Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P42345 (mTOR)
 26,049 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The present study investigated the relationship between the expression of p-mTOR, p-4EBP1 and p-p70S6K in the cytoplasm and nucleus of ameloblastoma (AB) cells and the invasiveness of ABs. Immunohistochemistry was performed to detect the expression of p-mTOR, p-4EBP1 and p-p70S6K in ABs and the level of these proteins in the nucleus and cytoplasm was scored. There was ectopic expression of p-mTOR in ABs. 27 AB patients were positive for p-mTOR expression in the nucleus and 47 for p-mTOR expression in the cytoplasm. The ectopic expression of p-4EBP-1 was also noted. 23 patients (27%) were positive for p-4EBP-1 expression in the nucleus and 55 (64.7%) for p-4EBP-1 expression in the cytoplasm. The ectopic expression of p-p70S6K was also noted. Of these patients, 33 were positive for p-p70S6K expression in the nucleus (38.8%) and 45 for p-p70S6K expression in the cytoplasm (52.9%). Statistical analysis showed the expression of three proteins in the nucleus of patients with recurrent cancer was markedly higher than that in those with primary cancer. The expression of p-mTOR, p-4E-BP1 and p-p70S6K in the nucleus was related to the invasiveness of ABs. Multivariable analysis with Cox proportional hazards model showed the p-mTOR expression had influence on AB recurrence (OR: 6.417, 95%CI: 1.428-28.824). The possibility of AB recurrence in patients with nuclear p-mTOR expression was 6.417 folds higher than that in those with cytoplasmic p-mTOR expression. The nuclear expression of p-mTOR, p-4E-BP1 and p-p70S6K was associated with biological behaviors (invasiveness) of ABs, and p-mTOR was an independent predictor of AB.
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PMID:Expression of phosphorylated mTOR and its regulatory protein is related to biological behaviors of ameloblastoma. 2297 62

This study aimed to evaluate the expression of AKT and phosphorylated AKT (p-Akt) in human ameloblastoma (AB). Immunohistochemistry showed human AB was positive for Akt and Akt expression was mainly found in the cytoplasm of epithelial cells. The Akt expression in AB was significantly higher than that in normal oral mucosa (NOM), but still lower than that in oral squamous cell carcinoma (OSCC). NOM was negative for p-Akt, but AB was positive for p-Akt. In some AB tissues, p-Akt expression was found in both cytoplasm and nucleus. Akt expression in AB was significantly different from that in NOM and OSCC. The p-Akt in AB was markedly higher than that in NOM, but lower than that in OSCC. mTOR expressed in cytoplasm in AB, but not in NOM. P-mTOR expressed on cell membrane in NOM, while in cytoplasm and nucleus in Ab. Results of western blot assay showed that Akt expression was found in all the AB tissues, and increased in tissues with malignant transformation. In addition, the p-Akt expression also markedly increased in AB, but was still lower than that in OSCC tissues. Compared to NOM, mTOR and p-mTOR expression significantly increased in AB. BandScan 5.0 software was used to detect the optical density of protein bands. Results showed p-Akt, mTOR and p-mTOR expression in AB was markedly different from that in control group.
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PMID:Expression of phosphorylated Akt/mTOR and clinical significance in human ameloblastoma. 2613 Oct 97