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Query: UNIPROT:P43026 (lipopolysaccharide)
 62,215 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The recruitment of polymorphonuclear leucocytes (PMNs) during the development of experimental pyogranulomas induced by Corynebacterium pseudotuberculosis was followed in nine male lambs by scintigraphic examination. Autologous blood PMNs were labelled with 99m-technetium or 111-indium and were re-injected intravenously into infected lambs. The functional properties of the labelled cells were monitored 1) in vitro by measuring their phagocytic and bactericidal activity against C. pseudotuberculosis and their chemotaxis under agarose, and 2) in vivo by following scintigraphically their capacity to accumulate in an inflammatory focus induced by intradermal injection of latex beads coated with Salmonella abortus equi lipopolysaccharide. Following inoculation of corynebacteria into the right ear of lambs, radioactive foci were observed to be localized in the right ear and in the draining lymph nodes during the 4 days following inoculation. Histopathological examination performed 32 h after inoculation confirmed the intense accumulation of PMNs at these sites. With the exception of one animal, which presented visible foci in the neck 14 days postinoculation, no radioactive foci were observed during the later phases of experimental infection, despite the presence of multiple pyogranulomas which were confirmed by bacteriological examination after necropsy of the lambs. Histopathological examination of these lesions revealed layers of fibroblasts, lymphocytes, and macrophages surrounding a necrotic centre. The results of these studies suggest that the contribution of PMNs during the chronic phase of inflammation is considerably reduced in comparison with the acute inflammatory phase of the infectious process.
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PMID:Recruitment of 99m-technetium- or 111-indium-labelled polymorphonuclear leucocytes in experimentally induced pyogranulomas in lambs. 216 66

Two forms of lipopolysaccharide-protein complex with buoyant densities of 1,43 and 1,40 g/cm3 were found in the Yersinia pseudotuberculosis cell wall. These forms have the similar monosaccharide, fatty acid and polypeptide compositions, but differ in the length of O-specific chains. The differences in density are stipulated by the different contents of the main components of the complex. Both forms contain the related antigenic determinants but have some differences in the antigenic structure. The ability of the two forms to produce a hybrid form with the intermediate density of 1,41 g/cm3 has been shown.
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PMID:[Different forms of a lipopolysaccharide-protein complex from Yersinia pseudotuberculosis]. 241 36

A branched chain octose, 3,6-dideoxy-4-C-(L-glycero-1'-hydroxyethyl)-D-xylo- hexose, was isolated from the lipopolysaccharide of Yersinia frederiksenii, serovar O: 16.29 and identified as yersiniose A from Y. pseudotuberculosis, serovar VI. Mild hydrolysis of the lipopolysaccharide with acetic acid afforded a rhamnan. Structural features of the trisaccharide repeating unit were elucidated on the basic of 13C NMR spectral data, methylation studies and periodate oxidation. Using these data as well as data on sugar composition and methylation studies of the lipopolysaccharide, the following structural pattern of the repeating unit of O-specific polysaccharide was proposed: (formula; see text)
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PMID:[The structure of O-specific polysaccharide of Yersinia frederiksenii serotype O:16,29 lipopolysaccharide]. 248 41

Twenty two hybridoma strains producing monoclonal antibodies against Francisella tularensis ATCC 6223, var. tularensis, were characterized. In an enzyme-linked-immunosorbent-assay (ELISA) using formaldehyde fixed bacteria as antigens, neither cross-reactions with six different Brucella spp., with Yersinia enterocolitica 0:9 nor with two biotypes of Yersinia pseudotuberculosis could be detected. The antibodies gave comparable titres with the three strains of F.tularensis tested. ELISA binding studies indicated that fifteen of the antibodies bound with high affinities to their epitopes of the three Francisella strains, while the others each seemed to bind with low affinity to at least one of the antigens. Immunoblot analysis showed that six of the antibodies were directed to epitopes on the core moiety of the lipopolysaccharide molecule, while the other 16 antibodies bound to O side chain components.
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PMID:Monoclonal antibodies reacting specifically with Francisella sp. 259

The pore-forming protein, lipopolysaccharide-protein complex and lipopolysaccharide of Y. pseudotuberculosis outer membrane have been shown to participate in the penetration of the bacteria into the cells of the macroorganism, to produce a toxic effect on these cells and to enhance the ingesting activity of macrophages in small doses, while suppressing it in large doses. When introduced parenterally, protein induces a more pronounced clinical picture of specific reactive hepatitis in experimental animals and greater changes in their kidneys than the lipopolysaccharide--protein complex.
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PMID:[Components of the outer membrane of Yersinia pseudotuberculosis and their role in the pathogenesis of pseudotuberculosis]. 301 47

