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Query: UNIPROT:P43026 (lipopolysaccharide)
 62,215 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Yersinia enterocolitica serotype 9 contained an antigenic component giving a reaction of total identity with Brucella native hapten and polysaccharide B. This component was present in a phenol-water extract (fraction 5; M. Redfearn, Ph.D. Thesis, University of Wisconsin, Madison, 1960) along with the smooth lipopolysaccharide. The native hapten could be purified free of lipopolysaccharide and proteins by gel filtration.
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PMID:Immunological identity of brucella native hapten, polysaccharide B, and yersinia enterocolitica serotype 9 native hapten. 618 20

Growth temperature affected the structure of Yersinia enterocolitica Ye 3827 lipopolysaccharide (LPS). Although Y. enterocolitica Ye 3827 synthesized smooth LPS when grown at a low temperature (25 degrees C), partial smooth-rough transition occurred when the bacteria were grown at the physiological temperature (37 degrees C). The structural alteration was detected by bacteriophage-inactivation assay and chemical and immunological analyses. LPS prepared from bacteria grown at 25 degrees C inactivated a number of bacteriophages that recognize the O-antigenic polysaccharide portion of LPS, whereas more than 3000 times the amount of LPS from bacteria grown at 37 degrees C was required for the same degree of inactivation. The antigenic determinant(s) responsible for the major reaction between 25 degrees C-LPS and anti-25 degree C-bacteria was located on the O-antigenic polysaccharide portion of LPS, but those responsible for the major reaction between 37 degrees C-LPS and anti-37 degrees C-bacteria were located on the R-core or inner portion of LPS.
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PMID:Growth temperature-dependent variation in the bacteriophage-inactivating capacity and antigenicity of Yersinia enterocolitica lipopolysaccharide. 619 8

The phenol-phase soluble cellular lipopolysaccharide isolated by the phenol/water extraction method from Yersinia enterocolitica serotype O:9 cells was shown by hydrolytic, periodate oxidation, methylation and nuclear magnetic resonance studies to be an S-type lipopolysaccharide with a linear O-antigenic polysaccharide of 1,2-linked 4,6-dideoxy-4-formamido-alpha-D-mannopyranosyl units. The serological cross-reactivity between Y. enterocolitica serotype O:9 and the lipopolysaccharides of Vibrio cholerae and Brucella species can now be related to the presence of N-acylated 4-amino-4,6-dideoxy-alpha-D-mannopyranosyl residues in their respective O-antigenic chains.
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PMID:Structure of the O-chain of the phenol-phase soluble cellular lipopolysaccharide of Yersinia enterocolitica serotype O:9. 619 99

An O-specific polysaccharide from the lipopolysaccharide Yersinia pseudotuberculosis 1A serovar has been isolated and characterized. This compound was shown to contain residues of paratose, 6-deoxy-D-manno-heptose, D-galactose and 2-amino-2-deoxy-D-glucose in equimolar ratios. Using methylation studies, partial acid hydrolysis and 13C NMR spectroscopy, the following structure was proposed for the repeating unit of the O-specific polysaccharide: (Formula: see text).
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PMID:[Structure of O-specific polysaccharide isolated from the Yersinia pseudotuberculosis serotype 1A lipopolysaccharide]. 620 36

The comparative studied on lipopolysaccharides from Yersinia pseudotuberculosis IVA serovar, strains 32 and 31D, have been conducted. The identity of the lipopolysaccharides isolated from these strains has been shown. The structural pattern of the repeating unit of the O-specific side chain of the lipopolysaccharide has been suggested: (Formula: see text)
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PMID:[Study of a lipopolysaccharide from Yersinia pseudotuberculosis of the IVA serotype]. 620 39

A 13C NMR spectrum of O-specific polysaccharide isolated from Yersinia pseudotuberculosis III serovar lipopolysaccharide has been interpreted. This allowed to define more precisely the configuration of glycosidic bonds and to confirm the structure of the repeating unit of the specific polysaccharide which was earlier established by other methods.
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PMID:[13C-NMR spectrum of O-specific polysaccharide from the lipopolysaccharide of Yersinia pseudotuberculosis of serotype III]. 620 40

