Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P43026 (lipopolysaccharide)
 62,215 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Hepcidin is a cysteine-rich, dual-function peptide with antimicrobial activity that plays a crucial role in iron homeostasis. Here, we have identified two hepcidin-like cDNA sequences from pigeon, Columba livia. The two cDNAs consist of 295 and 380 nucleotides, respectively, and were named HP1 and HP2. Sequence alignment showed that the homology between pigeon and mammals or amphibians is higher than that of pigeon and fishes. Semi-quantitative RT-PCR analysis suggested that HP1 transcripts are highly abundant in liver, abundant in spleen, less abundant in kidney and muscle, and undetectable in brain and intestine. However, HP2 are strongly expressed in the liver, spleen, kidney and muscle, weakly in the intestine, and not in the brain. After pigeon were submitted either to lipopolysaccharide (LPS) infection or iron-dextran stimulation, the hepcidin transcript levels were analyzed by a comparative RT-PCR. The results revealed that the expression of hepatic HP1 dramatically increased at 6 h post-infection of LPS injection, then gradually declined to normal levels. HP1 mRNA in the liver was 4.5-5-fold increase compared with the control animals after one week in iron-dextran injection pigeons. Interestingly, liver HP2 expression was only significant increase in the LPS infection pigeons, and not statistical change in iron-dextran stimulation ones. All these results indicate that the two hepcidins-like may have different functions in pigeon.
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PMID:Identification and expression analysis of hepcidin-like cDNAs from pigeon (Columba livia). 1761 50

In this work, we developed a sensitive and efficient ratiometric electrochemical method for lipopolysaccharide (LPS) detection using Cu-based metal-organic frameworks (Cu-MOFs) as a catalyst and target-triggered quadratic cycles for signal amplification. First, in the presence of target LPS, the conformation change of the specifically designed hairpin probes 1 (HP1) triggered the target cyclic-induced polymerization to produce the output DNA with the aid of phi29 DNA polymerase (phi29). Then, the obtained output DNA hybridized with ferrocene-labeled hairpin probes 2 (Fc-HP2, which were immobilized on the electrode) to generate a nicking endonuclease (N.BstNBI) cleavage site. Thus, with N.BstNBI, the original signal molecules of Fc left from the electrode, and the single-stranded capture-probe-modified sensing interface was obtained. At this time, signal probes conducted by Au-nanoparticles-functionalized Cu-MOFs and labeled hairpin probes 3 (HP3/AuNPs/Cu-MOFs) were hybridized with capture probes for hairpin assembly. Herein, AuNPs/Cu-MOFs were not only used as nanocarriers for immobilizing HP3 but also acted as electroactive materials for signal reporting. With the proposed target-triggered quadratic cycles, the cleavage sites of Fc-HP2 were cut, and capture probes were obtained to hybridize with HP3/AuNPs/Cu-MOFs, which caused the signal decrease of Fc. Then Cu-MOFs were closed to the electrode for the signal increase of Cu-MOFs. Furthermore, when glucose was present in the detection solution, AuNPs/Cu-MOFs catalyzed the oxidation of glucose to realize the enzyme-free signal amplification. By measuring the peak currents ratio of the Cu-MOFs and Fc, the proposed aptasenor for LPS detection showed a low detection limit (0.33 fg/mL) and a wide linear range from 1.0 fg/mL to 100 ng/mL with high accuracy and sensitivity. This ratiometric electrochemical approach is expected to be a valuable strategy for detection of other analytes.
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PMID:Cu-Based Metal-Organic Frameworks as a Catalyst To Construct a Ratiometric Electrochemical Aptasensor for Sensitive Lipopolysaccharide Detection. 2646 56