Enteropathogenic Yersinia sp. releases plasmid-associated proteins of low molecular mass (26-67 kilodaltons) at 37 degrees C. In this study, the optimum conditions for the release of proteins were assessed and the released proteins (RPs) were analyzed for the manner of release, immunochemical characteristics, and the location of the genes necessary for their synthesis. Protein release was strongly enhanced when growth media were markedly depleted of calcium ions by precipitation with oxalate or chelation with EGTA [ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid]. RP yields were greatest when Yersinia spp. were in the exponential growth phase. The RPs appeared to be released from the Yersinia spp. by secretion rather than by pinching off of membrane vesicles, because the RPs did not sediment during high-speed centrifugation nor were they contaminated to any significant degree with lipopolysaccharide. Moreover, immunoblot analysis revealed only traces of protein species related to RPs within the outer membranes of plasmid-positive Yersinia spp. grown at 37 degrees C under calcium-restricted conditions. Immunoblot studies also showed that the RPs of Y. enterocolitica serotypes O:3, O:8, and O:9 and the RP of Y. pseudotuberculosis serotype I are highly cross-reactive. Finally, the immunoprecipitates of the products of minicells which harbor Yersinia plasmids were used to demonstrate that at least three proteins immunochemically related to the released fraction were plasmid encoded. These results suggest that at least three of the RPs may be related to or identical with previously described plasmid-encoded Yersinia outer membrane proteins.
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PMID:Immunochemical analysis of plasmid-encoded proteins released by enteropathogenic Yersinia sp. grown in calcium-deficient media. 377 Sep 52

Study of the effect of lipopolysaccharide (LPS) of Y. pseudotuberculosis on the culture of peritoneal macrophages of guinea pigs has established that in concentrations of 1-10 micrograms/ml LPS stimulated the absorption and digestive activity of the macrophages. In concentrations of 50-100 micrograms/ml it had a cytotoxic effect on the cell elements and inhibited their phagocytic function. The study of the LPS effect on absorption and liberation of 14C-labeled bacteria of Y. pseudotuberculosis showed that the biopolymer increased 2-3 times the uptake of the labeled antigen from the macrophages and lowered its elimination. In the experiments on mice infected with a labeled culture of S. aureus no increase in the uptake under the effect of the pseudotuberculosis LPS was observed, whereas an increase in the rate of the label elimination was stated. The difference was statistically significant. The results of the experiments are indicative of the specific effect of LPS. The study demonstrated a significant role of LPS of Y. pseudotuberculosis in the pathogenesis and protection of the host from this infection.
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PMID:[Effect of lipopolysaccharide of the pseudotuberculosis bacterium on the functional activity of macrophages]. 389 Jul 20

Lipopolysaccharide prepared from cells of Yersinia (Pasteurella) pseudotuberculosis of serogroups I, II, III, IV, and V is known to contain the 3,6-dideoxyhexose (DDH) paratose, abequose, paratose, tyvelose, and ascarylose in its respective O-specific side chains. Lipopolysaccharides or lipid-free polysaccharides of all of the 10 known serogroups and subgroups were subjected to methylation analysis and determined as alditol acetates by gas-liquid chromatography and mass spectrometry. The results indicated that the O-specific side chains of nine serotypes are composed of oligosaccharide repeating units in the form of four alternative general structures in which a terminal DDH may vary. These structures are DDH [Formula: see text] 6-deoxy-d-manno-heptose [Formula: see text] d-galactose (serogroups IA, IIA, and IVB), DDH [Formula: see text] d-mannose [Formula: see text] l-fucose (serogroups IB and IIB), and two configurations similar to the latter except that the 4-position of l-fucose was either linked to the d-mannose residue (serogroups VA and VB) or to the DDH residue (serogroups III and IVA). In contrast, O-groups in lipopolysaccharide of the newly discovered serogroup VI contained the DDH colitose and 2-acetamido-2-deoxy-d-galactose. Accordingly, all five known types of DDH have now been detected in lipopolysaccharides of Y. pseudotuberculosis. The sugar 6-deoxy-d-manno-heptose, present in O-specific side chains of serogroups IA, IIA, and IVB, has not yet been reported to occur elsewhere in nature.
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PMID:Structure of O-specific side chains of lipopolysaccharides from Yersinia pseudotuberculosis. 481 91

An O-specific polysaccharide from the lipopolysaccharide Yersinia pseudotuberculosis 1A serovar has been isolated and characterized. This compound was shown to contain residues of paratose, 6-deoxy-D-manno-heptose, D-galactose and 2-amino-2-deoxy-D-glucose in equimolar ratios. Using methylation studies, partial acid hydrolysis and 13C NMR spectroscopy, the following structure was proposed for the repeating unit of the O-specific polysaccharide: (Formula: see text).
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PMID:[Structure of O-specific polysaccharide isolated from the Yersinia pseudotuberculosis serotype 1A lipopolysaccharide]. 620 36

The comparative studied on lipopolysaccharides from Yersinia pseudotuberculosis IVA serovar, strains 32 and 31D, have been conducted. The identity of the lipopolysaccharides isolated from these strains has been shown. The structural pattern of the repeating unit of the O-specific side chain of the lipopolysaccharide has been suggested: (Formula: see text)
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PMID:[Study of a lipopolysaccharide from Yersinia pseudotuberculosis of the IVA serotype]. 620 39


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