Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P42574 (
caspase-3
)
45,978
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
BACKGROUND
E74-like factor 5
(
ELF5
) plays a key role in the processes of cell differentiation, apoptosis, and occurrence of tumors. However, the effect of
ELF5
on metastasis and invasion in human ovarian cancer remains poorly understood. MATERIAL AND METHODS Quantitative real-time polymerase chain reaction (qPCR) was performed to measure the expression of
ELF5
. The viability of cells was detected by cell counting kit (CCK-8). Cell apoptosis was tested by flow cytometry. Matrigel plug angiogenesis assay was employed to determine angiogenesis rate. The protein expression levels of vascular endothelial growth factor (VEGF), cleaved
caspase-3
, B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), E-cadherin, N-cadherin, Snail, phosphoinositide 3 kinase (PI3K), phosphorylated (p)-PI3K, tyrosine kinase B (AKT), and phosphorylated (p)-AKT were determined by Western blot. Wound-healing assay and Transwell were used to determine invasion and migration. RESULTS We found that expression of
ELF5
was obviously decreased in ovarian cancer cell lines. The cells viability, invasion and metastasis were inhibited by overexpression
ELF5
.
ELF5
suppressed angiogenesis rate and the expression of VEGF. Changes of the expressions of Bcl-2, cleaved
caspase-3
and Bax showed that anti-apoptosis ability was improved by
ELF5
.
ELF5
also repressed N-cadherin and Snail and increased E-cadherin. The expressions of p-PI3K and p-AKT were decreased by
ELF5
. Further study showed that IGF-I reversed the inhibitory effect of
ELF5
on growth and metastasis of SKOV3 cells. CONCLUSIONS Overexpression of
ELF5
promoted the apoptosis and reduced the migration and invasion of ovarian cancer cells; therefore, it could provide a new approach to gene treatment of ovarian carcinoma.
...
PMID:Overexpression of E74-Like Factor 5 (ELF5) Inhibits Migration and Invasion of Ovarian Cancer Cells. 3069 3
