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Query: UNIPROT:P43026 (lipopolysaccharide)
 62,215 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The polysaccharide of the O-specific side chain of the lipopolysaccharide from the Yersinia pseudotuberculosis VA serovar has been isolated and studied. The structural pattern of the chemical repeating unit of the specific polysaccharide has been proposed as follows: (formula; see text).
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PMID:Structural studies on the O-specific side-chain polysaccharide of lipopolysaccharide from the Yersinia pseudotuberculosis VA serovar. 683 55

Yersinia enterocolitica S and R strains change the pattern and the amount of their lipopolysaccharide-linked fatty acids with the respective growth temperature. As described also for other enterobacterial strains, saturated fatty acids are decreased, especially the amount of C14:0, when growth was done at 10 degrees C and unsaturated fatty acids, notably C16:1 (palmitoleic acid), appear, which are present in trace amounts only when bacteria are grown at 40 degrees C. In addition to these changes in the fatty acids, also the amount of O-specific sugars is temperature-dependent. 6-Deoxy-L-altrose which is the only main O-specific sugar in the LPS investigated, amounts to 30-40% (based on LPS dry weight) when grown at 10 degrees C, but only to 13-20% when growth was done at 40 degrees C. The decrease in O-specific material at 40 degrees C can at least partly be explained by the finding of a high number of unsubstituted R core stubs in polyacryl gel-electrophoresis.
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PMID:Temperature-dependent changes in the sugar and fatty acid composition of lipopolysaccharides from Yersinia enterocolitica strains. 685 45

Yersinia enterocolitica (Y. ent.) infections are rather frequently complicated by acute reactive inflammation in the connective tissue, especially in the joints. At this stage of the disease the specific diagnosis can be obtained either by bacterial isolation and identification from the feces and/or mesenterial lymph nodes, or by serological methods. Serodiagnostics are frequently the only method during the complication phase, since the bacteria have often disappeared from the feces by this stage of the disease. Specific Y. ent. serodiagnostics are benefitted by the fact that no antisera cross-react with the serotype 3 thermostable O-antigen. A titre of greater than or equal to 80 is therefore highly indicative of a recent or current Y. ent. infection. In the absence of other known arthritogenic agents the Y. ent. antibodies are highly indicative of the Y. ent. etiology of a current disease. The Y. ent. complications affect most inflammatory reactive diseases, acute as well as chronic. In an area in which Y. ent. infections are endemic, Y. ent. is the most frequent cause of acute and chronic arthritis. The present results indicate that not all cases of acute Y. ent. arthritis remit, but some persist, usually with an intermittent course, and develop into rheumatoid arthritis or allied conditions. This suggests a common pathogenic mechanism in most inflammatory rheumatic diseases. It is proposed that the time has come for a classification of these diseases based on their etiology, in order to replace the present symptom-based treatment with a causal one, and to institute prophylactic measures. The pathology is not exclusive to Y. ent., but can presumably also be brought about by other bacteria, such as gonococci, meningococci, salmonellae, shigellae, and brucellae, possibly by their content of lipopolysaccharide.
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PMID:Yersinia enterocolitica infections and rheumatic diseases. 696 31

Rabbits were immunized with the enterobacterial common antigen (ECA)-immunogenic strain Escherichia coli F470. ECA-specific antiserum was obtained by absorbing the resulting antisera with the genetically closely related ECA-negative strain E. coli F1283. These two strains also served as positive and negative controls in the localization study of ECA in Yersinia enterocolitica strain 75, smooth and rough forms (Ye75S and Ye75R), by the indirect immunoferritin technique. Cells of Ye75S grown at 22 degrees C showed no labeling with ferritin after treatment with the ECA-specific antiserum and subsequent ferritin-conjugated goat anti-rabbit antibodies. If the cells were grown at 40 degrees C, however, most of the cells showed weak ferritin labeling. At this higher growth temperature, the lipopolysaccharide of this strain contains less O-specific chains (6-deoxy-L-altrose), as was shown in a previous study. The rough mutant Ye75R, which lacks O-specific chains completely, showed denser labeling with ferritin. These results indicate that ECA on the cell surface of Ye75S is covered by O-specific chains of the lipopolysaccharide if grown at 22 degrees C and is therefore not accessible to ECA antibodies. It becomes accessible, however, when O-chains are lacking (R mutants) or when they are reduced in size or amount (growth at 40 degrees C).
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PMID:Localization of enterobacterial common antigen in Yersinia enterocolitica by the immunoferritin technique. 702 35

The use of 13C-NMR spectroscopy in the establishment of lipid A backbone structure from lipopolysaccharide of Yersinia pseudotuberculosis has been described. The 13C-NMR spectra of degraded lipid A and its N-acetate were obtained. The assignment of signals was made by comparison with the chemical shifts for 13C-NMR spectra of glucosaminitol, N-acetyl-glucosaminitol and their beta-1,4 and beta-1,6 disaccharides. It was shown that lipid A backbone of the lipopolysaccharide in question consists of beta-1,6-linked glucosamine disaccharide.
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PMID:The application of 13C-NMR spectroscopy to study lipid A from Yersinia pseudotuberculosis lipopolysaccharide. 712 94

