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Query: UNIPROT:P43026 (lipopolysaccharide)
 62,215 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

This study examines the bactericidal activity of normal human sera from individuals of blood groups A and B for gonococcal strains with simple and complex lipopolysaccharides (defined by pyocin-sensitivity) isolated from localised and disseminated infection. The bactericidal activity did not depend on A or B isohaemagglutinins. Resistance to normal human serum exhibited by strains from localised infections appeared to be due to lack of part of the lipopolysaccharide antigen, whereas resistance of strains from disseminated infection appeared to depend on a separate mechanism yet to be defined.
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PMID:Blood groups and susceptibility to gonococcal infection. II. The relationship of lipopolysaccharide type to gonococcal sensitivity to the bactericidal activity of normal human serum. 641 72

The elicitation of antibodies to gonococcal lipopolysaccharide (LPS) was studied. Rabbits were immunized with whole cells of gonococci, purified LPS, or LPS linked to bovine serum albumin with glutaraldehyde. Purified LPS was not immunogenic. Anti-LPS antibodies were produced by rabbits receiving the LPS-BSA conjugate. These animals showed an earlier IgG anti-LPS antibody response than animals receiving whole bacterial cells. Antiserum to the LPS-BSA conjugate gave rise to a single precipitation line against ultrasonically disrupted gonococci, and agglutinated heat-treated cells of the bacteria.
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PMID:Antibody response in rabbits to gonococcal lipopolysaccharide-bovine serum albumin conjugates. 641 44

The chemical and immunochemical properties of lipopolysaccharides (LPS) isolated from pyocin 103-sensitive and -resistant Neisseria gonorrheae were investigated. Marked differences were found in immunochemical behavior of LPS from pyocin-sensitive gonococcal strain JW31 and its isogenic pyocin-resistant variant JW31R. JW31 LPS readily precipitated wheat-germ agglutinin, soybean lectin, and rabbit anti-Streptococcus faecalis or horse anti-type 14 pneumococcal antibody. In contrast, JW31R LPS precipitated only soybean lectin. The combining-site specificity of anti-S. faecalis cross-precipitated by JW31 LPS, or type 14 pneumococcal capsular polysaccharide, was examined by hapten inhibition, and lactose found to be the most potent inhibitor. Horse anti-pneumococcal type 14 antibodies, cross-precipitated by JW31 LPS and streptococcal lactose polymer, exhibited heterogeneity with respect to combining site specificity. Gel filtration of LPS-derived core oligosaccharide showed both strain JW31 and JW31 R to possess R-type lipopolysaccharide with cores having a Mr approximately 1800. JW31R LPS contains more galactose but less hexosamine than JW31 LPS. Both JW31 and JW31R core oligosaccharides possess D-glucosamine and D-galactosamine, probably N-acetylated, as the only nonreducing end-groups, and (1 leads to 4)-linked D-glucose residues. Chemical data support immunochemical findings which indicate that lactose units occur as a structural feature of JW31 gonococcal LPS.
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PMID:Chemical and immunochemical studies on lipopolysaccharides from pyocin 103-sensitive and -resistant Neisseria gonorrhoeae. 641 2

Intraarticular injections of viable N, gonorrhoeae, killed N. gonorrhoeae or gonococcal lipopolysaccharide (LPS) in rabbits' knees caused an acute, polymorphonuclear synovitis with abscess formation 24-72 h after the injection. At 5-7 days, a mononuclear infiltration with synovial lining cell hyperplasia developed, which in some rabbits persisted for one month. Gonococcal LPS, in amounts of 5 micrograms or greater, always caused a marked synovitis indistinguishable from that produced by viable N. gonorrhoeae. Gonococcal outer membrane protein used as a control in these experiments caused no or minimal synovitis in concentrations 50-fold higher than those used in the LPS inoculation experiments. These studies should provide a model to investigate the role of LPS in the arthritis associated with gonococcal infection.
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PMID:Arthritis in rabbits induced by killed Neisseria gonorrhoeae and gonococcal lipopolysaccharide. 642 41

Purified disaccharide peptide monomers obtained from Neisseria gonorrhoeae by enzymatic digestion of gonococcal peptidoglycan damaged the mucosa of human fallopian tubes in organ culture. Two peptidoglycan fragments were tested: a nonreducing, anhydromuramyl-containing monomer (the principal fragment shed by growing gonococci) and the analogous reducing, muramidase-derived monomer. The damage produced by either of these peptidoglycan monomers resulted in sloughing of ciliated cells from the mucosa and resembled the damage observed in active gonococcal infection and that produced by filter-sterilized toxic supernatant fluids from gonococcal-infected organ cultures. The minimal toxic dose of peptidoglycan monomers was 0.75 micrograms/ml. Neither lipopolysaccharide, sodium dodecyl sulfate, nor Triton X-100, possible contaminants from the monomer-purification procedures, was present in sufficient quantity to account for the damage. Both of the gonococcal peptidoglycan monomers may be present in vivo and thus may play a role in the pathogenesis of gonococcal infection.
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PMID:Ability of monomeric peptidoglycan fragments from Neisseria gonorrhoeae to damage human fallopian-tube mucosa. 642 21

