Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P42345 (
mTOR
)
26,049
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Cerium oxide nanoparticles (CeO
2
NPs) possess powerful antioxidant properties, thus emerging as a potential therapeutic tool in non-alcoholic fatty liver disease (NAFLD) progression, which is characterized by a high presence of reactive oxygen species (ROS). The aim of this study was to elucidate whether CeO
2
NPs can prevent or attenuate oxidant injury in the hepatic human cell line HepG2 and to investigate the mechanisms involved in this phenomenon. The effect of CeO
2
NPs on cell viability and ROS scavenging was determined, the differential expression of pro-inflammatory and oxidative stress-related genes was analyzed, and a proteomic analysis was performed to assess the impact of CeO
2
NPs on cell phosphorylation in human hepatic cells under oxidative stress conditions. CeO
2
NPs did not modify HepG2 cell viability in basal conditions but reduced H
2
O
2
- and lipopolysaccharide (LPS)-induced cell death and prevented H
2
O
2
-induced overexpression of MPO, PTGS1 and iNOS. Phosphoproteomic analysis showed that CeO
2
NPs reverted the H
2
O
2
-mediated increase in the phosphorylation of peptides related to cellular proliferation, stress response, and gene transcription regulation, and interfered with H
2
O
2
effects on
mTOR
, MAPK/ERK,
CK2A1
and PKACA signaling pathways. In conclusion, CeO
2
NPs protect HepG2 cells from cell-induced oxidative damage, reducing ROS generation and inflammatory gene expression as well as regulation of kinase-driven cell survival pathways.
...
PMID:Cerium Oxide Nanoparticles Protect against Oxidant Injury and Interfere with Oxidative Mediated Kinase Signaling in Human-Derived Hepatocytes. 3178 79
