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Query: UNIPROT:P43026 (lipopolysaccharide)
 62,215 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Lipid A isolated from lipopolysaccharide of Yersinia pseudotuberculosis was used for immunization of rabbits to afford antisera to lipid A with titers of 1:640 in the passive hemolysis test. Exhaustion of immune serume with sheep erythrocytes decreased antibody titers up to 1:160. Authentic samples of 2-(DL-3-hydroxytetradecanoyl)amino-2-deoxy-D-glucose 6-phosphate, 2-tetradecanoylamino-2-deoxy-D-glucose 6-phosphate and 2-acetamido-2-deoxy-D-glucose 6-phosphate have been synthesized in order to carry out a comparative study of inhibitory activity of these compounds and lipid A using a system of lipid A and antiserum to lipid A. As a result, the immunodominant moiety of the lipid A of Y. pseudotuberculosis proved to contain a D-glucosamine residue acylated with 3-hydroxytetradecanoic acid at the amino group. The nature of the fatty acid acylating the amino group of glucosamine does not play an important role in the structure of immunodominant moiety of lipid A.
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PMID:Structural studies on the immunodominant group of lipid A from lipopolysaccharide of Yersinia pseudotuberculosis. 8 32

A comparative study of various procedures of a lipopolysaccharide-protein complex (LPPC) from Yersinia pseudotuberculosis was carried out. The materials obtained were fractionated by molecular-sieve chromatography on Sepharose 2B resulting in highly aggregated complexes with antigen activity. LPPC aggregates dissociated in the presence of sodium dodecylsulphate (SDS) and urea. The chemical composition and serologic properties of fractions obtained are under consideration. The protein component of the complex consists of two major polypeptides (molecular weights--45,000 and 20,000) and some minor ones. The LPS component appeared to give 2--3 narrow bands in gel under conditions of SDS-polyacrylamide gel electrophoresis. It is suggested that such fractionation is caused by LPS association-dissociation in the course of electrophoresis.
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PMID:Studies on a lipopolysaccharide-protein complex from Yersinia pseudotuberculosis. 1 isolation and characterization. 54

Lipid A was isolated from lipopolysaccharide of Yersinia pseudotuberculosis S form (strain 341, subtype IB) using mild hydrolysis with acetic acid. The purified material (yield about 25%, molecular weight about 2900) contained D-glucosamine (11%), fatty acids (54%), protein concomitant (9.7%) and phosphorus (approximately 2%). Dodecanoic and 3-hydroxy-tetradecanoic acids in a molar ratio of 1 : 3.6 were detected as major fatty acid constituents. The hydroxyl groups of D-glucosamine were acylated with the residues of both fatty acids, while the amino groups were substituted with the residue of 3-hydroxy-tetradecanoic acid. Such a simple fatty acid composition is reminiscent of that found in lipid A in Y. pestis.
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PMID:Studies on lipid A from Yersinia pseudotuberculosis lipopolysaccharide. Isolation and general characterization. 69 14

It was shown that Y. pseudotuberculosis strains causing the Far-Eastern scarlatina-like fever in the Primorsk region belonged to subtype IB-ipopolysaccharides of the standard strain of subtype IB and of the local strain were closely affiliated by the analytic data and monosaccharide composition, but differed from the lipopolysaccharide of strains belonging to subtype IA. Living vaccines should be used to obtain the sera against the subtypes IA and IB of the causative agent.
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PMID:[A comparative immunochemical study of the subtypes of serologic type I of the agent of pseudotuberculosis]. 109 7

The role of phagocytosis in experimental pseudotuberculosis was demonstrated in vivo and in vitro. Virulent Y. pseudotuberculosis strain caused the death of the majority of the cells, whereas the weakly-virulent ones--degenerative changes and the death of but of few of them. The capacity of the causative agents of pseudotuberculosis to survive and to reproduce within the cells could be regarded as one of the significant factors of virulence of these microbes. The maximal immunizing effect was observed in infection of the animals with sublethal dose of the living Y. pseudotuberculosis culture. A somewhat lesser, but a sufficiently high, immunizing effect was produced by lipopolysaccharide. A method of macrophage culture can be used for the assessment of the immunogenicity of various antigenic complexes of the microbes.
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PMID:[Interaction of the causative agent of pseudotuberculosis with the peritoneal macrophages from an immune and a nonimmune organism]. 110 25