Cells of all Brucella species were agglutinated by the basic protein-reactive lectins of Mangifera indica and Persea americana. Cells of non-smooth strains including B. ovis and B. canis but not smooth strains were also agglutinated by concanavalin A. These lectins also reacted in a similar pattern with lipopolysaccharide extracts of these organisms. The lectin from Bandeiraea simplicifolia precipitated with the lipopolysaccharide antigens of smooth Brucella organisms and with those of serologically cross-reactive strains of Escherichia coli, Pseudomonas maltophilia, Salmonella and Yersinia enterocolitica but not with antigenically unrelated strains.
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PMID:The interaction of Brucella cell surface components with plant agglutinins. 620 71

Using methylation studies, partial hydrolysis and 13C NMR spectroscopy data, the following structure of O-specific polysaccharide from lipopolysaccharide of Yersinia pseudotuberculosis VI serovar has been proposed: (Formula: see text).
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PMID:[Structure of O-specific polysaccharide from Yersinia pseudotuberculosis of serotype VI lipopolysaccharide]. 621 95

The expression of several virulence determinants of Yersinia pestis is known to be dependent on the in vitro growth temperature. One of these, calcium dependence, is associated with the presence of a 47-megadalton plasmid. We have examined the effects of incubation temperature, calcium in the growth medium, the presence of the 47-megadalton plasmid on the outer membrane protein, and the lipopolysaccharide composition of Y. pestis EV76. When cells were grown at 37 degrees C as opposed to 26 degrees C, a change in lipopolysaccharide composition and a decrease in the amount of an outer membrane protein (protein E) were observed. The lipopolysaccharide obtained from cells incubated at 37 degrees C had a lower proportion of 2 keto-3-deoxyoctanate, a lower phosphate to 2-keto-3-deoxyoctanate ratio, and an increased gel mobility upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis when compared with lipopolysaccharide obtained from cells grown at 26 degrees C. Because of its growth temperature-related abundance, we investigated the nature of protein E. This protein had physical properties similar to those of other enterobacterial porins, including apparent formation of an oligomer on sodium dodecyl sulfate-polyacrylamide gels when solubilized at low temperature, acidic isoelectric point, and strong noncovalent association with the peptidoglycan. Protein E was purified and shown to form an aqueous channel in planar lipid membranes with a conductance of 1.1 nS in 1 M KCl. In addition to growth temperature-related alterations in the lipopolysaccharide and porin components of the outer membrane, the amount of three spots in two-dimensional polyacrylamide gels was shown to be related to the temperature or the presence of calcium during growth. One of these spots was shown to contain residual unmodified portions of two major heat-modifiable proteins which failed to shift to their heat-modified positions on gels, despite solubilization at 100 degrees C for 10 min before electrophoresis. The other two spots were the heat-modified and unmodified forms of another outer membrane protein (J) which did not appear in the isoelectric focusing gel of cells grown at 37 degrees C. It is proposed that the appearance of these spots in two-dimensional analyses is related to the lipopolysaccharide composition of the cells from which the outer membrane is derived and reflects lipopolysaccharide-protein interactions or calcium-protein interactions.
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PMID:Effects of growth temperature, 47-megadalton plasmid, and calcium deficiency on the outer membrane protein porin and lipopolysaccharide composition of Yersinia pestis EV76. 631 90

An enzyme-linked immunosorbent assay (ELISA) was developed for determination of the IgA, IgM and IgG antibody responses against a lipopolysaccharide antigen representing Shigella sonnei phase I bacteria. Two or more sera from 33 patients infected with Shigella sonnei were collected during a 12 month period after onset of the disease. Convalescent sera from 56 patients with other enteric infections (salmonellosis, yersiniosis, campylobacteriosis) and sera drawn from 40 healthy blood donors served as controls. Twenty-eight of the 33 patients (85%) had at least one serum specimen where two or three of the immunoglobulin titres were classified as positive (greater than + 2SD above mean titres seen in healthy blood donors), whereas only ten of 56 patients (18%) with other enteric infections had similarly elevated titres (p less than 0.001). The Shigella sonnei ELISA using purified lipopolysaccharide as antigen is considered more sensitive and specific than the formerly used agglutination tests.
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PMID:Antibody response to Shigella sonnei infection determined by an enzyme-linked immunosorbent assay. 634 88


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