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Query: UNIPROT:P43026 (lipopolysaccharide)
 62,215 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In DOC-PAGE, lipopolysaccharide (LPS) of Proteus mirabilis R14/1959 (Rb-type) mutant showed a ladder-like migration pattern indicating the presence of a high molecular weight polysaccharide chain. The isolated polysaccharide, called T-antigen because of similarity with the T1 chain of Salmonella friedenau LPS, contained D-glucose, D-galacturonic acid (D-GalA), and D-GlcNAc in molar ratios 2:1:1 and was structurally different from the O-antigen of the parental S-strain P. mirabilis S1959 but identical to the O-antigen of another S-strain Proteus penneri 42. The importance of a D-GalA(L-Lys)-containing epitope, most likely present in the core region of LPS, and of GalA present in the T-antigen chain in manifesting the serological specificity of P. mirabilis R14/1959 were revealed using rabbit polyclonal homologous and heterologous R- and O-specific antisera and the appropriate antigens, including synthetic antigens which represent partial structures of various Proteus LPS.
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PMID:Structural and immunochemical studies on the lipopolysaccharide of the 'T-antigen'-containing mutant Proteus mirabilis R14/1959. 873 Oct 19

Based on acid hydrolysis, methylation, and one- and two-dimensional 1H- and 13C-NMR spectroscopy, including homonuclear and 1H, 13C heteronuclear correlation spectroscopy (COSY) and rotating-frame nuclear Overhauser effect spectroscopy (ROESY); it was found that the O-specific polysaccharide chain of the lipopolysaccharide of the bacterium Proteus mirabilis O30 is a hexosamino-glucuronan built up of tetrasaccharide repeating units having the following structure: [formula: see text] The degree of O-acetylation of 2-acetamido-2-deoxyglucose is about 70%.
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PMID:[Structure of the O-specific polysaccharide from Proteus mirabilis O30]. 875 67

A panel of monoclonal antibodies (MAbs) directed against lipopolysaccharide (LPS) of Salmonella serogroups A, B, and D was generated. Nine most productive hybrid clones were selected from several fusions of mouse myeloma cells with splenocytes from BALB/c mice, immunized with the corresponding heat-killed bacteria. The MAbs were characterized by enzyme immunoassay, Western blot analysis, and dot-immunoblotting with LPS and whole bacteria of Salmonella serogroups A-E and some other representatives of the Enterobacteriaceae family. Seven MAbs were reactive with the sole Salmonella strain used as an immunogen; one MAb, SD:10D9H, reacted with the five major serogroups of Salmonella species (A, B, D, E1, and E2); and one MAb, SA:5D12A, reacted with Salmonella serogroups A-E and a rough strain of S. cholerae-suis. None of the MAbs reacted with LPS of E. coli 055:B5 or whole bacteria of E. coli K12, Klebsiella pneumoniae, or Proteus vulgaris. The typical ladder-like patterns of bands were observed after immunoblotting of MAbs against electrophoretically resolved LPS from Salmonella serogroups A-E, which thus confirmed their LPS-directed specificity. MAbs affinity constants were determined by noncompetitive enzyme immunoassay using serial dilutions of both LPS as antigen (coating the plate) and antibodies. On the base of the results obtained, the presumed epitopes for each of the MAbs were discussed. The usefulness of MAbs generated for diagnostic and protective purposes was declared.
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PMID:Monoclonal antibodies directed against unique and common determinants on the lipopolysaccharide molecule of Salmonella serogroups A, B, and D. 877 Jun 43

The chemical structure of the O-specific polysaccharide chain of Proteus penneri 62 lipopolysaccharide (LPS) containing N-acetylisomuramic acid was established using acid hydrolysis, solvolysis with anhydrous hydrogen fluoride and 1H and 13C NMR spectroscopy. Cross reactivity of the anti-O-serum P. penneri 62 with a number of other strains of the same species isolated in the USA, Canada, Germany and Poland is discussed.
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PMID:Structure of the O-specific polysaccharide chain and serological characterization of the Proteus penneri 62 lipopolysaccharide compared with the lipopolysaccharides of the P. penneri strains. 891 24

An acidic O-specific polysaccharide from the lipopolysaccharide of Proteus mirabilis O10 contains 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, D-galacturonic acid, and L-altruronic acid, the last-named sugar having not been found hitherto in O-antigens. Structure of a branched tetrasaccharide repeating unit of the polysaccharide was established by 1H and 13C NMR spectroscopy, including two-dimensional COSY and rotating-frame NOE spectroscopy. The lateral L-altruronic acid residue plays the immunodominant role in manifestation of the O10 specificity of Proteus, whereas a disaccharide fragment of the main chain in common with the O-specific polysaccharide of P. mirabilis O43 provides the one-way serological cross-reactivity between anti-O10 serum and O43-antigen.
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PMID:Structural and serological studies of the O-specific polysaccharide of the bacterium Proteus mirabilis O10 containing L-altruronic acid, a new component of O-antigens. 897 26