Soluble mediators such as tumor necrosis factor alpha (TNF-alpha) may be important in the pathogenesis of many chronic pulmonary infections. We examined the ability of Corynebacterium pseudotuberculosis, Pasteurella haemolytica, and ovine lentiviruses (OvLV) to induce TNF-alpha secretion by pulmonary alveolar macrophages (PAM). Bronchoalveolar lavage cells, composed of greater than 90% PAM, were obtained from normal sheep. Bronchoalveolar lavage cells were cultured for 2, 24, 48, 72, or 168 h in endotoxin-free RPMI medium (with 10% autologous serum) or in medium containing one of the following additives: lipopolysaccharide, 1-micron polystyrene beads, C. pseudotuberculosis, P. haemolytica, or one of two plaque-cloned OvLV, 85/28 or 85/34. Lipopolysaccharide, C. pseudotuberculosis, and P. haemolytica induced TNF-alpha activity in PAM cultures as early as 2 h after inoculation, as assessed by a colorimetric cytotoxicity assay. This activity could be blocked by rabbit anti-recombinant bovine TNF-alpha serum. In contrast, medium alone, polystyrene beads, and productive infection by OvLV did not induce TNF-alpha activity in PAM cultures. Bacterial pathogens which infect pulmonary macrophages may elicit the secretion of TNF-alpha within the lungs and lead to the cachectic state associated with chronic pneumonia.
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PMID:Differential induction of tumor necrosis factor alpha in ovine pulmonary alveolar macrophages following infection with Corynebacterium pseudotuberculosis, Pasteurella haemolytica, or lentiviruses. 165 61

The capacity of Y. pseudotuberculosis strains for disassociation with the appearance of S- and P-forms has been studied. Strains 852 and 9547 show high stability in S-forms, their conversion into R-forms occurring at 40-42 degrees C. Strain 6953 shows pronounced polymorphism and instability of its associations at different growth temperatures. Strain 9532 exists in S- and R-forms which retain their stability during numerous subculturings at different growth temperatures and prolonged storage. This strain has plasmids of 130, 72.2, 5.7 kb. All plasmids are retained in S- and R-forms, i. e. the dissociation of the strain is not accompanied by the loss of plasmids. The conversion of the strain from the S-form into the R-form leads to changes in the structure of lipopolysaccharide and the composition of low-molecular (less than 23 kD) proteins in the outer and inner membranes. In tests on guinea pigs the LD50 of the R-form of the strain is tenfold greater than that of its S-form. The dissociants of strain 9532 are transformed by plasmid DNA with equal efficiency and equally inherit them without selective pressure.
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PMID:[The heterogeneity of collection strains of Yersinia pseudotuberculosis and their molecular biological properties]. 185 69

An O-specific polysaccharide has been isolated on mild acid hydrolysis of lipopolysaccharide from Yersinia pseudotuberculosis serovar IIc and shown to consist of abequose, D-mannose and 2-acetamido-2-deoxy-D-galactose residues in the ratio 0.8:3:1. From the results of acid hydrolysis, 13C NMR, methylation and periodate oxidation studies the structure of the repeating unit of the O-specific polysaccharide is deduced as follows: (formula; see text)
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PMID:[Structure of the O-specific polysaccharide chain of the Yersinia pseudotuberculosis lipopolysaccharide (serovar II C)]. 186 85

Experimental studies on guinea pigs have shown that Y. pseudotuberculosis lipopolysaccharide is capable of inducing endotoxinemia accompanied by the development of the thrombohemorrhagic syndrome. In cases of pseudotuberculosis the importance of the increased synthesis of prostaglandins and cyclic nucleotides with the prevalence of PGF2 alpha and cGMP in the genesis of toxico-allergic manifestations of pathologic processes has been established. The pathomorphological picture of pseudotuberculosis endotoxinemia is characterized by sludge, the vascular thrombosis of the microcirculatory bed, diapedetic hemorrhages, delymphatization of the immunogenetic organs. The moderately pronounced action of indomethacin, a prostaglandin inhibitor, on the manifestations of pseudotuberculosis endotoxinemia has been revealed.
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PMID:[The biological action of Yersinia pseudotuberculosis endotoxin]. 196 23

Eleven species are actually recognized within the genus Yersinia of which three--Y. pestis, Y. pseudotuberculosis, and certain serovars of Y. enterocolitica--are important infectious organisms for humans and warm-blooded animals. The causative agents of yersiniosis, Y. pseudotuberculosis and Y. enterocolitica, occur world-wide in areas of moderate and subtropical climate. Asymptomatically infected warm-blooded animals, causing environmental contamination, are the most important factors for the epidemiology of yersiniosis; apathogenic Yersinia species and serovars, on the other hand, are largely adapted to environmental conditions and, with regard to their ecology, independent from warm- or cold-blooded host organisms. The agents are usually transmitted by the oral route with food-stuffs as the most important vehicle of human yersiniosis. For their isolation, enrichment procedures and selective media have been developed. The organisms are identified by biochemical reactions and can be differentiated by serological and, in case of Y. enterocolitica, also by phage-typing methods. The differentiation of pathogenic and apathogenic strains is of diagnostic importance and should be routinely performed; simple tests are available for this purpose. Demonstration of antibodies against cell-wall-associated (lipopolysaccharide) or plasmid-encoded (protein) antigens may confirm the diagnosis if the causative agents failed to be isolated.
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PMID:[Microbiology and epidemiology of Yersinia infections]. 207


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