggested that its receptor site was in the core region of the LPS. Phage H22 had a Bradley type A structure. It was unaffected by chloroform and diethyl ether, and was stable between pH 5 and 8 and in the temperature range 0 to 60 degrees C. The adsorption rate constant was 14.6 X 10(-9) ml min-1. The phage had a latent period of 43 min, with a rise time of 18 min and a burst size of 6. The adsorption of phage to whole cells and LPS occurred over a broad pH range. Maximum adsorption occurred at 50 degrees C and pH 7.5 in the presence of 0.001 M Ca2+.
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PMID:Isolation and characterization of a lipopolysaccharide-specific bacteriophage of Pseudomonas aeruginosa. 308 73

A lipopolysaccharide (LPS)-defective (rough) mutant of Pseudomonas aeruginosa PAO was isolated by selection for resistance to the LPS-specific phage E79. The LPS of this mutant, AK-1012, lacked the O-antigenic side chain-specific amino sugar fucosamine as well as the core-specific sugars glucose and rhamnose. Using this strain, we isolated and characterized a phage, phi PLS27, which is specifically inactivated upon incubation with LPS extracted from rough mutants of P. aeruginosa PAO. phi PLS27 was found to be a Bradley type C phage and was very similar to coliphage T7 in a number of properties, including size, buoyant density, mass, and the number of structural proteins.
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PMID:Isolation and characterization of a bacteriophage specific for the lipopolysaccharide of rough derivatives of Pseudomonas aeruginosa strain PAO. 678 14

Four bacteriophages, P22, P27, 9NA, and KB1, active on smooth Salmonella strains belonging to serogroups A, B, and D1 were investigated for endoglycosidase activity and specificity in enzyme hydrolysis assays. Purified phage was incubated with phenol-water-extracted lipopolysaccharide preparations which had been partially delipidated. Dialyzable oligosaccharides, released by phage glycosidase activity, were analyzed by sugar and methylation analyses. Phages P27, 9NA, and KB1, as well as P22 assayed earlier (U. Eriksson et al., J. Gen. Virol. 43: 503-511, 1979; S. Iwashita and S. Kanegasaki, Biochem. Biophys. Res. Commun. 55:403-409, 1973), were all found to have phage-associated endorhamnosidase activity hydrolyzing the O-polysaccharide chain common to bacteria of serogroups A, B, and D1 [Formula: see text] between the l-rhamnose and d-galactose residues. The nature of the R monosaccharide, abequose, tyvelose, or paratose, had no effect on the activity or specificity of the endorhamnosidase, whereas a change of the d-galactose --> d-mannose linkage from alpha1,2 to alpha1,6 made the O-polysaccharide chain resistant to the endorhamnosidases. Modification of the O chain by glucosylation of the d-galactose residue at O-4 or O-6 revealed two glycosidase specificities: the phage P22 and P27 enzymes hydrolyzed O chains glucosylated at O-4 but not O-6, whereas the phage 9NA and KB1 enzymes hydrolyzed chains glucosylated at O-6 but not O-4. Phage KB1, like P22 and P27, had a short, noncontractile tail containing a base plate with tail spikes (morphologically Bradley group C), whereas 9NA had a long, flexible tail ending with a base plate-like appendage (Bradley group B), which suggests that the endorhamnosidase activity can be associated with different tail structures.
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PMID:Salmonella bacteriophage glycanases: endorhamnosidase activity of bacteriophages P27, 9NA, and KB1. 701 63

Four bacteriophages recognizing the Escherichia coli lipopolysaccharide (LPS) antigens O4, O5, O6, and O7, respectively, were isolated from pooled sewage samples. Electron microscopic investigations revealed icosahedral phage structures. Phages phi O4, phi O5, and phi O7 belonged to Bradley's morphology group C, while phi O6 had a tail and resembles phages of group A of Bradley. The nucleic acid of the phages was identified as double-stranded DNA of different genomic sizes. Host range studies showed that only E. coli strains with homologous O antigens were attacked. No lysis of encapsulated and rough E. coli strains was observed. The phages specifically depolymerized the homologous LPS of their host strains; they may be useful for detecting respective non-capsulated E. coli strains in epidemiological studies as simple alternative to the laborious serological typing. Diagnostic application is restricted, however, as strains carrying K antigens have not been detected. The high specificity of the phage-associated enzymes provides a mild method for the preparation of oligosaccharides from the LPS for structural studies.
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PMID:Bacteriophages specifically recognizing the lipopolysaccharide antigens O4, O5, O6, and O7 of Escherichia coli. 753 32