The ability of gonococcal R-type lipopolysaccharide (LPS) to function as an adjuvant and as an antigen in the formation of IgE and IgG1 antibody responses in mice was investigated. LPS failed to induce LPS-specific IgE or IgG1 under a variety of experimental conditions. LPS was capable of enhancing IgE and IgG1 antibody responses to gonococcal protein (ZAB), but its adjuvant effect was weaker than that of A1(OH)3. The LPS-induced anti-ZAB IgE antibody titers showed a cycling phenomenon with time, but the IgG1 response was delayed and peaked only once.
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PMID:Adjuvant and immunogenic properties of gonococcal R-type lipopolysaccharide in reaginic antibody formation in mice. 642 18

Immunisation of ddY mice with the purified ribosomal fraction of Neisseria gonorrhoeae was found to protect against intravaginal challenge with homologous organisms. This protection correlated with the presence of bactericidal antibody to purified ribosomal fraction in serum as well as in vaginal secretions. Analysis of the vaginal fluids from control mice and those immunised with purified ribosomal fraction showed that the enhanced elimination of gonococci in immune mice might be because of an early response of leucocytes generated by the reaction mediated by antibody and complement. Absorption studies showed that there was at least one major protective antigen in purified ribosomal fraction, other than cell surface substances such as lipopolysaccharide, outer membrane proteins, and pili. Bactericidal assays mediated by antibody and complement showed that matched samples of serum and vaginal fluid from immune mice had comparable gonococcidal activity, which was augmented by the effect of progesterone. Although delayed hypersensitivity was produced in immune mice that were resistant to N gonorrhoeae, the exact role of cellular immunity could not be clarified in this study. These results suggest that antibody to purified ribosomal fraction plays a major part in protection against gonococcal infection in the genital tract, and that such protection may entail both cellular immunity and hormonal changes.
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PMID:Analysis of immune responses in genital tracts of mice immunised with purified ribosomal fractions of Neisseria gonorrhoeae. 643 Apr 62

This study indicates that the gonococcal strains of lipopolysaccharide group (II) most frequently isolated from localized infections have a growth advantage over strains of group I. Their increased association with neutrophil polymorphs and their susceptibility to lysis by normal human serum would seem to act to the disadvantage of the bacteria. Our findings taken together with the cytotoxic effect of gonococci on neutrophil polymorphs offer an explanation for this apparent paradox.
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PMID:Host-parasite interactions influencing establishment of gonococcal infection--a paradox resolved? 643 93

Gonococci damaged the mucosa of human fallopian tubes in organ culture (FTOC), producing characteristic pathologic features. Filter-sterilized supernatant fluid from donor gonococcal-infected FTOC damaged recipient FTOC in a similar fashion. Gonococcal lipopolysaccharide (LPS) was detected in these toxic donor fluids in concentrations of 1.2 to 8.3 microgram/ml. Purified gonococcal LPS in concentrations as low as 0.015 microgram/ml produced damage equivalent to that caused by toxic donor fluid and was neutralized by polymyxin B. Such LPS-mediated damage to ciliated cells, if it occurs in gonococcal salpingitis, may impair mucociliary flow and predispose to ectopic pregnancy and recurrent ascending infection.
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PMID:Gonococcal lipopolysaccharide: a toxin for human fallopian tube mucosa. 678 51

Infection of human fallopian tubes in organ culture with Neisseria gonorrhoeae causes extensive damage of the mucosa. Filter-sterilized supernatant from gonococci-infected organ cultures produced similar damage in recipient uninfected organ cultures. This observation indicated the presence of one or more toxic factors. The toxic activity was unchanged after heating the supernatant to 85 C and was only partially diminished by dialysis. Toxic activity could not be detected in homogenates of uninfected organ cultures but was present in supernatants of gonococcal broth cultures. Toxic supernatants from organ cultures contained microgram quantities of gonococcal lipopolysaccharide (LPS). Amounts of this LPS and toxic activity for genital mucosa were both substantially reduced by absorption of the supernatant with limulus amoebocyte lysate. Thus, gonococcal LPS appears to be responsible for most of the toxicity of filter-sterilized supernatant from gonococci-infected human fallopian tubes in organ culture and may play an important role in the pathogenesis of gonococcal infection in vivo.
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PMID:Studies of toxicity of Neisseria gonorrhoeae for human fallopian tube mucosa. 678 64


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