Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P42574 (
caspase-3
)
45,978
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
This study was purposed to explore the apoptotic effect of gambogic acid on Raji cells and the role of
death inducer-obliterator 1
(
DIO-1
) in this process. Annexin V-fluorescein-isothiocyanate/propidium iodide was used to detect apoptosis of Raji cells. Western blot was used to determine the expressions of
DIO-1
, Bcl-xL, pro-
caspase 3
and 2 activated subunits: P17 and P20. The subcellular localization of
DIO-1
in untreated and treated Raji cells was checked by immunofluorescence and Hoechst 33258 double staining. The results showed that the Gambogic acid dose-dependently induced the apoptosis of Raji cells, downregulated the expression of Bcl-xL, upregulated the expressions of
DIO-1
and pro-
caspase 3
, induced the cleavage of pro-
caspase 3
and DIO nuclear translocation. It is concluded that gambogic acid induces the apoptosis of Raji cells through
DIO-1
upregulation, nuclear translocation, Bcl-xL downregulation and
caspase 3
activation.
...
PMID:[Mechanism of gambogic acid-induced apoptosis in Raji cells]. 1923 54