The influence of type of bacterial culture media on antibiotic resistance of Proteus mirabilis R and S forms, was tested. P. mirabilis S1959 (S form), R45 and R110 strains (Re and Ra mutant, respectively) cultivated in media supplemented with 10% heat inactivated bovine serum were resistant to ampicillin, amoxicillin, nalidixic acid and nitroxoline. Proteus strains cultivated in media without serum were sensitive to these antibacterial agents. The presence of serum did not change the polymyxin E (colistin) resistance of there Proteus strains tested. The effects of the presence of colistin (1000 U/ml) in culture media on Proteus lipopolysaccharide composition was studied. The content of uronic acids and phosphate residues in lipopolysaccharides isolated from bacteria cultivated in the presence of colistin (LPS-col), were lower than in control LPSs. The contents of 4-amino-4-deoxy-L-arabinose decreases in S1959 LPS-col, increases in R110 LPS-col and remains unchanged in R45 LPS-col. These results indicate that the presence of colistin in cultivation media exerts an influence on the contents of charged components of LPSs isolated from polymyxin E-resistant Proteus R and S strains.
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PMID:Studies of antibiotic resistance of rough and smooth Proteus mirabilis strains and influence of polymyxin E on their lipopolysaccharide composition. 899 93

An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Proteus mirabilis O10 and studied after full acid hydrolysis and carboxyl reduction by 1H- and 13C-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected heteronuclear 1H,13C multi-quantum coherence (HMQC), and rotating-frame nuclear Overhauser effect spectroscopy (ROESY). It was found that the polysaccharide contains 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, D-galacturonic acid, and L-altruronic acid, and the following structure of the branched tetrasaccharide repeating unit of the polysaccharide was established: [sequence: see text]
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PMID:[Structure of O-specific polysaccharide from Proteus mirabilis O10, containing a new component of O-antigens--L-altruronic acid]. 899 79

In this work an epitope has been determined on lipopolysaccharide of Proteus mirabilis O43. This epitope is recognized by specific antibodies.
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PMID:[Serologic cross reactions between strains of Proteus mirabilis belonging to serogroup O43 I O10]. 907 67

The object of this review is the genus Proteus, which contains bacteria considered now to belong to the opportunistic pathogens. Widely distributed in nature (in soil, water, and sewage), Proteus species play a significant ecological role. When present in the niches of higher macroorganisms, these species are able to evoke pathological events in different regions of the human body. The invaders (Proteus mirabilis, P. vulgaris, and P. penneri) have numerous factors including fimbriae, flagella, outer membrane proteins, lipopolysaccharide, capsule antigen, urease, immunoglobulin A proteases, hemolysins, amino acid deaminases, and, finally, the most characteristic attribute of Proteus, swarming growth, enabling them to colonize and survive in higher organisms. All these features and factors are described and commented on in detail. The questions important for future investigation of these facultatively pathogenic microorganisms are also discussed.
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PMID:Potential virulence factors of Proteus bacilli. 910 65

Based on monosaccharide analysis and 1H- and 13C-NMR spectroscopy, the following structure of the O-specific polysaccharide chain of Proteus vulgaris OX2 lipopolysaccharide (LPS), which defines the O2 specificity of Proteus, was established: [formula: see text] where L-QuiNAc is N-acetyl-L-quinovosamine (2-acetamido-2,6-dideoxy-L-glucose). Various strains of P. vulgaris OX2 used in the Weil-Felix test for serodiagnosis of rickettsiosis (spotted fevers, except for Rocky Mountain spotted fever) were shown to produce LPS with the same O-specific polysaccharide, which differs structurally and serologically from LPS of P. vulgaris OX19 used as antigen for serodiagnosis of typhus and Rocky Mountain spotted fever. O-Acetyl groups present in the polysaccharide are not important for manifesting the immunospecificity. ELISA confirmed that the epitope responsible for the cross-reactivity between sera from patients with Japanese spotted fever and P. vulgaris OX2 cells is located on the P. vulgaris LPS. At the same time, no cross-reaction was observed between rabbit anti-P. vulgaris OX2 antibodies and the spotted fever group (SFG) rickettsial cells. Therefore, human anti-SFG rickettsial antibodies and rabbit anti-P. vulgaris OX2 antibodies may bind to distinct epitopes on P. vulgaris OX2 LPS, and no epitope recognized by rabbit anti-P. vulgaris OX2 antibodies is present on the LPS or any other surface antigen of SFG rickettsiae.
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PMID:Structural and serological studies of the O-antigen of the bacterium Proteus vulgaris OX2 (serogroup O2) used in the Weil-Felix test. 911 24


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