Sera from 31 patients with rheumatoid arthritis (RA) and from 49 patients with Yersinia enterocolitica 0:3 infection were examined for the presence of rheumatoid factors (RF) and antibodies against denatured (ss) DNA and Y. enterocolitica 0:3 lipopolysaccharide (LPS), by using enzyme-linked immunosorbent assays. Anti-ssDNA antibodies of IgG class were found equally often in the RA as in yersinia patients. The IgM-anti-ssDNA antibody levels were also elevated in the yersinia patients, whereas the RA patients had elevated levels of IgA-anti-ssDNA antibodies. High levels of RF were seen in the RA patients and an increase in IgA-RF activity was observed during the acute stage in the yersinia arthritis patients. An overlap in the serological profiles of RA and yersinia arthritis was further seen in th results of the anti-Y. enterocolitica LPS determinations, a anti-LPS antibodies of IgG class were present not only in yersiniosis but also in 16% of the RA patients.
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PMID:Common serological features in rheumatoid arthritis and yersinia arthritis. Demonstration of rheumatoid factors and antibodies against ssDNA and Yersinia enterocolitica Lipopolysaccharide by ELISA. 724 83

Phenol-water-extracted lipopolysaccharide (LPS) from Yersinia enterocolitica (strain 75 in smooth form; O-group I), after growth at 10 degrees C, contains approximately 40% (w/w) 6-deoxy-L-altrose. By increasing the cultivation temperature a significant decrease in the O-specific sugar is observed. LPS from cells grown at 40 degrees C contains about 12% 6-deoxy-L-altrose. Thin-section micrographs of cells grown at lower temperatures reveal a distinct layer corresponding to the O-specific side chains of LPS in contrast to cells grown at higher temperatures where a decrease in thickness is observed. It is assumed that this observation is due to the decreasing amount of the O-specific sugar at a higher cultivation temperature. The results obtained by the indirect immunoferritin test indicate that the O-specific polysaccharide layer covers the cell surface completely up to 37 degrees C. However, this layer no longer covers the entire surface of all cells as soon as an incubation temperature of 40 degrees C is applied.
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PMID:[Sugar composition of the lipopolysaccharide and ultrastructural study of the outer membrane of Yersinia enterocolitica (author's transl)]. 742 46

The O side chain (O antigen) of the lipopolysaccharide of Yersinia enterocolitica serotype O:3 has been shown to be a virulence factor. The genes directing the biosynthesis of the O antigen have been cloned, sequenced and characterized. Like the expression of most of the virulence factors of Y. enterocolitica, O-antigen expression is temperature regulated.
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PMID:Yersinia enterocolitica lipopolysaccharide: genetics and virulence. 751 77

Production of hematopoietic growth factors by endothelial cells plays a pivotal role during inflammatory processes. Stem cell factor (SCF) is known to be expressed constitutively in endothelial cells. To investigate the regulation of this cytokine expression by inflammatory stimuli, we cocultured human umbilical vein endothelial cells (HUVEC) with various gram-negative bacterial strains (Escherichia coli, Yersinia enterocolitica, Chlamydia trachomatis, and Neisseria meningitidis, respectively). Experiments were performed with bacterial concentrations ranging from 10(2) to 10(7) bacteria/mL for 3 hours. SCF-specific mRNA expression was studied using Northern blot analysis. Stimulation with the enteropathogenic bacterial strains Y enterocolitica and E coli resulted in a significant concentration-dependent increase of SCF mRNA expression. Similar results were obtained in coculture experiments with N meningitidis. As shown in experiments with E coli, the accumulation of SCF transcripts was also time-dependent. In contrast, coculture of HUVEC with the intracellular gram-negative strain C trachomatis had no effect on SCF mRNA expression. To elucidate the role of the gram-negative bacterial cell wall components, we stimulated HUVEC with bacterial lipopolysaccharide (LPS). LPS induced a maximal SCF mRNA accumulation within 2 hours followed by decrease of SCF-specific transcripts to the basal level after 24 hours. In addition, we exposed HUVEC to the classical inflammatory cytokine interleukin-1 alpha (IL-1 alpha). Kinetic experiments showed a similar pattern of regulation with an increase of SCF mRNA within 2 hours, persisting up to 12 hours, and a decrease to basal transcription after 24 hours. From these data, we conclude that SCF expression is regulated by inflammatory stimuli, such as IL-1 alpha and bacterial pathogens, suggesting an important role of SCF during inflammation.
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PMID:Differential regulation of stem cell factor mRNA expression in human endothelial cells by bacterial pathogens: an in vitro model of inflammation. 751 47

The O antigen obtained from the lipopolysaccharide of Yersinia ruckeri serotype 01, by mild acid hydrolysis, is composed of a branched tetrasaccharide repeating unit containing 2-acetamidino-2,6-dideoxy-L-galactose (L-FucAm), 2-acetamido-2-deoxy-D-glucose (D-GlcNAc), and 7-acetamido-3,5,7,9-tetradeoxy-5-(4-hydroxybutyramido)-D-glycero-L -galacto- nonulosonic acid (L-Sug). Partial hydrolysis of the O antigen with 0.1 M HClafforded a trisaccharide and a tetrasaccharide having nonulosonic acid at their reducing ends. Cleavage of the O antigen with anhydrous methanolic hydrogen fluoride afforded the methyl glycoside derivatives of a trisaccharide and a tetrasaccharide. 1H and 13C NMR analysis, including 1H-13C heteronuclear multiple bond correlation spectroscopy to locate the N-acyl substituents, together with mass spectrometric analysis of the above oligosaccharides, allowed the structure of the O-specific polysaccharide to be assigned as: [formula: see text].
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PMID:The structure of the lipopolysaccharide O antigen from Yersinia ruckeri serotype 01. 751 97


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