Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P43026 (lipopolysaccharide)
 62,215 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Transductional mapping, with phage ES18 or ES18.hl, showed that several mutations causing the RfaH- phenotype (defective formation of galactose I and also of more distal units of the lipopolysaccharide core) were located between metE and pepQ in the Salmonella typhimurium linkage map; the affected locus is designated rfaH. The mutation of one strain of RfaH- phenotype was located elsewhere, at an unidentified rfa locus. Introduction of an F' plasmid containing the metE segment of the Escherichia coli chromosome into several rfaH mutants restored the "smooth" (Rfa+) phenotype. Several rfaH mutations, and that of the phenotypically similar rfa mutant, caused increased sensitivity to bacitracin, polymyxin, novobiocin, nafcillin and oxacillin, as expected if the mutations have no effect on the formation of the part of the lipopolysaccharide core proximal to the galactose units.
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PMID:Salmonella typhimurium mutants of RfaH-phenotype: genetics and antibiotic sensitivities. 698 42

Loci termed rfa, determining biosynthesis of somatic lipopolysaccharide core, have been mapped in Salmonella typhimurium LT2. The smooth-specific phage P22 co-transduced two leaky rfa alleles with cysE and with pyrE; one of the leaky alleles is perhaps rfaG, and the other is an unidentified gene concerned with synthesis of the heptose-containing part of the core. The lipopolysaccharide-indifferent phage ES18 (or its variant ES18.h1) co-transduced rfaF, rfaG, rfaL, rfa(R-res-1), and rfa(R-res-2) alleles with cysE and with pyrE, at rates indicating the order cysE-rfaF-(rfa[R-res-1], rfa[r-res-2], rfaL)-rfaG-pyrE. One proven (and two suspected) rfaE alleles and five proven rfaH alleles were not co-transduced with cysE or pyrE. Hfr crosses indicated that the proven rfaE allele mapped between serA and strA.
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PMID:Mapping of rfa Genes in Salmonella typhimurium by ES18 and P22 Transduction and by Conjugation. 1655 60